HPTLC analysis and anti-inϐlammatory activity of Plectranthus amboinicus (Lour.) leaves (original) (raw)
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Phyto-Physicochemical Investigation of Leaves of Plectranthus amboinicus (Lour) Spreng
Pharmacognosy Journal, 2010
Plectranthus amboinicus (Lour) Spreng belongs to family Lamiaceae and known as country borage in English. It is large succulent aromatic perennial herb, shrubby below, hispidly villous or tomentose. It is found throughout India, Sri Lanka and Moluccas. The leaves of the plant are bitter, acrid and were being widely used traditionally for various purposes. The present study was therefore carried out to provide requisite pharmacognostic details about the leaf. The leaf was characterized for its physico-chemical properties. The shade dried powder of leaves of Plectranthus amboinicus (Lour) Spreng was subjected to successive extraction using the solvents (petroleum ether, chloroform, ethanol and water) in the increasing order of polarity. The preliminary phytochemical analysis and Thin Layer Chromatography has been performed. It was found that the leaves revealed the presence of alkaloids, carbohydrates, glycosides, proteins, amino acids, flavonoids, quinone, tannins, phenolic compounds and terpenoids. The findings will be useful towards establishing pharmacognostic standards for identification, purity, quality and preparation of monograph of the plant.
Ethnopharmacological relevance: Plectranthus amboinicus is used in traditional medicine to treat several diseases and ailments including opportunistic infections associated with HIV/AIDS. However, there is little insight as to the possible toxic effects of this herbal formulation on major organ systems of the body. This study was designed to assess the acute and sub acute toxic effects of the aqueous leaf extract of P. amboinicus on the histology of tissues, biochemical (liver and kidney functions) as well as hematological indices in albino swiss mice and wistar rats respectively. Materials and methods: In this study, phytochemical screening of the aqueous extracts of P. amboinicus was carried out using standard qualitative and quantitative (SPME and GCMS) analytical methods. The extracts were tested for acute toxicity in mice and for sub acute toxicity in Wistar rats according to the OECD guidelines No 425. In acute toxicity test, the dose of 5000 mg/kg was used as the limit dose t...
Plectranthus Amboinicus: A Review on Its Pharmacological And, Pharmacognostical Studies
American Journal of Physiology, Biochemistry and Pharmacology, 2020
In this review, information regarding the phytochemistry, traditional uses, medicinal uses, side effects, and future perspectives of Plectranthus amboinicus are given. Plectranthus amboinicus has been used in herbal medicines for the treatment of various disorders such as asthma, flu, eczema, and cardiovascular disorders. The plant possesses medicinal properties (such as antimicrobial activity, antifungal activity, anti-inflammatory activity, antidiabetic activity, anxiolytic activity, antineoplastic, analgesic, antimalarial, antibiofilm efficacy, diuretic, wound healing activity, skincare, respiratory disorders, and antiplatelet aggregation activity) due to the presence of various important constituents or secondary metabolites such as flavonoids, glycosides, phenols, tannins, and steroids, which have been identified through various spectroscopic methods. The different parts of the plant have used to prepare medicines. Due to the presence of various important constituents, this plant can be used for medicines without side effects.
Plectranthus amboinicus (Lour.) Spreng. is a perennial herb belonging to the family Lamiaceae which occurs naturally throughout the tropics and warm regions of Africa, Asia and Australia. This herb has therapeutic and nutritional properties attributed to its natural phytochemical compounds which are highly valued in the pharmaceutical industry. Besides, it has horticultural properties due to its aromatic nature and essential oil producing capability. It is widely used in folk medicine to treat conditions like cold, asthma, constipation, headache, cough, fever and skin diseases. The leaves of the plant are often eaten raw or used as flavoring agents, or incorporated as ingredients in the preparation of traditional food. The literature survey revealed the occurrence 76 volatiles and 30 non-volatile compounds belonging to different classes of phytochemicals such as monoterpenoids, diterpenoids, triterpenoids, sesquiterpenoids, phenolics, flavonoids, esters, alcohols and aldehydes. Studies have cited numerous pharmacological properties including antimicrobial, antiinflammatory, antitumor, wound healing, anti-epileptic, larvicidal, antioxidant and analgesic activities. Also, it has been found to be effective against respiratory, cardiovascular, oral, skin, digestive and urinary diseases. Yet, scientific validation of many other traditional uses would be appreciated, mainly to discover and authenticate novel bioactive compounds from this herb. This review article provides comprehensive information on the botany, phytochemistry, pharmacology and nutritional importance of P. amboinicus essential oil and its various solvent extracts. This article allows researchers to further explore the further potential of this multi-utility herb for various biomedical applications.
