Assessment of metals bioaccumulation and bioavailability in mussels Mytilus galloprovincialis exposed to outfalls pollution in coastal areas of Casablanca (original) (raw)
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Oceanologia, 2015
The aim of the present work is to broaden our knowledge on the variability of trace metals in mussel tissues, focusing on seasonal fluctuations in the three different sampling sites of Algerian west coast (Oran Harbor (S1), Ain Defla (S2) and Hadjaj (S3)). For this purpose, the bioavailability (metal indices) and bioaccumulation (metal concentrations in soft tissues) of heavy metals (Zn, Cu, Pb, and Cd), and the physiological characteristics (e.g. biological indices such as condition index (CI)) of mussels Mytilus galloprovincialis have been assessed and related to seasons and sites. In S1, the highest levels of metal concentrations and indices were obtained in mussels sampled in winter for Zn, Cu and Cd, but in summer for Pb. The biological indices significantly decreased in winter. In S2, the levels of concentrations and indices of all metals varied whatever the seasons, excepting in summer where the values were the lowest. In summer Peer review under the responsibility of Institute of Oceanology of the Polish Academy of Sciences. § We thank Ms. Nathalie Bendbiko and Ms. Catinca Birna (University Agency of Francophony (AUF-Paris)) for their support through the granting of the Doctoral scholarship.
Environmental Science and Pollution Research, 2010
Purpose The aim of this study was to compare the level of metal contamination in two bays in the middle part of the Eastern Adriatic coastal zone in Croatia using the gills of mussels Mytilus galloprovincialis as indicator tissue. Despite the existing sources of contamination, previous studies with caged mussels only indicated moderate metal contamination of the Kaštela Bay, contrary to the Trogir Bay in which marina and shipyard present a probable source of Cu- and Zn-contamination. Methods The measurements of metallothioneins (MTs) and metals that induce MT synthesis (Cu, Zn, and Cd) were performed in the heat-treated gill cytosol and total proteins (TPs) in the untreated gill cytosol. MTs were determined by differential pulse voltammetry, Cu and Zn by flame atomic absorption spectrometry (AAS), Cd by graphite furnace AAS, and TPs by Bradford spectrophotometric procedure. Results The results collected in four sampling campaigns (autumn periods from 2001 to 2004) indicated that MT levels in mussel gills (expressed on dry mass basis 2.3 ± 0.3 mg g−1) were comparable with basal levels reported in the literature (2.5 ± 0.8 mg g−1). Observed interindividual, temporal, and spatial MT variability could be associated with different confounding factors, such as the time of sampling, total protein concentration, and mussel size rather than cytosolic levels of Cu and Zn. Metal levels, expressed on wet mass basis, in the heat-treated gill cytosol ranged from 1.33 to 11.31 μg g−1 for Zn, from 0.72 to 2.96 μg g−1 for Cu, and from 0.036 to 0.100 μg g−1 for Cd. The highest Zn level was measured at Vranjic (Kaštela Bay)—the site influenced by untreated domestic wastewater, while somewhat increased Zn and the highest Cu levels were found at marina and shipyard locations (Trogir Bay). The highest Cd level was measured at Inavinil (Kaštela Bay). Conclusions The observed association of gill MT levels with several biotic and abiotic factors limits its use as the biomarker of low-level metal exposure. Therefore, the use of the metal concentrations in the heat-treated gill cytosol of Mediterranean mussels should be considered for the assessment of the low-level metal contamination of coastal marine areas.
2020
In the present work, both seawater and mussels (M. galloprovincialis) were collected monthly from the mussel farm located along the coastline of Al Hoceima (Morocco) and their heavy metal concentrations (Fe, Cu, Ni) were determined using inductively coupled plasma – optical emission spectrometry (ICP-OES). The trace metals found in the mussel tissues are much higher than those found in the environment. The order of the abundance of metals in the mussels is Fe > Cu > Ni. The pattern of the temporal variation of mussel trace metal levels was similar among stations with higher values during the months of winter season and lower during the months of summer period. Heavy metals uptake is dependent on both environmental and biological factors. Results obtained in this study for Fe, Cu and Ni, indicate that the species under investigation poses no health hazard to consumers because its trace metal contents remain within the permissible range established by various international guide...
