Evaluation of genetic relationships of some soybean genotypes using morphological and molecular markers (original) (raw)

Abstract

Genetic diversity of cultivated soybean is very narrow. This may threaten the ability of breeders to sustain improvement and increase vulnerability of the crop to yields. The objective of this research was to assess genetic diversity of six genotypes i.e., Giza 21, Giza 22, Giza 111, Crawford, Toano and line H30 by morphological and molecular markers using Inter Simple Sequence Repeats (ISSR) markers, to use this information in soybean future breeding programs. Eleven ISSR primers were tested and six of them amplified clear and reproducible bands. A total of ISSR fragments were detected, of which (65.7%) were polymorphic. Based on morphological and molecular analysis the four cultivars Giza 21, Giza 22, Giza 111 and Crawford have low genetic diversity and clustered in one group. Only Toano cultivar was positioned in another group. Moreover, the line H 30 as a promising genotype was highly diverged from other genotypes. It seems to be using Toano and line H30 as distinct and exotic g...

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