Modulatory effect of Drosera peltata J.E.Sm on development of metabolic syndrome in tumor bearing mice (original) (raw)
Related papers
2018
10.21276/sajb.2018.6.5.2 Abstract: In the present study was carried out to evaluate the anticancer potential of Drosera peltata J.E.Sm against Ehrlich's Ascites Carcinoma (EAC) induced tumor in mice. The ethanol and aqueous extract of Drosera peltata J.E.Sm. was given orally to mice at the dose of 250, 500 mg/kg body weight for 14 days to EAC bearing mice (4 Groups n=10) and 20mg/kg of 5-Fluorouracil (1 group as standard). Phytoconstituents of both extracts were analyzed through HPTLC methods using plumbagin and quercetin as a standard marker. Treatment caused significant reduction in body weight, packed cell volume (PCV) and viable tumor cell count when compared to the mice of the EAC control group. Restoration of hematological parameters towards normal was also observed. The dose at 250,500mg/kg of ethanol extracts and 500mg/kg of aqueous extract showed significant (p<0.001) result when compared with 250mg/kg of aqueous extract dose. HPTLC study confirmed that presence of p...
Journal of Intercultural Ethnopharmacology, 2012
Aim: In this study, the ethanol and aqueous extracts of Drosera indica L were prepared and cancer induced liver enzyme, lipid profile and hormone changes were studied in mice using the Dalton's lymphoma ascites (DLA) cells. Method: Animals were divided into seven groups as the normal control, DLA control, standard (5FU) and the ethanol and aqueous extracts (250 and 500mg/kg each) of D. indica L + DLA (four groups) were given the respective treatments 24 h after tumor cell inoculation, for 14 days. Result: Both ethanol and aqueous extracts of D. indica L at doses of 250 and 500mg/kg extracts showed significant (p<0.001) effects on the elevated liver enzyme, lipid profile and hormonal changes to normal. Conclusion: The results of the present study demonstrated that both extracts were able to normalize the cancer induced liver enzyme, lipid profile and hormone changes in DLA bearing mice.
Antioxidant potential of Drosera peltata in Dalton Ascites Lymphoma (DAL) bearing mice
International journal of plant based pharmaceuticals, 2023
Cancer is one of the prominent causes of death reported by World Health Organization (WHO). The purpose of this study was to measure the antioxidant status of animals treated with 250 and 500 mg/kg doses of ethanol and aqueous extract of Drosera peltata on Dalton Ascites Lymphoma (DAL) inoculated mice. A total of 70 mice were divided into 7 groups, each group with ten mice. The first group (negative control) received normal food and water for 14 days and was kept under normal conditions. The second group also received normal food and water for 14 days, which was used as a cancer (positive) control. The third group received 5-fluorouracil (20 mg/kg, i.p.) once a day for 14 days. The fourth and fifth group animals received 250 and 500 mg/kg of ethanol extracts of D. peltata (EEDP) whereas the sixth and seventh groups of mice received 250 and 500 mg/kg of aqueous extracts of D. peltata (AEDP), orally for 14 days. All the groups were inoculated with DAL (2 × 10 6 cells/mouse, i.p.) except group I, 24 hours before the commencement of the drug treatment. After the completion of treatment, blood was drawn retro-orbitally and the animals were sacrificed to isolate the liver, lungs, kidneys, and brain for observing tissue antioxidant status. The parameters analyzed were total protein (TP), catalase (CAT), malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (P), and glutathione (GSH) from the tissues apart, and the protein carbonyl content (PCC) also measured from the blood sample. Treatment with EEDP and AEDP significantly lowered the MDA levels from 23 to 10 mmol/ml in the blood, whereas from 28 to 4 nm/g in tissue isolates of the liver, lungs, kidneys, and brain. It also raised the TP, GSH, SOD, CAT, and P levels in the blood and in the tissue samples of the cancer cell line inoculated animals, where their levels were close to those observed in control (negative) group animals. The results proposed that both extracts of D. peltata ameliorated various tissue antioxidant levels in mice with DAL cancer lines comparable to the negative control.
Anticancer activity of Drosera indica L, on Dalton’s Lymphoma Ascites DLA bearing mice
Journal of Intercultural Ethnopharmacology, 2013
Aim: Drosera species are used as vital components in an Ayurvedic preparation called 'Swarnabhasma' (Golden ash) are used for the treatment of different diseases like bronchial asthma, rheumatoid arthritis, diabetes mellitus, nervous disorders In the present study was carried out to evaluate the antitumor potentials against Dalton's lymphoma ascites (DLA)induced tumor. Material and Method: The ethanol and aqueous extract of Drosera indica L., was given orally to mice at the dose of 250, 500 mg/kg body weight for 14 days to DLA bearing mice (4 Groups n=10) and 20mg/kg of 5-Fluorouracil (1 group as standard). Result: Treatment caused significant reduction in body weight, packed cell volume (PCV) and viable tumor cell count when compared to the mice of the DLA control group. Restoration of hematological parameters towards normal was also observed. The dose at 250,500mg/kg of ethanol extracts and 500mg/kg of aqueous extract showed significant (p<0.001) result when compared with 250mg/kg of aqueous extract dose. Conclusion: The results suggest that the ethanol and aqueous extracts of D.indica L., exhibit significant antitumor activity in DLA-bearing mice that is comparable to that of the reference standard, 5-fluorouracil.
