BADAWCZA I DYDAKTYCZNA the effect of dna isolation methods on genetic similarity of winter wheat ( Triticum aestivum L . ) lines using raPd-Pcr techniques (original) (raw)

The aim of this study was to compare the effect of 4 DNA isolation methods on the results of analyses using molecular markers of Random Amplified Polymorphic DNA – Polymerase Chain Reaction (RAPDPCR) in winter wheat lines. DNA was isolated using the method proposed by Thomson and Henry, the column method with a Quiagen DNeasy Plant Kit, a Genomic Mini AX Plant Kit by A&A Biotechnology and the Maxwell® 16 LEV Plant DNA Kit by Promega. After isolation RAPD–PCR analyses were carried out. The molecular study was an evaluation indicator of the DNA isolation methods. The best isolation method was that using the Quiagen DNeasy Plant Kit. Isolation using this kit provided the best DNA quality and the greatest legibility of electropherograms. However, it is the most timeand cost-intensive method of DNA isolation, which limits its applicability at a large number of tested genotypes. In terms of electrophoretic image quality good DNA isolation was provided by the method using a Genomic Mini AX...

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