Flower-like ZnO nanostructure based electrochemical DNA biosensor for bacterial meningitis detection (original) (raw)
Related papers
Application of nanostructured ZnO films for electrochemical DNA biosensor
a b s t r a c t Nanostructured zinc oxide (nsZnO) films have been fabricated onto conducting indium-tin-oxide (ITO) coated glass plate, by cathodic electro-deposition to immobilize probe DNA specific to M. tuberculosis via physisorption based on strong electrostatic interactions between positively charged ZnO (isoelectric point = 9.5) and negatively charged DNA to detect its complementary target. Electrochemical studies reveal that the presence of nano-structured ZnO results in increased electro-active surface area for loading of DNA molecules. The DNA-nsZnO/ITO bioelectrode exhibits interesting characteristics such as detection range of 1 × 10 −6 − 1 × 10 −12 M, detection limit of 1 × 10 −12 M (complementary target) and 1 × 10 −13 M (genomic DNA), reusability of about 10 times, response time of 60s and stability of up to 4 months when kept at 4°C.
ZnO nanostructure-based electrochemical biosensor for Trichinella DNA detection
Sensing and Bio-Sensing Research, 2019
ZnO nanostructures significantly develop surface of the electrode and increase a number of adsorption bonds for the DNA immobilization. From morphologies of nanostructures that have been studied in this research, the ZnO nanotubes exhibit the best sensitivity on the immobilization stage. The ZnO nanotubes sensor allows unambiguously to distinguish complementary, noncomplementary, and partially complementary DNA sequences for the Trichinella britovi primers in the analyte, as well as to determine the species of the PCR product.
Zinc Oxide Nanowire Decorated Single-Use Electrodes for Electrochemical DNA Detection
Journal of the American Ceramic Society, 2014
The surfaces of pencil graphite electrodes (PGEs) were decorated with zinc oxide nanowires (ZnO NWs) for the electrochemical detection of nucleic acids. ZnO NWs were synthesized through simple hydrothermal method. PGEs decorated with ZnO NWs (ZnO NW/PGEs) were electrochemically characterized through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) following morphological characterization through transmission (TEM) and scanning electron microscopy (SEM). Enhanced sensor response obtained using ZnO NW/PGEs contrary to the bare PGE (control) samples. Our preliminary results simply reveal the potential of combining ZnO NWs with disposable sensor technology for the electrochemical detection of DNA.
Open Journal of Applied Biosensor, 2014
Herein we report an electrochemical DNA biosensor for the rapid detection of sequence (5' AAT GGA TTT ATC TGC TCT TCG 3') specific for the breast cancer 1 (BRCA1) gene. The proposed electrochemical genosensor is based on short oligonucleotide DNA probe immobilized onto zinc oxide nanowires (ZnONWs) chemically synthesized onto gold electrode via hydrothermal technique. The morphology studies of the ZnONWs, performed by field emission scanning electron microscopy (FESEM), showed that the ZnO nanowires are uniform, highly dense and oriented perpendicularly to the substrate. Recognition event between the DNA probe and the target was investigated by differential pulse voltammetry (DPV) in 0.1 M acetate buffer solution (ABS), pH 7.00; as a result of the hybridization, an oxidation signal was observed at +0.8 V. The influences of pH, target concentration, and non-complimentary DNA on biosensor performance were examined. The proposed DNA biosensor has the ability to detect the target sequence in the range of concentration between 10.0 and 100.0 µM with a detection limit of 3.32 µM. The experimental results demonstrated that the prepared ZnONWs/Au electrodes are suitable platform for the immobilization of DNA.
Colloids and Surfaces B: Biointerfaces, 2011
In this study, ZnO nanoparticles (ZNP) of approximately 30 nm in size were synthesized by the hydrothermal method and characterized by X-ray diffraction (XRD), Braun-Emmet-Teller (BET) N 2 adsorption analysis and transmission electron microscopy (TEM). ZnO nanoparticles enriched with poly(vinylferrocenium) (PVF + ) modified single-use graphite electrodes were then developed for the electrochemical monitoring of nucleic acid hybridization related to the Hepatitis B Virus (HBV). Firstly, the surfaces of polymer modified and polymer-ZnO nanoparticle modified single-use pencil graphite electrodes (PGEs) were characterized using scanning electron microscopy (SEM). The electrochemical behavior of these electrodes was also investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Subsequently, the polymer-ZnO nanoparticle modified PGEs were evaluated for the electrochemical detection of DNA based on the changes at the guanine oxidation signals. Various modifications in DNA oligonucleotides and probe concentrations were examined in order to optimize the electrochemical signals that were generated by means of nucleic acid hybridization. After the optimization studies, the sequence-selective DNA hybridization was investigated in the case of a complementary amino linked probe (target), or noncomplementary (NC) sequences, or target and mismatch (MM) mixture in the ratio of (1:1).
