?-lactamase gene blaSHV detected in bacteria isolated from retail sushi inKampar, Malaysia (original) (raw)

Biomedical Research-tokyo, 2014

Abstract

Increasing prevalence of multi-antibiotic resistant bacteria in humans is a major concern for public health. The widespread use of antimicrobial agents in plant production, animals and food production has created a reservoir of resistant bacteria with resistance genes that can be transmitted to humans through foodborne route. Hence, the priority of this study was to isolate and characterize multi-antibiotic resistant bacteria from retail sushi in Kampar. Twenty sushi samples consisting of egg, shrimp, cucumber, raw salmon, and fish roe were sampled. A total of 22 bacteria colonies were isolated on nutrient agar supplemented with ampicillin. Gram staining, biochemical tests and Kirby-Bauer antibiotic susceptibility tests were carried out to investigate characteristics of bacterial isolates. From the 22 bacterial isolates, 45% (10/22) were found to be multi-antibiotic resistant. All bacterial isolates were then cultured overnight with ampicillin-supplemented nutrient broth and total D...

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