Pages 1-6 Evaluation of Antibacterial Activity of Root Canal Sealers Used in Primary Teeth Against E. faecalis (original) (raw)
Related papers
In vitro antibacterial activity of three root canal sealers against Enterococcus Faecalis
South African Dental Journal
The antibacterial activity of root canal sealers may contribute to the eradication of remaining bacteria in root canals following canal shaping and debridement. The aim of the study is to assess the antimicrobial effect of 3 endodontic sealers: Sealapex™, EndoREZ™ and Guttaflow bioseal™ against Enterococcus faecalis. This was a laboratory-based comparative study testing the antimicrobial activity of three endodontic sealers against Enterococcus faecalis. The endodontic sealers were tested unset 20 minutes after mixing and after setting. Testing after setting enabled the assessment of the antimicrobial activity of aged sealers after 7, 14, 21 and 28 days. A total of 150 samples were used for the study. The tested sealers were divided into 3 groups: • Group 1 (EndoREZ™) n = 45 plates, n = 5 control plates. • Group 2 (Guttaflow bioseal™) n = 45, n = 5 control plates. • Group 3 (Sealapex™) n = 45, n = 5 control plates. All the materials exhibited some activity against the bacteria. The ...
Antibacterial activity of different root canal sealers against Enterococcus faecalis
2017
Background: The aim of the present study was to compare in vitro the antimicrobial activity of different root canal sealers against Enterococcus faecalis, prior and subsequent to setting. Material and Methods: Agar diffusion test (ADT) was used for evaluating the antibacterial activity of non-set sealer while the direct contact test (DCT) was used for after setting. Results: ADT: Except for TotalFill BC Sealer all the others sealers tested showed antibacterial activity. BioRoot™RCS, MTA Fillapex and Sealapex Root Canal Sealer showed the lowest antibacterial activity, a significant increase in antibacterial effect for both Pulp Canal Sealer™ and AH plus sealers were found. Significantly higher were the mean diameters of the bacterial inhibition zone by both EasySeal or N2 sealers. DCT: AH plus and Sealapex Root Canal Sealer doesn't show any bactericidal effect after 6 min of contact. After 15 and 60 min of contact a significant increment for AH plus and for Sealapex Root Canal Sealer of the bactericidal effect was found. Significantly much higher was the antibacterial effect of Sealapex Root Canal Sealer compare to that observed for AH plus. BioRoot TM RCS, MTA Fillapex, Pulp Canal Sealer™ and N2 showed at least means of the number of colonies formed in milliliter after 6 min of contact. Except for N2, a significant increase in bactericidal effect after 15 and 60 min for the other compared sealers (BioRoot TM RCS, MTA Fillapex and Pulp Canal Sealer™). Conclusions: For every contact times considered, both TotalFill BC Sealer and EasySeal were bactericidal against E. faecalis and killed all bacteria.
In vitro antimicrobial efficiency of different root canal sealers against Enterecoccus faecalis
European Journal of General Dentistry, 2013
Objective: The antibacterial effectiveness of four different sealers AH Plus, EndoRez, mineral trioxide aggregate (MTA) Fillapex, iRoot SP against Enterococcus faecalis was evaluated by time kill assay method in vitro. Materials and Methods: Four sealers are used in this study: An epoxy resin-based sealer, AH Plus (Dentsply, Maillefer, Switzerland), a polymethacrylate resin-based sealer, EndoRez (Ultradent, South Jordan, UT) and two calcium silicate-based sealers, MTA Fillapex (Angelus Solucxoes Odontologicas, Londrina, Brazil), iRoot SP (Innovative BioCreamix Inc., Vancouver, Canada). Each sealer was mixed according to manufacturer′s instructions. Five mg of each sealer was added to sterile tubes separately and evaluated at 20 min, 24 h, 7 days, and 30 days. Two tubes were used as positive and negative. Results: At the 20th min AH Plus and iRoot SP were bactericidal, MTA Fillapex, and EndoRez were ineffective at the 20th min. At the 1st day MTA Fillapex was ineffective and rest of ...
Comparison of antimicrobial activity of three root canal sealers: An in vitro study
International Journal of Applied Dental Sciences, 2019
Aim and Objectives: To evaluate the antimicrobial activity of three endodontic sealers AH Plus, Apexit plus and MTA Fillapex on three types of isolated root canal microorganisms: Enterococcus Faecalis, Staphylococcus Aureus and Candida Albicans using agar diffusion test. Material and Method: Three endodontic sealers were evaluated in this study: AH Plus (Dentsply, Rio de Janeiro, RJ, Brasil), Apexit Plus (Vivadent; Schaan, Liechtenstein) and MTA Fillapex (Angelus, Londrina, PR, Brazil). The following reference strains were included in this study: Candida albicans, Enterococcus faecalis, and Staphylococcus aureus. Antimicrobial activity of the materials was evaluated by the method of agar diffusion. A base layer of 10 ml of Mueller Hinton agar was poured in Petri plates (90 x 15 mm). Inhibition halos formed around the wells were measured with the aid of a millimetric rule with 0.5 mm of accuracy. All the experiments were repeated three times in different moments. Result: In inter gro...
Comparison of antimicrobial activity of three root canal sealers
International journal of health sciences
Background Endodontic sealers with antimicrobial properties can be advantageous as they would cope better with persisting residual infection and bacteria reentering from the oral cavity. Hence we compare the antimicrobial activity of three endodontic sealers AH Plus, Apexit plus and MTA Fillapex on Enterococcus Faecalis, Staphylococcus Aureus and Candida Albicans. Material and Method : Antimicrobial activity of the materials was evaluated by the method of agar diffusion. A base layer of 10 ml of Mueller Hinton agar was poured in Petri plates (90 x 15 mm). Three wells of 6 mm of diameter (one for each material) were obtained by removing a standardized portion of the agar in equidistant points with the aid of sterilized plastic straw. The wells were immediately filled with the sealers to be evaluated. The endodontic sealers AH Plus, Apexit Plus and MTA Fillapex were prepared according to the manufacturer’s instruction. Inhibition halos formed around the wells were measured wi...
