DNA Breaks, Chromosomal Aberrations And Cell Inactivation Induced By K Ionization Events In DNA (original) (raw)

AIP Conference Proceedings, 2003

Abstract

It is often thought that unrepaired or misrepaired complex DNA double-strand breaks are responsible for cell inactivation and chromosomal aberrations. Here the role of inner-shell (core) ionizations in DNA atoms is studied. Ultrasoft X-rays from LURE synchrotron radiation have been used to produce core events which mimic the ones induced by ionizing radiations. By tuning the X-ray energy below and above the carbon K-threshold, it is possible to achieve a two-fold increase of the number of core-ionizations in DNA for a same dose in cell. Cell survival, DNA double-strand breaks and chromosomal aberrations have thus been studied, at three energies iso-attenuated in biological samples:~ 250, 350 and 800 eV. The relative biological efficiencies of a given dose in cells to induce cell inactivation and chromosome aberrations appear governed by the yields of core events in DNA. On the other hand, the lethal and DNA-breaking efficiencies of core events in DNA appear strongly correlated.

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