Marine Pollution Bulletin, 2008
The Western Scheldt river and estuary is known to be highly polluted as it receives industrial, agricultural and domestic effluents from one of the most populated and industrialised zones in Europe. Aquatic organisms are exposed to pollutants, specifically metals that are present in different environmental phases, e.g. dissolved, suspended material or sediment phases. The objective of this study was to study the relationship that exists between environmental metal levels, the degree of metal uptake by aquatic organisms with the concomitant biological responses. For this purpose the bivalve mollusk, Mytilus edulis, was selected as bioaccumulation indicator organism. Environmental (water and sediment) and mussel samples were collected during the late winter (March 2000) from sampling sites in the Scheldt estuary. Sites were selected to represent a salinity gradient from the mouth of the estuary to the furthest distribution area of mussels upstream in the system. Together with standard water quality parameters (e.g. salinity, dissolved oxygen, dissolved organic carbon, etc.) concentrations of twelve metals were analysed in the water (dissolved and suspended matter) and sediments. Levels of these metals were also measured in the soft tissue of M. edulis, together with concomitant biomarker responses in resident mussel populations at each site. The biomarkers that were included in this study were condition index, scope for growth, survival in air, cell membrane stability, DNA damage, and metallothioneins. Data were subjected to multivariate statistical analysis. The physico-chemical parameters and metals in the environmental samples clustered the sites to reflect the distribution based on the salinity gradient. Bioaccumulation results revealed increased metal uptake along a pollution gradient with the highest metal bioaccumulation occurring at the upstream most sites and therefore closest to the in the industrial activities. However, the biomarker responses clustered the sites in a manner that reflected the influence of combination of internal exposure (bioaccumulation) and external exposure (physico-chemical conditions). These differences in biomarker responses clearly demonstrated were attributed to abiotic factors other than metal pollution alone e.g. localized short-term increases in increased suspended sediment concentrations and decreased dissolved oxygen concentrations.
Journal of Applied Sciences Research
The essential (Cu and Zn) and the non-essential (Pb and Ni) trace metal concentrations were studied in the mussel Donax trunculus from sites exposed to different urban and industrial pollution. Results have indicated that the unpolluted location has recorded the lowest metal levels. However, the concentrations of Pb, Ni, and Zn were significantly higher in site exposed to industrial pollution compared to the other sites. Males have accumulated more nickel than females, from the industrial polluted site, whereas females have higher Cu and Zn compared to males, from the relatively clean site. This study suggests that D. trunculus might be used for trace metal monitoring in Annaba estuaries, and it is not recommended for human consumption in the sites close to Annaba city. Trace metals accumulation pattern in Donax is not only under metal enrichment effect, but it also affected by sex differences.
2012
A study was conducted to determine the contamination of Mediterranean mussels (Mytilus galloprovincialis) by some essential and non-essential heavy metals collected from the large Oran Harbour (Oran Bay, Algerian west coast) in November (fall) 2009 and 2010. A total of fifty individuals of mussels, Mytillus galloprovincialis were sampled and metals such as Zinc (Zn), Copper (Cu), Lead (Pb) and Cadmium (Cd) concentration were determined using a flame atomic absorption spectrophotometer equipped with a graphite furnace. The observed mean concentration of Zn, Cu, Pb and Cd in the soft tissues of mussels was respectively, 95.24±1.85, 5.27±0.18, 16.74±1.74 and 0.66±0.04 μg/g dry weight in samples of 2009 and 83.35±2.19, 1.15±0.12, 11.65±0.55 and 0.68±0.04 μg/g dry weight in those of 2010. These concentrations were relatively low compared to those found in mussels of other parts of the world and below certified safety guidelines except the Pb concentrations which exceeded the permissible ...
