Evaluation of Antimicrobial Activity of Sida cordifolia Leaf Extract (original) (raw)
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Phytochemical screening and Antibacterial activity of Sida cordifolia L. (Malvaceae) leaf extract
Phytochemical screening and antibacterial investigation was made in leaf extracts of Sida cordifolia L. The plant materials were collected and shade dried. The ethanol and methanol extracts were prepared using soxhlet apparatus. Ethanol and methanol extracts revealed the presence of alkaloids, glycosides, carbohydrates, flavonoids, phenols, saponins and tannins. The antibacterial activity was studied by using agar-disc diffusion method. The results showed that ethanol and methanol extracts inhibit the growth, in selected organisms. The higher number of zone inhibition were found on the ethanol extract showed activity against virulent bacteria such as Escherichia fecalis, Pseudomonas aeroginosa. The 11 mm of zone of inhibition found at 75 mL/disc concentration, and methanol extract showed that the high inhibitory zone towards, Escherichia coli (8.53 mm) at 75 mL/disc concentration no inhibitions is other two extracts.
EVALUATION OF ANTIMICROBIAL ACTIVITY OF LEAF AND STEM EXTRACTS OF SIDDA MEDICINAL PLANT SIDA CORDATA
TJPRC, 2013
Successive solvent extract viz., petroleum ether, chloroform, ethyl acetate, ethanol and water extracts of leaf and stem of Sida cordata was evaluated for antimicrobial activity, against bacterial pathogens E.coli, S.aureus, Pseudomonas aeruginosa and Lactobacillus and two fungal strains Candida albicans, and Aspergillus niger by agar-well diffusion/broth dilution / Resazurin microtiter method. MIC values for the aqueous extract of leaf varied from 0.187-1.5mg/ml for bacterial isolates, comparatively the ethanol leaf extract showed higher MIC values (0.375-3mg/ml) for all the tested bacteria. Chloroform, Ethyl acetate and Petroleum ether extracts did not show any significant activity. Similarly the antibacterial activity in the different extracts of the stem was comparatively less significant. Increased zone of inhibition was observed with increasing aqueous leaf extract, highest inhibition was with the highest concentration against S.aureus, E.coli and Lactobacillus compared to the inhibition effects on these organisms by Ampicillin used as control, indicating that the active principle responsible for antibacterial activity is more soluble in water. The extracts had no inhibitory effect on fungal isolates. The above findings suggest that Sida cordata is scientifically validate the use of this plant in the Sidda medicine. Further isolation and characterization of the active principle responsible for the antibacterial activity may be an alternate source for antibiotics.
evaluation of sida cordata leaf and stem extract for its anti microbial activity
Successive solvent extract viz., petroleum ether, chloroform, ethyl acetate, ethanol and water extracts of leaf and stem of Sida cordata was evaluated for antimicrobial activity, against bacterial pathogens E.coli, S.aureus, Pseudomonas aeruginosa and Lactobacillus and two fungal strains Candida albicans, and Aspergillus niger by agar-well diffusion/broth dilution / Resazurin microtiter method. MIC values for the aqueous extract of leaf varied from 0.187-1.5mg/ml for bacterial isolates, comparatively the ethanol leaf extract showed higher MIC values (0.375-3mg/ml) for all the tested bacteria. Chloroform, Ethyl acetate and Petroleum ether extracts did not show any significant activity. Similarly the antibacterial activity in the different extracts of the stem was comparatively less significant. Increased zone of inhibition was observed with increasing aqueous leaf extract, highest inhibition was with the highest concentration against S.aureus, E.coli and Lactobacillus compared to the inhibition effects on these organisms by Ampicillin used as control, indicating that the active principle responsible for antibacterial activity is more soluble in water. The extracts had no inhibitory effect on fungal isolates. The above findings suggest that Sida cordata is scientifically validate the use of this plant in the Sidda medicine. Further isolation and characterization of the active principle responsible for the antibacterial activity may be an alternate source for antibiotics.
