Antimicrobial and antioxidant activities of Cortex Magnoliae Officinalis and some other medicinal plants commonly used in South-East Asia (original) (raw)
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Bangladesh Pharmaceutical Journal, 2020
The current study was designated to explore the antioxidant and antibacterial properties of the methanolic extract of Magnolia champaca stem bark and its different fractions. Antioxidant activity was assessed using total antioxidant capacity, ferric reducing power, DPPH, hydroxyl and hydrogen peroxide scavenging assay. Antibacterial activity was evaluated against five gram positive and five gram negative bacteria using disc diffusion assay method. Among the different fractions, chloroform fraction (CHF) and ethyl acetate fraction (EAF) showed the highest antioxidant activity whereas aqueous fraction (AQF) showed lowest activity in DPPH radical scavenging assay with IC 50 of 12.12, 22.41 and 55.16 µg/ml, respectively. Both of the extracts CHF and EAF also exhibited highest total antioxidant capacity, ferric reducing power and hydrogen peroxide scavenging activity with concentration dependent manner when compared to standard BHT. Moderate to potent antibacterial activity was observed against all tested organisms compared to standard azithromycin. The results from the present study revealed that the different fractions of stem bark of M. champaca specially CHF and EAF possess antioxidant and antibacterial property which support its use in traditional medicine and suggesting that the plant may be further investigated to discover its pharmacologically active natural products.
Journal of Advanced Biotechnology and Experimental Therapeutics, 2019
ABSTRACT: The aim of this study was to explore phytochemical profiling, antioxidant and antibacterial activity of four medicinal plants including Catharanthus roseus, Aegle marmelos, Moringa oleifera, and Ageratum conyzoids grown in Sylhet district, Bangladesh. In this study, total 11 phytochemicals were screened from methanol extract of four medicinal plants, wherein flavonoid, tannin, sterol, phenol were present in all four medicinal plants. In vitro, antioxidant activity of these medicinal plants extract was investigated by DPPH-radical scavenging assay. The Aegle marmelos exhibited the highest antioxidant activity followed by Moringa oleifera, Ageratum conyzoids, and Catharanthus roseus extract. Methanolic extracts of same medicinal plants were subjected to a test of their antibacterial activities against Staphylococcus aureus, Escherichia coli, Klebsiella sp., Pseudomonas sp .and Salmonella sp. by agar disc diffusion method. The highest antibacterial potential was observed in the extract of Aegle marmelos against Salmonella sp. followed by Catharanthus roseus against Pseudomonas sp .with zone of inhibition of 18.67 mm, 15.0 mm, respectively. This study confirmed the efficacy of some native medicinal plants extract as potential source of phytochemicals, along with natural antioxidant and antimicrobials, which provide new possibilities to employing them against disease causing test organisms.
International Journal of Scientific Research in Science, Engineering and Technology, 2020
Plant-derived substances have been used since antiquity for therapeutic and other purposes. The present study was undertaken to make a comparative analysis of antimicrobial and antioxidant properties of 8 medicinal plants, which are used in traditional medicine system. Methanolic extracts of Centella asiatica (leaves), Mentha piperita (leaves), Calotropis gigantean (leaves), Vitex nigundo (leaves), Bauhinia racemosus (leaves), Emblica officinalis (fruit), Bauhinia purpurea (leaves), Asperagus racemosus (stem) were tested for anti-microbial effects using the standard Agar Diffusion method. Antioxidant attributes of above extracts were studied by testing their ability to scavenge DPPH and ABTS radicles. Our results indicated that all tested extracts, with the exception of Bauhinia purpurea, were effective against E. Coli, while Candida albicans was only sensitive to Asperagus racemosus. Among tested extracts, Bauhinia racemosus was most potent in exhibiting radical scavenging activity. Our data provides rationale for exploitation of these plants for antimicrobial and antioxidant attributes.