Stepwise Functionalization of ZnO Nanotips with DNA
Langmuir, 2009
A surface functionalization methodology for the development of ZnO nanotips biosensors that can be integrated with microelectronics was developed. Two types of long chain carboxylic acids linkers were employed for the functionalization of 0.5 µm thick MOCVD-grown ZnO nanotip films with single-stranded DNA (ssDNA), followed by hybridization with complementary ssDNA tagged with fluorescein. The ZnO functionalization strategy was developed for the fabrication of ZnO nanotips-linker-biomolecule films integrated with bulk acoustic wave (BAW) biosensors, and it involved three main steps. First, 16-(2-pyridyldithiol)hexadecanoic acid or N-(15-carboxypentadecanoyloxy) succinimide, both bifunctional C16 carboxylic acids, were bound to ZnO nanotip films through the COOH group, leaving at the opposite end of the alkyl chain a thiol group protected as a 2-pyridyl disulfide, or a carboxylic group protected as a N-succinimide, respectively. In the second step, ssDNA was covalently linked to each type of ZnO-linker film: the 2-pyridyl disulfide end group was substituted with 16 bases 5′-thiol-modified DNA (SH-ssDNA), and the N-succinimide ester end group was substituted with 16 bases 5′-amino-modified DNA (NH 2 -ssDNA). In the third step, the DNA-functionalized ZnO nanotip films were hybridized with complementary 5′-fluorescein ssDNA. The surfacemodified ZnO nanotip films were characterized after each step by FT-IR-ATR, fluorescence emission spectroscopy, and fluorescence microscopy. This functionalization approach allows sequential reactions on the surface and, in principle, can be extended to numerous other molecules and biomolecules. D Langmuir, Vol. xx, No. x, XXXX Taratula et al. LA8026946 Figure 7. Route B: Fluorescence emission spectra of ZnO-N modified with ssDNA (black line) and ZnO-N with Fl-dsDNA (red line). λ ex ) 490 nm. 25
2018
A specific double-stranded DNA sensing system is of great interest for diagnostic and other biomedical applications. Zinc finger domains, which recognize double-stranded DNA, can be engineered to form custom DNA-binding proteins for recognition of specific DNA sequences. As a proof of concept, a sequence-enabled reassembly of TEM-1 β- lactamase system (SEER-LAC) was previously demonstrated to develop zinc finger protein (ZFP) arrays for the detection of a double-stranded bacterial DNA sequence. Here, we implemented the SEER-LAC system to demonstrate the direct detection of pathogenspecific DNA sequences present in E. coli O157:H7 on the lab-on-a chip. ZFPs customdesigned to detect shiga toxin in E. coli O157:H7 were immobilized on the cyclic olefin copolymer (COC) chip, which can function as a non-PCR based molecular diagnostic. Pathogen-specific double-stranded DNA was directly detected by engineered ZFPs immobilized on the COC chip, providing a detection limit of 10 fmole of targe...
Small, 2009
In this work, a simple but sensitive electrochemical DNA biosensor for nucleic acid detection was developed by taking advantage of exonuclease (Exo) I-assisted cleavage for background reduction and zirconia-reduced graphene oxide-thionine (ZrO 2-rGO-Thi) nanocomposite for integral DNA recognition, signal amplification, and reporting. The ZrO 2-rGO nanocomposite was obtained by a one-step hydrothermal synthesis method. Then, thionine was adsorbed onto the rGO surface, via π-π stacking, as an excellent electrochemical probe. The biosensor fabrication is very simple, with probe DNA immobilization and hybridization recognition with the target nucleic acid. Then, the ZrO 2-rGO-Thi nanocomposite was captured onto an electrode via the multicoordinative interaction of ZrO 2 with the phosphate group on the DNA skeleton. The adsorbed abundant thionine molecules onto the ZrO 2-rGO nanocomposite facilitated an amplified electrochemical response related with the target DNA. Since upon the interaction of the ZrO 2-rGO-Thi nanocomposite with the probe DNA an immobilized electrode may also occur, an Exo I-assisted cleavage was combined to remove the unhybridized probe DNA for background reduction. With the current proposed strategy, the target DNA related with P53 gene could be sensitively assayed, with a wide linear detection range from 100 fM to 10 nM and an attractive low detection limit of 24 fM. Also, the developed DNA biosensor could differentiate the mismatched targets from complementary target DNA. Therefore, it offers a simple but effective biosensor fabrication strategy and is anticipated to show potential for applications in bioanalysis and medical diagnosis.
Journal of Nanomaterials
The study is aimed at investigating the stability of electrochemical and biosensing properties of ZnO nanorod-based platinum screen-printed electrodes (SPEs) applied for detection of bacterial pathogens. The platinum SPEs were designed and patterned according to standard photolithography and lift-off process on a silicon wafer. ZnO nanorods (NRs) were grown on the platinum working electrode by the hydrothermal method, whereas Salmonella polyclonal antibodies were selected and immobilized onto ZnO NR surface via a crosslinking process. Morphological and structural characteristics of ZnO NRs were investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The results showed that the ZnO NRs were grown vertically on platinum electrodes with a diameter around 20-200 nm and a length of 5-7 μm. These modified electrodes were applied for detection of Salmonella enteritidis at a concentration of 103 cfu/mL by electrochemical measu...