Characterizing Three Component Heteromeric Complexes Mediated by Otoferlin, a Multivalent Membrane Fusion Protein, Using a Novel Single-Molecule Assay (original) (raw)

Characterizing three component heteromeric complexes mediated by otoferlin, a multivalent membrane fusion protein, is critical for understanding its role in cellular signaling. This study presents a novel single-molecule assay designed to explore how otoferlin interacts with different protein clients and how these interactions contribute to the overall function of the protein. Results indicate that otoferlin acts as a scaffold, facilitating complex assembly and signal transduction, thereby shedding light on the intricacies of multivalent protein functionality.