Pharmacognostical, Pharmacological Studies and Traditional uses of Plectranthus Ambonicus: A Review
Research Journal of Pharmacognosy and Phytochemistry , 2019
In this review, information about the Phytochemistry, Traditional uses, medicinal uses, side effects and future prospective of Plectranthus Ambonicus are given. PA was used in herbal medicines for the treatment of various disorders like asthma, flu, eczema and cardiovascular disorders. The plant shows these properties due to the presence of various important constituents or secondary metabolites like flavanoids, glycosides, phenols, tannins, and steroids, etc. which were already identified by various spectroscopic methods. The different parts of the plant were used to prepare medicines. As the plant contain various important constituents and also found effective in traditional system of medicine there is the future scope of this plant so that it can be used to prepare medicines which are effective against diseases without side effects
https://www.ijhsr.org/IJHSR\_Vol.9\_Issue.5\_May2019/IJHSR\_Abstract.045.html, 2019
Plectranthus amboinicus have remarkable properties, which has been investigated since ancient times. Scientific evidence stated that P. amboinicus is capable of cure from cough, chronic asthma, hiccup, bronchitis, helminthiasis, colic, convulsions, and epilepsy. This study was carried out with an objective to investigate the antibacterial potentials of leaves of P. amboinicus. The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on bacterial strains. In the present study, the microbial activity of hydroalcohol extracts of leaves of P. amboinicus was evaluated for potential antimicrobial activity against medically important bacterial and fungal strains. The antimicrobial activity was determined in the extracts using agar well diffusion method. The antibacterial activities of extracts of P. amboinicus were tested against two Gram-negative bacteria-Klebsiella pneumoniae and Shigella flexneri. The findings revealed that P. amboinicus showed inhibition zone on the growth of K. pneumoniae and S. flexneri. It is therefore confirmed as a useful antimicrobial agent. The phytochemical analyses of the plants were carried out. The leaves of P. amboinicus rich in phytochemicals and secondary metabolites such as steroids, tannins, flavonoids, alkaloids etc. which are probably responsible for its medicinal properties. The microbial activity of the P. amboinicus was due to the presence of various secondary metabolites. It therefore suggests that constituent of plant extract could serve as a source of drugs useful in the chemotherapy of enteric diseases. Hence, these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.
The present investigation was intended to evaluate the toxicity of the methanolic leaf extract of a traditionally used plant Plectranthus amboinicus (Lour) Spreng. Plant material was analysed for various pharmacognostical parameters as per WHO guidelines procedure i.e., foreign matter, microscopical sections, loss on drying, water and alcoholic extractive values, Total ash, acid soluble ash, heavy metals, phytochemical analysis and toxicity studies. Acute & Sub acute toxicity of the methanolic extract was evaluated in albino mice (Female) after ingestions of the extract during one day (Acute model) and during 28days (sub acute model).The studies on sub acute toxicity reveals that no mortalities or evidence of adverse effects have been observed in Albino mice following acute oral administration at the highest dose of 2000mg/kg crude extracts of Plectranthus amboinicus (Lour) Spreng. Similarly, in sub-acute toxicity study methanolic extract 200,400 mg/kg body wt of Plectranthus amboinicus did not cause any changes in hematological and biochemical parameters. Studies on histopathological examination of vital organs showed normal architecture suggesting no morphological disturbances. Plectranthus amboinicus (Lour) Spreng can be considered as safe as it did not cause either any lethality or adverse changes in the general behavior in mice.