Science of The Total Environment, 2000
The occurrence of changes in flesh and shell weights and in other biometric parameters of mussels, Mytilus gallopro¨incialis, has been related to different metal levels found in their soft tissues. The effects of clean and Zn-polluted environments and laboratory experiments where Zn-polluted mussels were exposed to sublethal concentrations of Zn, Cu and Cd were investigated. Zinc-polluted mussel shell weights increased significantly after a 51-day depuration period. Exposure of Zn-polluted mussels to Zn or Cd, however, caused a slightly reduced shell growth in comparison with depurating mussel Cu-exposures not causing any reduction in growth. Apart from metal concentrations, metalrshell weight indices have been used to assess metal bioavailability. Metal concentrations recorded in the soft tissues of depurating mussels increased without a source of 'extra' metals, while the Znrshell-weight index was reduced, as expected from a depuration process, Cu and Cdrshell-weight indices remaining constant. Experimental exposure to Zn, Cu and Cd caused augmented values of Zn, Cu and Cdrshell-Ž weight indices, respectively. These different findings were attributed to changes in flesh weight related with gamete . spawning that would produce inconsistent estimates of whole metal concentration in soft tissues. Since changes in the tissue composition and in growth rates do not affect ZnrCu ratios and metalrshell-weight indices, these parameters are proposed as reliable indices of metal bioavailability for 'Mussel-Watch' monitoring programmes. The most sensitive parameter is the metalrshell-weight index, which is, therefore, highly recommended to be used in 'Mussel-Watch' monitoring programmes in order to determine metal bioavailability in seawaters. ᮊ M. Soto 0048-9697r00r$ -see front matter ᮊ 2000 Elsevier Science B.V. All rights reserved. Ž . PII: S 0 0 4 8 -9 6 9 7 9 9 0 0 4 8 9 -1 ( ) M. Soto et al. r The Science of the Total En¨ironment 247 2000 175᎐187 176
Journal of Sea Research, 2006
Bioaccumulation of metal within an organism results from interactions between physiological factors (growth, weight loss, absorption and accumulation), chemical factors (metal concentration, speciation and bioavailability) and environmental factors (temperature and food concentration). To account for such interactions in the mussel Mytilus galloprovincialis, we combined bioaccumulation and Dynamic Energy Budget models. Field experiments were conducted to measure uptake and elimination kinetics for two metals (Hg and Pb) in three Mediterranean sites with differences in contamination levels and to calibrate the models. Metal uptake from water and from food was considered separately. Metal elimination resulted from reproduction and/or from direct excretion. Contributions of physiological variables, such as body size and tissue composition were quantified. By combining environmental and biological data, the model provided an efficient bio-monitoring tool which can be applied to various coastal environments. An application to the French bio-integrator network (RINBIO) was carried out through inverse analysis and enabled to assess the real level of contamination in water on the basis of contamination measured in mussels.
Environmental Monitoring and Assessment, 2016
In the present work, concentration of eight metals (Cd, Cr, Cu, Fe, Mn, Ni, Pb, Zn) was determined in the sediments, transplanted and native mussels (Mytilus galloprovincialis). The study was conducted in Turkish marinas, shipyards and shipbreaking yards. The effect of metal pollution was evaluated by determining the levels of metallothionein (MT) in the mussels. The extent of contamination for each single metal was assessed by using geoaccumulation index (Igeo) and enrichment factor (EF). Whereas, to evaluate the overall metal pollution and effect, pollution load index (PLI), modified contamination degree (mCd), potential toxicity response index (RI), mean ERM quotient (m-ERM-Q) and mean PEL quotient (m-PEL-Q) were calculated. The influence of different background values on the calculations was discussed. The results indicated a significant metal pollution caused by Cu, Pb and Zn especially in shipyard and shipbreaking sites. Higher concentrations of MT were observed in the ship/breaking yard samples after the transplantation.