Antimicrobial spectrum ofAlchornea cordifolia leaf extract
Phytotherapy Research, 1999
Objective: The objective of the study was to evaluate in-vitro efficacy of antibacterial, and antifungal activities of chloroform extract of leaf of Sida cordifolia L. against some human pathogenic bacterial and fungal strains. S. cordifolia (Malvaceae) is used as anti-rheumatic, analgesic, antipyretic, anti-asthmatic, nasal decongestant, antiviral, laxative, diuretic, aphrodisiac, hypoglycemic, hepatoprotective and in the treatment of Parkinson disease. Methodology: Chloroform extract of S.cordifolia was used for antimicrobial screening. Antibacterial activity was tested against pathogenic bacterial strains Eschericha coli and Staphylococcus aureus. Also antifungal activity was tested against one human pathogenic fungal strain i.e. Candida albicans. Antibacterial and antifungal activities of S. cordifolia extract was carried out by using disc diffusion method. Results: Chloroform extract of S. cordifolia showed the largest zone of inhibition (10mm) against S. aureus at 50µgm/mL and no zone of inhibition against E.coli at 50µgm/ml. Chloroform extract has shown more potent antibacterial activity against S.aureus. Chloroform extract was
2016
Sida cordifolia(Linn) is a plant belonging to the family Malvaceae. The present study investigates the phytochemistry, characterization and antibacterial activity of ethanolic extract of Sida cordifolia(Linn).The sample was collected from the Khallikote University campus. The collected plant material was shade dried and pulverized. The plant material was studied for phytochemistry, spectroscopic analysis i.e., H NMR, C NMR, IR, Mass and antibacterial activity. The phytochemical analysis indicates the presence of flavone and glucoside in the plant. The present study provides evidence that the ethanolic extract of Sida cordifolia(Linn) contains bioactive compounds that might make the plant a novel anti V. cholerae O1drug.
Phytopharmacological evaluation of ethanol extract of Sida cordifolia L. roots
Objective: The present study was carried out to investigate the phytochemical screening (group determination) and selected pharmacological activities (antioxidant, antimicrobial and analgesic activity) of the plant Sida cordifolia Linn. For these purposes 80% concentrated ethanolic extract of the roots was used. Methods: To identify the chemical constituents of plant extract standard procedures were followed. In phytochemical screening the crude extract was tested for the presence of different chemical groups like reducing sugar, tannins, saponins, steroids, flavonoids, gums, alkaloids and glycosides. The antioxidant property of ethanolic extract of S. cordifolia was assessed by DPPH free radical scavenging activity. Analgesic activity of the extract was tested using the model of acetic acid induced writhing in mice. Diclofenac sodium is used as reference standard drug for the analgesic activity test. Antibacterial activity of plant extract was carried out using disc diffusion method with five pathogenic bacteria comparison with kanamycin as a standard. Results: Phytochemical analysis of the ethanolic extract of the roots of S. cordifolia indicates the presence of reducing sugar, alkaloids, steroids and saponins. In DPPH scavenging assay the IC50 value was found 50µg/ml which was not comparable to the standard ascorbic acid. The crude extract produced 44.30% inhibition of writhing at the dose of 500 mg/kg body weight which is statistically significant (p>0.001). The in vitro antimicrobial activity of the ethanol extract of the roots of S. cordifolia showed no antimicrobial activity against five types of microorganisms. The experiment was conducted only with five species of bacteria as test species which not at all indicate the total inactivity against micro-organisms. Conclusions: The obtained results provide a support for the use of this plant in traditional medicine but further pharmacological studies are required.
Applied Biochemistry and Biotechnology, 2022
Sida cordata (Burm.f.) Borss.Waalk. is a perennial prostrate herb belonging to the family Malvaceae found in the tropical and subtropical plains of India, Sri Lanka and Pakistan. It is one of the most essential plants in Ayurveda and Siddha system of traditional medicine and is used to treat various diseases and ailments. The present study is carried out to investigate the preliminary phytochemicals and bioactive compounds of leaf extracts by GC–MS. GC–MS analysis of leaf extracts revealed the presence of 50 compounds in all four solvents. Petroleum ether extract showed the presence of 18 compounds, whereas ethanol extract showed 12 compounds; chloroform and acetone extracts showed the presence of 10 compounds each. Phytol is the most signifcant compound in petroleum ether (28.72%), ethanol (32.99%), and acetone (41.61%) extracts. Whereas in chloroform extract, 1,2-benzenedicarboxylic acid (49.07%) is the most signifcant bioactive compound having antioxidant, antifouling and antimicrobial properties
PRELIMINARY PHYTOCHEMICAL EVALUATION OF ROOT OF SIDA CORDIFOLIA LINN.
