Action of anti-bothropic factor isolated from Didelphis marsupialis on renal effects of Bothrops erythromelas venom (original) (raw)

Acute renal failure is the most common complication in the lethal cases caused by snakebites in Brazil. Among the Brazilian venom snakes, Bothrops erythromelas is responsible for the majority of accidents in Northeastern Brazil. Didelphis marsupialis serum could inhibit myonecrotic, hemorrhagic, edematogenic hyperalgesic and lethal effects of envenomation determined by ophidian bites. In the present study, we evaluated the action of the anti-bothropic factor isolated from D. marsupialis on the renal effects promoted by B. erythromelas venom without systemic interference. Isolated kidneys from Wistar rats were perfused with Krebs-Henseleit solution containing 6% bovine serum albumin. We analyzed renal perfusion pressure (PP), renal vascular resistance (RVR), glomerular filtration rate (GFR), urinary flow (UF), and the percentages of sodium and potassium tubular transport (%TNa C , %TK C). The B. erythromelas venom (10 mg mL K1) decreased the PP (ctZ108.71G5.09 mmHg; BEZ65.21G5.6 mmHg*) and RVR (ctZ5.76G0.65 mmHg mL K1 g K1 min K1 ; BEZ3.10G0.45 mmHg mL K1 g K1 min K1 *). On the other hand, the GFR decreased at 60 min (ct 60 Z0.76G0.07 mL g K1 min K1 ; BE 60 Z0.42G0.12 mL g K1 min K1 *) and increased at 120 min (ct 120 Z0.72G0.01 mL g K1 min K1 ; BE 120 Z1.24G0.26 mL g K1 min K1 *). The UF increased significantly when compared with the control group (ctZ0.14G0.01 mL g K1 min K1 ; BEZ0.47G0.08 mL g K1 min K1 *). The venom reduced the %TNa C (ct 90 Z79.18G0.88%; BE 90 Z58.35G4.86%*) and %TK C (ct 90 Z67.20G4.04%; BE 90 Z57.32G5.26%*) The antibothropic factor from D. marsupialis (10 mg mL K1) incubated with B. erythromelas venom (10 mg mL K1) blocked the effects on PP,

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