In vitro antioxidant and anticancer activity studies on drosera indica L. (Droseraceae)
Advanced pharmaceutical bulletin, 2013
The aim of present in vitro studies was performed to examine the antioxidant and anticancer activities of ethanol and aqueous extracts of Drosera indica L. Different concentrations (5 - 640mcg/ml) of the ethanol (EEDI) and aqueous (AEDI) extracts of D.indica L were used in various antioxidant assay methods such as hydroxyl radicals, DPPH, super oxide radical scavenging activity, chelating ability of ferrous ion, nitric oxide radical inhibition, ABTS and reducing power. Ascorbic acid (AA) was used as the standard antioxidant for the free radical scavenging assays. Dalton's Ascitic Lymphoma (DAL) and Ehrlich Ascitic Carcinoma (EAC) cell lines were used as the in vitro cancer models for the tryphan blue dye and LDH leakage assays, where 5 to 250mcg /ml of both EEDI and AEDI were tested. EEDI showed antioxidant activities with the minimum IC50 values of 34.8±0.43 mcg/ml in scavenging of hydroxyl radical and moreover AEDI showed minimum IC50 values of 94.51±0.84 mcg/ml in Fe(2+)chela...
INDONESIAN JOURNAL OF PHARMACY, 2018
The aim of the present study was to evaluate the antioxidant role of Drosera indica L. in Dalton Ascites lymphoma (DAL) bearing Swiss albino mice. The effect of ethanol and aqueous extracts of D.indica (EEDI and AEDI) were administered at a 250 and 500mg/kg once a day for 14 days, 24h after the inoculation of tumor cell line. After the treatment period, blood was collected from the animals and subsequently the animal were sacrificed for isolation of liver, brain, kidney and lungs for the observation of antioxidant status level. The parameters analyzed were catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), peroxidase (P), total protein (TP) and protein carbonyl content (PCC). Treatment with EEDI and AEDI significantly reduced the levels of MDA and increased the levels of GSH, SOD, CAT, P and TP in cancer induced animal and are similar as that of normal mice. The results suggest that the ethanol and aqueous extract of Drosera indica L. possess significant antioxidant effects in DAL bearing mice.
Biomedicine & Pharmacotherapy, 2016
Among various chronic disorders, cancer and diabetes mellitus are the most common disorders. This study was designed to evaluate the effectiveness of hydroalcoholic extract of Phoenix dactylifera L. leaves (HEPdL) in animal models of type II diabetes in vitro/in vivo and in a human melanoma-derived cell line (IGR-39). A liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was also performed to determine the amount of phenolic and flavonoid compounds in this plant. The physicochemical results by LC-MS/MS analysis of HEPdL showed the presence of 10 phenolic compounds. The in vitro study showed that the extract exhibited a more specific and potent inhibitor of a-glucosidase than a-amylase with an IC 50 value of 20 AE 1 mg/mL and 30 AE 0.8 mg/mL, respectively. More importantly, the in vivo study of the postprandial hyperglycemia activity with (20 mg/kg) of HEPdL showed a decrease in plasma glucose levels after 60 min in resemblance to the glucor (acarbose) (50 mg/kg) effect. The oral administration of HEPdL (20 mg/kg) in alloxan-induced diabetic mices for 28 days showed a more significant anti-diabetic activity than that of the drug (50 mg/kg). Moreover, cytotoxicity effects of HEPdL in IGR-39 cancer cell lines were tested by MTT assay. This extract was effective in inhibiting cancer cells growth (IGR-39) at dose 35 and 75 mg/mL. These results confirm ethnopharmacological significance of the plant and could be taken further for the development of an effective pharmaceutical drug against diabetes and cancer. 2016 Elsevier Masson SAS. All rights reserved.
Aim. The present study aimed to isolate flavonoid fraction from the aerial parts of Cissus quadrangularis and to evaluate its antioxidant and anticancer potential using in vitro assay system. Methods. Total phenolic and flavonoid contents were calculated for the drug. Flavonoid fraction was isolated using column chromatography and analysed using HPLC. In vitro, antioxidant activity of the ethanol extract and isolated flavonoid fraction was investigated by nitric oxide, DPPH and hydroxyl radical scavenging assays. Breast cancer (MCF 7) cell line was used as the in vitro cancer model for MTT assay. Result. The amount of total phenolic content and total flavonoid content in the ethanol extract showed 28.6 mg/g dry weight expressed as gallic acid equivalents, and 15.8 mg/g was expressed as quercetin equivalents, respectively. The tested extract showed good dose-dependent free radical scavenging property in all the models with the IC 50 values of 98 g/mL, 125 g/mL, and 96 g/mL for ethanol extract and 10 g/mL, 12 g/mL, and 10 g/mL for flavonoid fraction, respectively. The flavonoid fraction possess potent anticancer property against breast cancer cells (MCF7) with IC 50 value of 40 g/mL. Conclusions. It can be concluded that the aerial part of Cissus quadrangularis has potential antioxidant and anticancer activities.
Drosera burmannii Vahl: Antioxidant Potential in Dalton’s Ascites Lymphoma (DAL) Bearing Mice
The aim of the present study was to evaluate the antioxidant role of Drosera burmannii Vahl. (Droseraceae) in Dalton's Ascites Lymphoma (DAL) bearing mice. The effect of ethanol and aqueous extracts of D.burmannii (EEDB and AEDB) were administered at a 250 and 500mg/kg once a day for 14 days, 24 h after the inoculation of tumor cell line. After the treatment period, blood was collected from the animals and subsequently they were sacrificed for isolation of liver, brain, kidney and lungs for the observation of antioxidant status level. The parameters analyzed were catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), peroxidase (P), total protein (TP) and carbonyl content. Treatment with EEDB and AEDB significantly reduced the levels of MDA and increased the levels of GSH, SOD, CAT, P and TP in cancer induced animal and are similar as that of normal mice. The results suggest that the ethanol and aqueous extract of D. burmannii Vahl. possess significant antioxidant effects in DAL bearing mice.