Sida cordifolia Linn. (Malvaceae) is a widely used drug for various ailments in Ayurvedic medicine. It is considered as Bala in Ayurvedic classical text books. Phytochemical evaluation of a drug helps in its standardization and to justify its use in various formulations. In this study, preliminary physical and phytochemical evaluation of root of Sida cordifolia Linn including High Performance Thin Layer Chromatography was done. Qualitative analysis showed the presence of steroid, alkaloid, saponin, tannin and flavonoids.
Investigation of pharmacognostical and preliminary phytochemical characters of sida cordifolia
International Journal of Research in Pharmacy and Pharmaceutical Sciences , 2019
Objective: To study detailed pharmacognostic profile and preliminary phytochemical investigation of leaf of Sida cordifolia (Malvaceae). In Ayurvedic system of medicine it is used as anti-rheumatic, analgesic, antipyretic, anti-asthmatic, nasal decongestant, antiviral, laxative, diuretic, aphrodisiac, hypoglycemic, hepatoprotective and in the treatment of parkinson disease. Methods: Leaf, of Sida cordifolia was studied by Macroscopical, Microscopical, Physiochemical, Phytochemical analysis of powder of the plant and other methods for standardization recommended by WHO. Results: Macroscopically, the leaves are simple. 2.5-7 cm long and 2.5-5 cm broad, with 7-9 veins, heart shaped, serrate and truncate, acute apex, toothed margin, reticulate venation, soft, dark green colour, characteristic odour and bitter taste. Microscopically, the leaf showed. thin cuticle on both upper and lower epidermis with stellate trichomes. Upper epidermis single cell-layered, thick, and is composed of oval to oblong cells. Mesophyll tissue is consisting of compactly arranged, rectangular, elongated palisade cells followed by rounded to oval shaped, loosely arranged spongy cells. In the midrib region, the upper epidermis is very thinly cuticularised with different types of trichomes. Vascular bundle sheath is distinct and composed of sclerenchymatous cells. Xylem elements are radially elongated and followed by phloem elements. A few calcium oxalate crystals are present in this layer. Stomata is anisocytic type and some places occur paracytic stomata. The investigations also included leaf surface data; quantitative leaf microscopy. Preliminary phytochemical screening revealed the presence of reducing sugar, alkaloids, saponin and steroids. Conclusions: The results of the study can serve as a valuable source of information and provide suitable standards for identification of this plant material in future investigations.
Phytochemical Standardisation and Antimicrobial Effect of Sida rhombifolia Linn. Aerial Parts
Journal of Ayurveda Medical Sciences, 2018
Introduction: Sida rhombifolia belonging to family Malvaceae is widely used in Ayurvedic practice for the treatment of infectious diseases, fever, diarrhea and diuretic. Several bioactive compounds are reported from the plant having medicinal activities. Methods: Standardization has been done to ensure the quality and purity of authentic specimen, Phytochemical test was carried out to explore its phyto-constituents. HPTLC fingerprinting profile was also been carried out. Further the ethanolic and chloroform extract of aerial parts was screened for antibacterial and antifungal activity by Agar well diffusion method. Results: The chloroform extract showed significant antibacterial and antifungal activity when compared to the ethanolic extract. Preliminary phytochemical screening revealed the presence of alkaloids, carbohydrates, tannin and coumarins. Distinct spots are also observed in the HPTLC prints. Conclusion: The present investigation provides useful information on antimicrobial activity of chloroform extract of Sida rhombifolia in treatment of various bacterial and fungal infections.