Antimicrobial and Antioxidant Activity Analysis of Some Medicinal Plants of Bangladesh
Advances in Plants & Agriculture Research, 2015
The antimicrobial potential of eight selected medicinal plants was evaluated against medically important thirteen pathogenic bacteria and three pathogenic fungi by using disc diffusion method. Moreover, the free radical scavenging activity was investigated for the development of anti-aging ingredient as a raw product for the cosmetic and other industries. Petroleum ether, ethyl acetate, methanol, butanol, n-hexane water extracts of medicinal plants were used for antimicrobial and anti-oxidant investigation. The rapid evaluation of antioxidant activity of different crude extracts of Pouzolzia zeylanica, Equisetum debile, Memecylon umbellatum, Datura metel, Cauroupeta guianensis, Andrographis paniculata were determined by using DPPH free radical method. The microbial inhibition zone ranged from 7-15 mm for different extracts of Andrographis paniculata where ethyl acetate extract showed highest microbial inhibition. The petroleum ether extract of D. metel gave 9-10 mm microbial inhibition except Pseudomonas aureus. Aloe vera gave 7-9 mm inhibition against microorganism by ethyl acetate extract. The methanol extract of A. paniculata showed inhibition only against two gram-positive and five gram-negative bacteria. The methanol extract of P. zeylanica, M. umbellatum and butanol (n-BuOH) extract of A. vasica also showed different range of antimicrobial activity. The N-hexane extract of A. vera and A. vasica and methanol extract of Equisetum debile did not show any antimicrobial activity. These extracts resulted in a rapid increase and decrease of the absorbance and showed different hydrogen-donating capacity towards the 2, 2′-diphenyl-1-picrylhydrazyl (DPPH) radical. A lot of differences found and showing anti-oxidant activity of different solvent extracts of different plant species. Among the species, ethyl acetate extract of E. debile, M. umbellatum, methanol Extract of M. umbellatum, C. guianensis and n-BuOH extract of M. umbellatum, C. guianensis showed the maximum scavenging capacity of over 80.
Romanian Biotechnological Letters, 2021
Bioassays provide an authentication of the traditional knowledge of medicinal plants. Antibacterial, antioxidant and phenolic content of nine medicinal plants (Achillea millefolium, Prunella vulgaris, P. laciniata, Lythrum salicaria, Epilobium angustifolium, E. hirsutum, Pedicularis comosa, Agrimonia eupatoria and Verbena officinalis) were investigated in connection with folkloric usages. Aerial parts of nine plant species were extracted with water and methanol (MeOH). Disc diffusion method was performed to evaluate the antibacterial activity of the extracts against ten pathogenic bacteria. Antioxidant activity was determined by using 2,2-diphenyl-1- picrylhydrazil (DPPH) radical photometric assay. Total phenolic and flavonoid content were investigated by using Folin-Ciocaltaeu and aluminum chloride (AlCl3) colorimetric method, respectively. Quantitative analysis of phenolic constituents of nine plants species were performed by high performance liquid chromatography-diode array dete...
Arabian Journal of Chemistry, 2015
Please cite this article as: N. Akhtar, I-u. Haq, B. Mirza, Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species, Arabian Journal of Chemistry (2015), doi: http://dx.Abstract Plants are rich source of therapeutic compounds that have tremendous applications in pharmaceutical industry. To find new sources of antimicrobial and antioxidant agents, methanol/chloroform and aqueous extracts of 61 medicinal plants were evaluated systematically. Antimicrobial activity was assessed against six bacterial and five fungal strains, while natural antioxidants were studied by using reducing power (RP), total antioxidant capacity (TAC) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Six plants exhibited broad spectrum antibacterial activity while two exerted significant antifungal activity. Total phenolic content (TPC) of the samples varied from 20.2 -85.6 mg /g dry weight (DW) in M/C extracts and 5.5 -62.1 mg /g DW in aq. extracts, expressed as gallic acid equivalents (GAE). Total flavonoid content (TFC) varied from 2.9 -44.5 mg quercitin equivalent (QE) /g DW of sample 2 for M/C extracts and 2.4 -37.1 mg QE /g DW for aq. extracts. The results showed that antioxidant activities of plant species varied to a great extent not only among extracts (M/C and aq.) but also between the assays used for antioxidant evaluation. Significant linear correlation (p < 0.01) of TPC with antioxidant activities suggested their contribution to antioxidant activity. Using high performance liquid chromatography coupled with diode array detector (HPLC-DAD), gallic acid and rutin were detected in most of plant extracts with significant antioxidant activities. Study identifies plants with antimicrobial and antioxidant properties which could be used for isolation of desired therapeutic compounds and to develop infusions, nutriceuticals and pharmaceuticals. Samples of 61 medicinal plants were collected from different areas of Pakistan and identified by Professor Dr. Rizwana Aleem Qureshi, Department of Plant Sciences, Quaid-i-Azam University, Islamabad. Voucher specimens were submitted to the herbarium of Quaid-i-Azam University for future reference. The plants were selected on the basis of local use of these plants in folk medicine.
