Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology (original) (raw)

Modification of the protein glycosylation pathway in the methylotrophic yeast Pichia pastoris

Biotechnology Letters, 1998

a-1,2-Mannosidase from Trichoderma reesei was used to modify the N-linked glycosylation pathway of the methylotrophic yeast Pichia pastoris. Expression of foreign influenza glycoproteins with more extensively processed N-linked oligosaccharides was observed when a-1,2-mannosidase was secreted in the culture medium. However, intracellular removal of mannose residues may stimulate mannosyltransferase activity and leads to hyperglycosylation. [3H]Mannose suicide selection or high concentrations of