Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs (original) (raw)
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The effect of intradermal administration of inactive platelet-rich plasma on flap viability in rats
Acta cirurgica brasileira, 2017
To evaluate the effect of inactive form of platelet rich plasma (PRP) on the flap viability. Thirty six rats were used. Rats were divided into six groups then 9x3 cm random pattern skin flaps were elevated from dorsum of all rats. For precluding vascularization from the base, a silicone layer was placed under the flap in groups 2(only flap+silicone), 4(saline+silicone) and 6(PRP+silicone). In groups 1(only flap), 2(only flap+silicone) nothing was done except flap surgery. In groups 3(saline) and 4(saline+silicone), saline was applied intradermally , in groups 5(PRP) and 6(PRP+silicone), inactive form of PRP which obtained from different 16 rats was applied intradermally, into certain points of flaps immediately after surgery. After 7 days flap necrosis ratio was measured in all groups. Mean necrosis rate in group 5(PRP) (16.05%) was statistically significantly lower than group 1(only flap) (31,93%) and group 3(saline) (30,43%) (p<0.001). Mean necrosis rate in group 6(PRP+silicone...
Bioscience Reports
Platelet-rich plasma (PRP) is known to be rich in growth factors and cytokines, which are crucial to the healing process. This study investigate the effect of subcutaneous (S/C) infiltration of autologous PRP at the wound boundaries on wound epithelization and contraction. Five adult male mongrel dogs were used. Bilateral acute full thickness skin wounds (3 cm diameter) were created on the thorax symmetrically. Right side wounds were subcutaneously infiltrated with activated PRP at day 0 and then every week for three consecutive weeks. The left wound was left as control. Wound contraction and epithelization were clinically evaluated. Expression of collagen type I (COLI) A2, (COLIA2),histopathology and immunohistochemical (IHC) staining of COLI α1 (COLIA1) were performed on skin biopsies at first, second and third weeks. The catalase activity, malondialdehyde (MDA) concentration and matrix metalloproteinase (MMP) 9 (MMP-9) activity were assessed in wound fluid samples. All data were ...
Autologous Platelet-Rich Plasma Enhances the Healing of Large Cutaneous Wounds in Dogs
Frontiers in Veterinary Science, 2020
Platelet-rich plasma (PRP) is known to play a crucial role in skin wound healing, in both Human and Veterinary Medicine. Remarkably, until now, no studies have reported PRP treatment in subacute full-thickness skin wounds of the dog. The aim of this study was to evaluate the effects of two consecutive applications of autologous PRP, with the second application after 15 days, in 6 dogs showing large subacute skin wounds. The percentage of contraction, re-epithelialization and healing in all treated patients indicated that no complications or side effects, associated with consecutive PRP treatments, occurred in any patient and all wounds achieved complete closure and re-epithelialization. Our results suggest a positive effect of repeated autologous topical PRP treatments in large cutaneous subacute wounds of different etiology. Therefore, this PRP treatment could represent a simple, cost-effective, and valid alternative to promote healing processes in subacute large wounds cases in dogs.
Effectiveness of Pre PRP Injection and Post Elevation Flap at Extended Random Flap Rat Skin
Folia Medica Indonesiana
Background:Closure of the defect with a random skin flap is constrained by the extent of the defect area. Several studies have shown the benefits of administration of PRP (Platelet Rich Plasma) in random skin flap. The purpose of this study is to compare the effect of PRP injection given before flap elevation, after flap elevation and control on the extended random skin flap procedures in rats.Methods: This research is experimental with randomized post test only control group design (n = 27, divided into 3 treatment groups) that compare the effectiveness of PRP injection 24 hours prior to the elevation of the flap, after the elevation of the flap, and control in the extended random skin flap in rats. Random skin flap is made in the ratio 1: 5 on the rat skin. Measurement of viable area were observed on days 1, 7 and 14.Results: There were significantly increased viability of random skin flap on the group with PRP injection 24 hours prior flap elevation compared to other group of tre...
