Prevalence, Isolation and Detection of Virulent Gene in Escherichia coli from Duck (original) (raw)
Related papers
Indian journal of animal health, 2023
The present study was undertaken to assess the prevalence of Escherichia coli possessing different virulence genes in healthy ducks reared in organized farms, the backyards and their associated environments covering various agro-climatic zones of West Bengal. A total of 272 samples were collected and screened for E. coli based on cultural, morphological and biochemical properties followed by PCR for confirmation of 16S rRNA gene. A total of 342 isolates were generated. The virulence genes, viz., stx1, stx2, eaeA and ehxA were detected in 79 (23.10%), 51 (14.91%), 43 (12.57%) and 77 (22.51%) isolates, respectively. The present study revealed that healthy ducks might play a role in transmitting disease producing E. coli to other animal species including human through direct contact and/or the food chain, that warrants serious attention and strategic intervention.
A total of 120 rectal swab samples from ducks (sixty samples from Nepalgunj area of Nepal and 60 from Boyera area under Mymensingh district of Bangladesh) were collected for the isolation of Escherichia coli and their antibiogram study. After cultural and biochemical examination, a total of 40 samples from Nepalgunj and 45 samples from Boyera were found positive for E. coli. Pathogenicity study of 10 positive isolates from Nepalgunj and 12 positive isolates from Boyera were done to detect the presence of enterotoxin. All inoculated mice died showing typical lesion of extensive hemorrhage and massive edema. The isolates from two different origins showed major difference in their antibiogram study. The isolates of Nepal were highly sensitive to ciprofloxacin, co-trimoxazole, chloramphenicol and amoxicillin; moderately sensitive to nalidixic acid and; less sensitive to kanamycin and resistant to cephalexin. However, the isolates of Bangladesh were highly sensitive to ciprofloxacin, chloramphenicol and amoxicillin; moderately sensitive to nalidixic acid, cephalexin, and co-trimoxazole; less sensitive to kanamycin. This variation of antibiotic sensitivity and resistance patterns among the E. coli isolates of Nepal and Bangladesh might be due to strain variations and indiscriminate use of antibiotics in these two different countries.
E. coli isolates ( 345 from chickens and 150 from ducks) were tested for phenotypic and genotypic virulence markers. The results revealed that 285 out of 345 examined E. coli strains from chickens (82.6%) were able to bind Congo red dye and appeared as red colonies, 112out of 150 examined E. coli strains from ducks were positive for Congo red test (74.7%). 302 E. coli strains from chickens (87.5 %) were HA + , while 114 E. coli strains from ducks were HA + . 252 E. coli strains from chickens (73%) were positive for haemolytic activity, in contrast to 85 E. coli strains from ducks (56.7%). E. coli serogroups O44 and O158 isolated from chickens cases were cytotoxic to vero cells. On the other hand, serogroups (O114, O91, O111, O125, O103, O142, O26, O78, O127, O164 and O119) obtained from chickens were non-cytotoxic for vero cells. The serogroups (O44, O158, O114, O91, O111, O125, O103, O142, O26, O78, O127, O164 and O119) obtained from ducks were non cytotoxic for vero cells. The testing of E. coli strains representing 13 O groups for virulence genes isolated from chickens revealed the detection of Stx1 gene in one isolate belonging to O158 and Stx2 gene was found also in one isolate of the O group 44. The eaeA gene (intimin) was detected in E. coli isolates belonging to the O groups O44, O158, O125, O114 and O78. Iut A gene was detected in E. coli isolates belonging to the O groups O114, O44, O111, O26, O142, O78, O103 and O91. ISS gene was detected in E. coli isolates belonging to the O groups O44, O91, O111, O158, O125, O114, O142, O127, O103, O164 and O119. All of the tested 9 serogroups from ducks were negative for the Stx1 gene, and Stx2 gene, while eaeA gene was detected in E. coli isolates belonging to the O groups O158, O78 and O26; iut A gene was detected in E. coli isolates belonging to the O groups O114, O44, O91, O111, O26, O158, O78, O103 and O125 and iss gene was detected in E. coli isolates belonging to the O groups O114, O44, O91, O111, O158, O125, O103 and O26. Concerning the plasmids of E. coli isolates recovered from chickens, three bands of plasmids were detected in four O groups (O44, O158, O111, O114), two bands in O groups (O78, O103 and O125), while only one band was seen in group (O142). In duck's isolates, three bands of plasmids were detected in two O groups (O158 and O114) and two bands in one O group O44. Intratracheal inoculations of one day old chicks of different isolates of E. coli revealed that serogroups (O44, O158, O114, O111, O91 and O78) caused a mortality rate that varied between (60%-80%), while in serogroups (O103, O125, O142, O26, O127, O164 and O119), the mortality rates were much lower (30%-40%).