International Journal of Secondary Metabolite, 2018
This study was designed to evaluate the phenolic compounds and the in vitro antioxidant and antimicrobial activities of Onobrychis nitida, Hedysarum cappadocicum, Ebenus laguroides and Ebenus macrophylla which are medicinal plants and endemic for the flora of Turkey. The RP-HPLC-DAD (reverse phasehigh performance liquid chromatography with a diode array detector) was used to evaluate the phenolic contents. The antioxidant properties were determined to use total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH• radical scavenging activity assays. Antibacterial tests were performed against 11 different microorganisms by using the microwell dilution method. Each of the plant extracts were confirmed by bioactive assays which demonstrated a significant activity due to different chemical characteristics. Especially, rutin was the dominant component in Ebenus species with 19.434-11.808 mg phenolic/g extract. While the highest phenolic content (101.73 mg gallic acid equivalent/g extract) was observed in O. nitida, the highest FRAP value was in E. laguroides (719.09 μM FeSO4.7H2O equivalent), and the strongest DPPH degree was in the E. macrophylla extract with IC50: 69.45 μg/mL, respectively. Although plant extracts didn't have efficient values for antimicrobial activity, the slight effect was arisen in O. nitida against B. subtilis and S. aureus. The results showed that all the extracts could be used in pharmacological or dietary applications due to their valuable properties.
The anti-oxidant activity and total phenolic content of alcoholic extracts from seven medicinal plants (Asparagus racemosus, Ocimum sanctum, Cassia fistula, Piper betel, Citrus aurantifolia, Catharanthus roseus, and Polyalthia longifolia) were evaluated by using a model system consisting of β-carotene, DPPH free radical and Folin-Ciocalteu method. The total Phenolic content of the extracts was determined spectrophotometrically according to the Folin-Ciocalteu procedure and ranged from 366mg/100g to 212 mg/100g on fresh weight basis. The total flavanoid content of extracts determined by Aluminium chloride colorimetric assay and ranged from 39.84mg/100g to 15.94mg/100g of fresh weight. The highest antioxidant activity was demonstrated by Citrus auantifolia (87.05%) followed by Ocimum sanctum (81.80%) and Catharanthus roseus (71.4%). The highest tannin content was found to be in Catharanthus roseus (7.14%) while in case of anthocyanin content the highest value was found to be in Polyalthia longifolia (0.65mg/l). As far as antimicrobial activity is concerned, Ocimum sanctum and Citrus aurantifolia were found to be most potent against Escherichia coli and Staphylococcus aureus whereas Piper betel showed no effect. Except Piper betel all the extracts were able to inhibit the two bacterial strains and the zone of inhibitions ranged from 19.6 mm to 13.5 mm. The minimum inhibitory concentration against E. coli in case of Ocimum sanctum is 10% and against S. aureus it is 20% while in case of Citrus aurantifolia extracts were active even at 10% concentration for E. coli and 15% for S. aureus.
Evaluation of Phytochemical, Antioxidant and Antibacterial Activities of Selected Medicinal Plants
2021
Medicinal plants are important reservoirs of bioactive compounds that need to be explored systematically. Because of their chemical diversity, natural products provide limitless possibilities for new drug discovery. This study aimed to investigate the biochemical properties of crude extracts from fifteen Nepalese medicinal plants. The total phenolic contents (TPC), total flavonoid contents (TFC), and antioxidant activity were evaluated through a colorimetric approach while the antibacterial activities were studied through the measurement of the zone of inhibition (ZoI) by agar well diffusion method along with minimum inhibitory concentrations (MIC) by broth dilution method. The methanolic extracts of Acacia catechu and Eupoterium adenophorum showed the highest TPC (55.21 ± 11.09 mg GAE/gm) and TFC (10.23 ± 1.07 mg QE/gm) among the studied plant extracts. Acacia catechu showed effective antioxidant properties with an IC50 value of 1.3 μg/mL, followed by extracts of Myrica esculenta, ...
BMC Chemistry
Background: As possible sources of natural bioactive molecules, the plant essential oils and extracts have been used globally in new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease. Methods: In this research, the antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens, Rhaponticum repens, Alhagi maurorum, Melia azedarach, Peganum harmala, and Juncus conglomeratus were evaluated against 8 bacteria (S. aureus, B. subtilis, R. toxicus, P. aeruginosa, E. coli, P. syringae, X. campestris, P. viridiflava) and 3 fungi (Pyricularia oryzae, Fusarium oxysporum and Botrytis cinerea), through disc diffusion method. Furthermore, the essential oils of plants with the highest antibacterial activity were analyzed utilizing GC/MS. Moreover, the tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol and flavonoid of these plants was measured. Results: Among the tested plants, methanolic and chloroformic extracts of P. harmala fruits showed the highest antibacterial activity against the tested bacteria. Besides, the investigation of free radical scavenging effects of the tested plants indicated the highest DPPH, protein, guaiacol peroxidase, and catalase in P. harmala, M. azedarach, J. conglomeratus fruits, and J. conglomeratus fruits, respectively. In addition, the phytochemical analysis demonstrated the greatest amounts of total phenolic and flavonoid compositions in J. conglomeratus and P. harmala, respectively. Conclusion: The results indicated that these plants could act as a promising antimicrobial agent, due to their short killing time.