Evaluation of the effectiveness of platelet-rich plasma in surgical epidermal wounds in rabbits
Revista U.D.C.A Actualidad & Divulgación Científica, 2018
Platelet-rich plasma (PRP) is the portion of the blood plasma which contains a platelet concentration above basal levels that stimulate tissue repair by means to growth factors. The aimed of this research was to evaluate the impact of PRP on skin repair upon epidermal surgical lesions. Using a scalpel, a clean epidermal incision of 5cm length was made in the interscapular region of New Zealand rabbits (n=8), and then divided in two groups. Rabbits from the group 1 (n=4) were untreated (control group), while group 2 received PRP on the surgical wound. Autologous PRP was obtained from whole blood drawn by jugular vein puncture, collected with sodium citrate anticoagulant solution, and centrifuged at 120g x 5min. One milliliter was obtained of the phase superior to the buffy coat. Biopsies from the injured tissue were collected on days 3, 7, 14 and 21 post-surgeries, and submitted to Hematoxylin-Eosin and Masson's Trichrome staining. In conclusion, the platelets separation method allows to obtaining plasma with higher platelets concentration and reduced number of leukocytes. Additionally, the results show that therapeutic PRP administration on surgically-induced skin injury has effect on the histological features of tissue healing compared with the control group .
Effects of Autologous Platelets Rich Plasma on Full-thickness Cutaneous Wounds Healing in Goats
International Journal of Animal and Veterinary Advances, 2013
This investigation was designed to evaluate the role of Platelet-Rich Plasma (PRP) on healing of experimentally wounded skin in ten adult bucks, aged 2-3 years and weighing 25-30 kg. The animals divided randomly and equally into (control and treatment groups). Four of 3×3 cm of full-thickness square cutaneous wounds was induced on both sides of the lateral thoracic region of each animal under the effect of local anesthetic proceeding by xylazine hydrochloride as a sedative. A pair of left wounds was treated by injection with 5 mL of autonomous PRP (treatment group), 2 mm lateral to the wound edges and in the wound center. While, the right wound were injected by 5 mL of sterile saline by the same procedure (control group). Each group was divided into five subgroups (four wounds of each), for morph metrical and histopathological evaluations of wound healing process represented by percent of wound contraction, epithelialization and total healing at 3, 7, 14, 21 and 28 days post-wounding. The morphometrical appearance of the wounds which treated with PRP, showed that the contraction, re-epithelialization and healing percent were statically significant (p<0.05) in comparison with control wounds during four weeks study. Based on histopathological results, there was re-epithelialization of epidermis, with highly cellular granulation tissue, well differentiated keratinocytes of epidermis with scar formation in the dermis of the sectioned skin. We conclude that local injection of PRP leads to accelerate and improvement of wound healing in comparison to control wounds.
Diagnostic Pathology, 2015
Background: Platelet-rich plasma (PRP) contains numerous growth factors to promote wound healing and angiogenesis. The aim of this study was to gain further information about the benefits of platelet-rich-plasma for healing cutaneous acute to chronic wounds. Methods: A total of 30 New Zealand albino rabbits (n = 15/group) were randomly assigned to two experimental groups: control group, and PRP group. Bilateral resection defects measuring 3 cm were surgically created on the dorsolateral of the cutaneous in animals and the defects were randomly divided into two mentioned groups. Wound area, neovascularization, size and epithelialization were compared on days 7, 14 and 21 post-wounding. Histopathological analyses were conducted on 15 specimens from each group after sacrifice by the cellular aspects of the regeneration of the tissue. Results: Our results were indicated that the wound area of PRP was smaller than that in the non-treated group on days 7, 14 and 21. Furthermore, a significant decrease of the wound size was observed in PRP groups that were significantly greater than that in the control group. A significant increase of the mean vascular density was noted in the PRP treated groups compared to the control groups at day 14 and especially day 21. This results indicated that PRP treated group' enhanced angiogenesis at the wound beds as compared to no treatment group. Conclusion: These results could be useful for researchers in the growing fields of tissue repair and experimental wound healing. Further studies will be essential to determine the role of PRP in clinical practice.