2012
A total of 135 fecal samples were collected to characterize the waterfowls (whistling Swan) harbored in the Hakaluki Haor of Bangladesh in the year of 2008 and 2009. Out of 135 fecal samples, 100 samples were distinguished as posi biochemical and motility test. Amongst the recovered isolates only 38% were found upbeat to enterotoxin production propensity on mice inoculation test. Finally, out of 38 % enterotoxigenic E. coli (ETEC) positive isolates no any isolates found to be positive for the aptitude of heat stable (ST) toxin yield. So, the presence of ETEC in migratory waterfowls indicating the possibilities of them to act as vector and reservoir of E. coli to spread further infection to animals and humans. This work indicates the first time ETEC characterization from the migratory birds of Bangladesh.
2007
The purpose of this study was to determine the presence of selected virulence genes in Avian Pathogenic Escherichia coli (APEC). We examined 12 APEC isolates which belonged to the most common serotypes in Iran. All 12 isolates were tested for the presence of stx1, stx2, eae, espB and hly genes by multiplex polymerase chain reaction in two protocols. In the first protocol the isolates were tested with EC and with hly primers, and in the second protocol the isolates were examined with eae, stx1, stx2 and espB primers. Seventy five percent (9) of the isolates carried only stx2 gene sequence and just one isolate had both stx1 and stx2 genes. Furthermore, 2 isolates (16.66 %) possessed eae sequence and 3 isolates carried espB (25%). The hly gene was not detected in any of the isolates. The findings of this study indicated that the Stx2 may be widespread among APEC in Iran.
Microorganisms
Avian pathogenic Escherichia coli (APEC) causes colibacillosis, which is an economically important disease in the poultry industry worldwide. The present study investigated O-serogroups, phylogenetic groups, antimicrobial resistance, and the existence of virulence-associated genes (VAGs) and antimicrobial resistance genes in 125 APEC isolates between 2018 and 2019 in Korea. The phylogenetic group B2 isolates were confirmed for human-related sequence types (STs) through multi-locus sequence typing (MLST). O-serogroups O2 (12.5%) and O78 (10.3%) and phylogenetic group B1 (36.5%) and A (34.5%) were predominant in chicken and duck isolates, respectively. Out of 14 VAGs, iucD, iroN, hlyF, and iss were found significantly more in chicken isolates than duck isolates (p < 0.05). The resistance to ampicillin, ceftiofur, ceftriaxone, and gentamicin was higher in chicken isolates than duck isolates (p < 0.05). The multidrug resistance (MDR) rates of chicken and duck isolates were 77.1% a...
Bioresearch Communications
Avian colibacillosis, caused by avian pathogenic Escherichia coli (APEC), is one of the major infectious diseases of poultry that bring about great economic loss for the Bangladesh poultry industry. The present study aimed to determine the virulence genes of avian pathogenic Escherichia coli (APEC) from cases of colibacillosis in poultry at the Noakhali district of Bangladesh. Currently, virulence-associated gene profiles of APEC isolates were investigated by polymerase chain reaction (PCR). A total of 24 (twenty-four) Escherichia coli isolates were collected and presumptively identified from 8 (eight) colibacillosis cases from 4 commercial broiler poultry farms (2 broilers per farm) in Noakhali, Bangladesh. The pathogenesis of Escherichia coli involves a wide range of different virulence genes. At this point, four virulence genes, iutA, hlyF, iroN, and iss were detected by PCR analysis. It has been observed that iutA, iss, hlyF, and iroN genes were found in 7(29.16%), 20(83.33%), 2...
Virulence Factors and Genotypic Characterization of Escherichia coli Isolated from Chickens
Suez Canal Veterinary Medicine Journal. SCVMJ, 2019
This study assesses the presence of one hundred and fourteen E. coli strains recovered from five hundred chicken examined samples including five hundred heart, five hundred livers and five hundred cloacal swabs with a percentage of 12.8 %. E. coli is one of the most common isolates in avian diseases, which causes colibacillosis, or act as a major factor in development of acute respiratory disease causing high losses especially between chickens. In addition to the conventional methods used for isolation and identification of E. coli, PCR is required as rapid, accurate and specific tool for detection of pathogenic E. coli and their virulence genes.
Serotyping and Virulence Genes Detection in Escherichia coli Isolated from Broiler Chickens
A total number of 125 chicken samples from apparently healthy broiler chickens (25 and 15), diseased broiler chickens (25 and 15) and freshly dead ones (25 and 20) were collected in winter (from December to February) and summer (from June to August), respectively from Kafr-Elsheik Governorate. In winter season, E. coli was recovered from 43 broiler chickens with an incidence of 57.3% and the incidence of E. coli in apparently healthy broiler chickens was 32%, diseased broiler chickens 64% and in freshly dead ones 76% while in summer season E. coli was recovered from 21 broiler chickens with an incidence 42% represented 26.6% in apparently healthy, 40% in diseased chickens and 55% in freshly dead one. The serogroups of E. coli that obtained by serological identification were O78, O1,O26, O2, O127, O91and O153. The results obtained by multiplex PCR reported that eaeA (intimin or E. coli attaching and effacing) gene detected in O2,O26,O1and O153, ompA (outer membrane protein) gene detected in all E. coli serogroups that isolated O2,O26,O78,O127,O1and O91 except O153. Stx1 gene detected in O2, O26, O78and O91. Stx2 gene detected in O78,O127 and O91