Relative expression of genes involved in fructan metabolism in the stem (original) (raw)
2017
Abstract
<p>The expression of four fructosyltransferase (1-FFTA, 1-FFTB, 1-SST and 6-SFT) and four fructan exohydrolase (1-FEHw1, 1-FEHw2, 1-FEHw3 and 6-FEH) genes was determined in the stems from d0 (anthesis) to 21 days after anthesis (d21), in ‘LE 2384’ and ‘Fontagro 69’ genotypes, grown under water stress (WS) and full irrigation (FI) conditions. Levels of expression were measured by qRT-PCR using primers displayed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0177667#pone.0177667.t002" target="_blank">Table 2</a>, and the relative expression calculated by 2<sup>–ΔΔCt</sup> method described in Material and Methods. Vertical lines represent the mean ± SE of three biological replicates, significant differences at P< 0.05*, P<0.001, ** and P<0.0001***.</p
Alejandra Yáñez hasn't uploaded this paper.
Let Alejandra know you want this paper to be uploaded.
Ask for this paper to be uploaded.