Veterinary World, 2018
Aim: The therapeutic evaluation of the biological effect of platelet-rich plasma (PRP) used as a surgical adjunct to maintain the inflammatory process and to potentiate tissue healing, make the subject of recent research in regenerative medicine. This study was designed to evaluate the healing activity of PRP by its topical application on the skin experimentally injured in a sheep model. Materials and Methods: The study was conducted on 9 adult and clinically healthy males sheep. PRP was obtained by a protocol of double centrifugation of whole blood from each animal. After sterile skin preparation, full-thickness excisional wounds (20 mm x 20 mm) were created on the back of each animal. The animals were randomly divided into three equal groups of three sheep for each. In Group I, the wounds were treated with PRP, in Group II; wounds were treated with Asiaticoside; in Group III, wounds were treated with saline solution. The different treatments were administered topically every 3 days. Morphometric measurements of the contraction surface of the wounds and histopathological biopsies were carried out at the 3rd, 7th, 14th, 21st, and 28th days of healing. Results: The results of the morphometric data obtained revealed that it was significant differences recorded at the 7th and 14th day of healing in favor for animals of Group I. Semi-quantitative histopathological evaluation showed that PRP reduces inflammation during 3 first days post-surgical and promotes epithelialization in 3 weeks of healing. Conclusion: We concluded that topical administration of PRP obtained by double centrifugation protocol could potentially improve the skin healing process in sheep.
An Experimental Study on the Effect of Platelet-Rich Plasma Application on Skin Graft Healing
Journal of Traditional Medical Complementary Therapies
There is new information increasingly added about wound healing, which is one of the most complex physiological processes. In this study, it is aimed to evaluate the effect of plateletrich plasma on the wound healing process in pursuit of creation of skin defect on the back skin of the rat and application of graft to it. Material and Methods: The study was planned on 30 randomly selected inbred Sprague-Dawley rats. In the rats in the experimental group, 3x2 cm full-thickness skin defect was created after the back area was shaved. The skin taken from the defected area was thinned and turned into a full-thickness skin graft and sutured again to the area where it was taken. Platelet-rich plasma was applied under the graft to the rats in the 1 st group, and physiological saline was applied to the 2 nd group. The 3 rd group did not undergo any surgical procedure, and it was created as the control group. Results: Significant differences were observed in glucose, lactate, glycerol, and pyruvate values in the tissue in Group 1, compared to other groups. In the histopathological evaluation, a significant decrease was detected in the rate of inflammation and edema in the Group 1, in comparison with the other groups. Conclusion: Based on the data obtained from this study, it was taken into consideration by us that platelet-rich plasma application is a method that can be applied in addition to the classical wound care, especially in the cases with wound healing problems.
Effects of Autologous Platelet-Rich Fibrin (PRF) Therapy on Wound Healing in Dogs
2022
Background: Platelet-rich fibrin (PRF), also recognized as leukocyte-rich-PRF, is a recent platelet-based biomaterial, pointed as an innovative regenerative strategy for the treatment of wounds, from different etiologies. This study aimed to evaluate the efficacy of autologous PRF as a regenerative therapy for the treatment of skin wounds of multiple etiology, in dogs. The authors postulated that autologous PRFs could be successfully used as a biocompatible patch to increase the physiologic wound regeneration, acting as a biological antiseptic biomaterial, in spontaneous open large or chronic wounds in dogs. Eight dogs, aged between 7-months to 9-year-old, with naturally occurring cutaneous wounds were enrolled in this study. Four of these wounds were infected. The PRF treatment was initially performed two times per week, followed by single weekly treatments from the second week onwards, until exophytic granulation tissue was present. The study was finalized when complete wound clos...