Journal of Endodontics, 2016
Introduction: The purpose of this systematic review was to summarize the outcomes of in vitro studies of root canal sealers for their antimicrobial effectiveness against Enterococcus faecalis on the basis of direct contact test. Methods: An exhaustive literature search was performed by using MEDLINE, Scopus, TRIP, and OpenThesis databases, followed by an extensive hand search of references of identified articles. Two independent reviewers evaluated the studies for eligibility against inclusion and exclusion criteria and performed data abstraction and risk of bias evaluations. Results: A total of 31 articles were assessed for eligibility. Of these, 24 met the inclusion criteria and were included in the systematic review. A considerable heterogeneity was found in the methodologies of included studies. Therefore, it was not feasible to perform metaanalysis. Majority of the studies reported that different categories of freshly prepared sealers possessed some form of positive antimicrobial activity against E. faecalis for up to 24 hours. Antimicrobial efficacy was lost as the material set. Moderate evidence was found for no antimicrobial activity of aged (2-day to 7-day set) sealer samples across all categories. Conclusions: The evidence indicated positive antimicrobial activity of freshly mixed sealers against E. faecalis. Antimicrobial efficacy was lost as the material set, with no bacterial growth inhibition by 2-day to 7-day set sealer samples. However, the studies included in this review presented medium to high risk of bias. This review identified the need for the development of standardized methods to evaluate antimicrobial activity of root canal sealers in in vitro studies.
Antimicrobial efficacy of primary root canal filling material against Enterococcus faecalis
2016
Enterococcus faecalis is associated with different root canal infections ,and it is the main cause of chronic periapical lesions. Furthermore it is difficult to eliminate it from root canal in one visit or more ,so The aim of this study was to evaluate the antimicrobial activity of root canal paste on E. faecalis. 42 extracted single rooted primary teeth were manually prepared, sterilized, and then infected with clinical isolates of E. faecalis for 30 days. bacteria were exposed to ZOE and Ledermix pastes as intracanal medication for 1 week. Finally bacterial samples were collected, and colony-forming units were enumerated. none of pastes resulted in complete elimination of bacteria. The antimicrobial effect of ZOE was significantly better than Ledermix at P=.05. this study has shown that none of the sealers totally inhibited the growth of E. faecalis , and ZOE paste had the greater reduction of the CFU count for E. faecalis cells than Ledermix
Journal of endodontics, 2006
The purpose of this in vitro study was to evaluate the antimicrobial effects of five antibiotics when added to Kerr Pulp Canal Sealer EWT against Enterococcus faecalis. Five antibiotics: amoxicillin, penicillin, clindamycin, metronidazole, and doxycycline, were added separately to Kerr sealer. Thirty brain heart infusion agar plates were inoculated with E. faecalis and sterile paper discs containing a sealer-antibiotic combination were randomly assigned to the inoculated plates. Kerr sealer on a sterile paper disc and a blank sterile paper disc served as the controls. Fifteen plates were incubated aerobically and the remaining 15 plates were incubated anaerobically at 37 degrees C. The zones of inhibition were measured at 48 hours. No significant differences (p > 0.05) were found between aerobic and anaerobic groups. The groups were combined and data analysis using a one-way ANOVA and Tukey's post hoc test was performed. Results revealed that sealer-antibiotic combinations co...
Antimicrobial activity of endodontic sealers on Enterococcus faecalis
Journal of endodontics, 2003
Enterococcus faecalis has been shown to be highly resistant once established in the root canal system and may play an important role in endodontic failures. The purpose of this study was to evaluate the antimicrobial activity of four root canal sealers on E. faecalis. Seventeen blood-agar plates were inoculated with E. faecalis using the Lawn technique. Five discs were placed on each plate, four with sealers--Sealapex, Roth 811, Kerr EWT, and AH-Plus--and an ampicillin disc as the control. The plates were incubated at 37 degrees C. The zones of inhibition were measured at 24 and 48 h. Analysis using a one-way ANOVA and Tukey test showed a statistically significance difference (p < 0.05) between all four groups of sealers. Roth 811 showed the largest zone of inhibition (1.1 mm), followed by Sealapex (0.8 mm) and Kerr EWT (0.5 mm), whereas AH-Plus had no antimicrobial activity. There was no difference in the zones of inhibition between the 24- and 48-h time periods.
Antimicrobial Activity of Bioceramic Root Canal Sealers: A Systematic Review
International journal of odontostomatology, 2021
Actually, bioceramic endodontic sealers represent an advantageous option for root canals sealing due to their multiple properties. There are several in vitro studies about their antimicrobial properties, however, their comparative antimicrobial potential, compared with other types of endodontic sealers is not entirely clear. The aim of the present research was to perform a systematic review to assess the antimicrobial effectiveness of commercially available bioceramic root canal sealers against Enterococcus faecalis in accordance with the PRISMA statement. A literature search was made in four electronic databases: MEDLINE, ClinicalKey, ScienceDirect and EBSCO. Were included in vitro studies, published in English, between 2015 and 2019. The evaluation of the selected studies was performed by two authors, independently. Four studies were included in the review. EndoSequenceTM (Brasseler USA, Savannah, GA) also available on the market as TotalFillTM (Brasseler USA, Savannah, GA) and Bi...