The chemical chaperon 4-phenylbutric acid improves cardiac function following isoproterenol-induced myocardial infarction in the rat (original) (raw)

Purpose 4-phenyl butyric acid (4-PBA) is a chaperone-mediated autophagy (CMA) inducer, which eliminates unnecessary and damaged cellular components through lysosomal enzymes. It could reduce misfolded and unfolded proteins produced after myocardial infarction (MI) and can improve cardiac function. We aimed to investigate the effect of 4-PBA on isoproterenol-induced MI in the rat. Methods Isoproterenol (100 mg/kg) was injected subcutaneously for two consecutive days simultaneous with an intraperitoneal (IP) injection of 4-PBA at 20, 40, or 80 mg/kg with 24-h intervals for five days. On day 6, hemodynamic parameters, histopathological changes, peripheral neutrophil count, and total antioxidant capacity (TAC) evaluated. The expression of autophagy proteins measured by using western blotting. 4-PBA significantly improved post-MI changes in hemodynamic parameters.Results Histological improvement found in 4-PBA 40 mg/kg (P<0.05). The neutrophils count in the peripheral blood significan...