Advances in NMR and MS-based Metabolomics (original) (raw)
Mass spectrometry, almost always coupled with chromatographic separation, is one of the techniques of election able to perform a comprehensive investigation of the "metabolome". MS is almost "universal" and characteristics like sensitivity and dynamic range make it an ideal tool for this task. For exactly the same reasons, however, the results of a MS-based metabolomic investigation are sensitive to any issue occurring during sample preparation, sample analysis and data preprocessing. Errors occurring in these phases will affect all the downstream statistical analysis following the well known "garbage in, garbage out" principle. The aim of this tutorial is to highlight the most critical aspect which should be taken into consideration when designing a (successful!) MS-based metabolomics assay. The discussion will touch lab practice, quality assessment and data preprocessing. The objective is to describe the strategies which can be used to control the maj...
Loading Preview
Sorry, preview is currently unavailable. You can download the paper by clicking the button above.