Antioxidant and Cytotoxic Activities of Plectranthus amboinicus (Lour.) Spreng. Extracts
2013
Objectives: to evaluate the antioxidant and cytotoxic activities of Plectranthus amboinicus, (Lour.) Spreng. Methods: The leaves of Plectranthus amboinicus, (Lour.) Spreng. were extracted using n-hexane, aetylacetate and ethanol by maceration method. The antioxidant activity was tested using DPPH and Beta Carotene-Linoleic Acid methods, and compared to those of BHA (butylated hydroxyanisole), BHT (butylated hydroxytoluene) and quercetin. Cytotoxic assay was performed on MCF7 breast cancer cell line, using MTT [3(4,5-dimetiltiazol-2-il)-2,5 difeniltetrazolium bromide) assay. Results: The aetylacetate and ethanolic extracts of Plectranthus amboinicus, (Lour.) Spreng. leaves were found to exhibit antioxidant activity with IC50 value 350.74 µg/mL and 281.26 µg/mL by DPPH method. The ethanolic and aetylacetate extracts showed a high antioxidant activity by s-Carotene-Linoleic Acid Method. N-hexane and aetylacetate extracts showed potent cytotoxic effect on MCF7 with IC50 63.644 µg/mL, 7....
[Plectranthus amboinicus (Lour.) Spreng.]-Pharmacological and Ethnomedicinal Uses – A Review
2021
or Karpooravalli [Plectranthus amboinicus (Lour.) Spreng. ] is an aromatic, succulent perennial herb commonly cultivated in gardens throughout India. The drug possess Kaṭu, Tikta rasa, laghu, ūkṣa, tῑkṣ a gu a, uṣ a vῑrya and kaṭu ip k and is K ph tahara in action. It is indicated in the conditions like Ādhm , Ag im d , Ajῑr a, Aruci, Atis , Graha i, Gulma, Hikk , Hṛdayadaurbalya, Jῑr ś s , K s , Kṛmi, Mūt kṛchra, Mūt og , Mūt śm i, Ś s , Udararoga, Unmada, Viṣūcik . Looking into the pharmacological profile, the drug is scientifically proven to be antimicrobial, antiasthmatic, antiepileptic, antitumorigenic, anti inflammatory, antioxidant, analgesic, antiurolithiatic, anti clastogenic, larvicidal, anti rheumatoid, diuretic, lactogenic, radioprotective, nephroprotective and has wound healing property too. It is widely used traditionally for various ethnomedicinal purposes and is a part of various home remedies in South India, particularly for respiratory conditions.
Pharmacognosy Journal, 2012
Background: Volatile oil, terpenoids, mainly diterpene and polyphenolic compounds including flavonoids and phenolic acids were previously isolated from different Plectranthus species. Plectranthus amboinicus (Lour.) Spreng growing abroad was subjected to phytochemical study resulted in isolation of several flavonoids, also the plant exhibited antioxidant, diuretic, anti-inflammatory, cytotoxic and antimicrobial activities. Materials and Methods: In this study ethyl acetate fraction of Plectranthus amboinicus (Lour.) Spreng leaves growing in Egypt was fractionated and chromatographed on silica gel and sephadex to isolate its phenolic constituents. The isolated compounds were identified using UV, 1 HNMR and 13 CNMR. Total phenolics and tannins content of the leaves, stems and roots of Plectranthus amboinicus (Lour.) Spreng were determined using Folin-Ciocalteau and Folin-Denis reagents, respectively. Phenolic compounds of the stems and roots were identified using UPLC-MS analysis. Leaves, stems and roots of this plant were tested for antioxidant, anti-inflammatory, analgesic, diuretic, cytotoxic and antimicrobial activities. Results: The isolated compounds were identified as 3-methoxy genkwanin, crisimaritin, p-coumaric acid, caffeic acid, taxifolin, rosmarinic acid, apigenin and 5-O-methyl-luteolin. The stems showed the highest concentration of the total polyphenolics followed by the leaves then the roots (9.6, 8.4 and 5.4 mg/g of gallic acid equivalents, respectively), while the roots recorded the highest tannins content followed by the leaves then the stems (126, 90 and 81 µg/g of tannic acid equivalents, respectively). UPLC-MS analysis revealed the presence of caffeic acid, rosmarinic acid, coumaric acid and chrysoeriol in the stems and roots, while luteolin, quercitin and eriodyctiol were detected only in the stems. The different extracts of the three organs exhibited antioxidant, anti-inflammatory, analgesic, diuretic, cytotoxic and antimicrobial activities with variable potency.