Separation and identification of phenolics and flavonoids from wild Pistacia palaestina extract and its antioxidant activity (original) (raw)
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Antioxidant Potential and Total Phenolic Compounds of Extracts and Fractions of Pistasia atlantica
International Journal of Pharmaceutical and Clinical Research
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2020
The pistachio industry in Tunisia generates a high amount of waste every year due to the large pistachio production. This, together with the good properties of the compounds that can be extracted from these by-products, makes it necessary a comprehensive study of their potential. Because of that, the aim of this work was to analyze the chemical characterization of male and female leaves and hull of Pistacia vera L. and their ethanol-water extracts. With that purpose, the total phenolic and flavonoid contents of the extracts were determined and their antioxidant activities were investigated using DPPH, ABTS and FRAP methods. The total phenolic content varied from 218to533mg GAE/g DE, and the total flavonoid content varied from119to 397 mg CE/g DE. In general, the antioxidant activity showed significant values. The DPPH assay and the ABTS assay were in the range of 332–704 mg TE/g DE and 427and 1394 mg TE/g DE, respectively .In the case of FRAP, male leaves extract had the best result...
Preliminary antioxidant profile of Pistacia integerrima Stewart
Pakistan journal of pharmaceutical sciences, 2014
To explore the free radical scavenging properties of crude ethanolic extract of galls, bark, leaves, roots of Pistacia integerrima and its subsequent solvent fractions viz., n-hexane, chloroform, ethyl acetate and methanol against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) stable. In vitro DPPH based free radical was employed using querceitin as standard antioxidant while methanol as negative control. Different parts of P. integerrima showed marked scavenging on DPPH in a concentration dependent manner. The ethanolic extract exhibited 60.51 88.51% scavenging effect on DPPH which differentiated upon fractionation. of the part used, leaves of the plant were the least effective while n-hexane was the least dominant fraction. However, the rest of the parts and fractions demonstrated profound scavenging potential. This in-vitro study revealed an outstanding free radical scavenging potential of various solvent fractions of different parts of whole plant P. integerrima.
Natural Product Research, 2015
The fruits of Pistacia khinjuk Stocks were collected from Ilam province, Iran. The aim of this study was to analyse antioxidant capacity and phenolic composition of different parts of Pistacia khinjuk fruit. The antioxidant capacity of extracts were measured using different assays: FRAP, DPPH and nitric oxide radical scavenging. The phenolic composition of Pistacia khinjuk fruit is reported for the first time. Among different parts of the fruit analysed in this study, hull extract contained the highest total phenolic and flavonoids contents. We observed a high correlation between different antioxidant activity and total phenolic and flavonoid contents. Therefore, antioxidant capacity can be related to total phenolic and flavonoid contents. A correlation analysis revealed that ascorbic acid, gallic acid, rutin, caffeic acid, ferulic acid and sinapic acid were the phenolic compounds mainly responsible for antioxidant power of the fruit extracts.
2015
The fruits of <i>Pistacia khinjuk</i> Stocks were collected from Ilam province, Iran. The aim of this study was to analyse antioxidant capacity and phenolic composition of different parts of <i>P. khinjuk</i> fruit. The antioxidant capacity of extracts was measured using different assays: ferric reducing ability of plasma, 2,2-diphenyl-1-picrylhydrazyl and nitric oxide radical scavenging. The phenolic composition of <i>P. khinjuk</i> fruit is reported for the first time. Amongst different parts of the fruit analysed in this study, hull extract contained the highest total phenolic and flavonoid contents. We observed a high correlation between different antioxidant activity and total phenolic and flavonoid contents. Therefore, antioxidant capacity can be related to total phenolic and flavonoid contents. A correlation analysis revealed that ascorbic acid, gallic acid, rutin, caffeic acid, ferulic acid and sinapic acid were the phenolic compounds main...
Antioxidant Activity of Pistacia vera Fruits, Leaves and Gum Extracts
Iranian journal of pharmaceutical research : IJPR, 2012
The side effects of synthetic antioxidants have been considered in different studies. Accordingly, there is an increasing interest toward the use of natural substances instead of the synthetic ones. In this study, the aqueous and ethanolic extracts of Pistacia vera leaves and fruits as well as hydroalcoholic extract of gum were tested for a possible antioxidant activity using in vitro methods. Deoxyribose assay, erythrocyte membrane lipid peroxidation and liver misrosomal non- enzymatic lipid peroxidation tests were used as an in-vitro model for determination antioxidant activity. The extract were evaluated at different concentratios: 25,100, 250, 500 and 1000 μg/mL. In all procedures, all extracts showed free radical scavenging activity. The effect of ethanolic extract of P. vera fruit at 1000 μg/mL was quite similar to positive control (DMSO 20 mM) in deoxyribose method. In two other tests, the ethanolic extracts of fruits and leaves were more effective than the aqueous extracts t...
Ecological Engineering & Environmental Technology
Pistacia lentiscus L. (PL) is a shrub belonging to the Anacardiaceae family, used in traditional medicine to treat various disorders in the Commune of Ajdir from Al Hociema province. The conducted study focused on determination of the polyphenols and flavonoids contents by spectrophotometric and in vitro evaluation of the antioxidant and anti-inflammatory capacity. Determining total polyphenols shows the aqueous extract with a higher concentration of 125.04±0.01 mg EAG/g ES, followed by the ethanolic extract of 108.16±0.02 mg EAG/g ES. Determination of flavonoids revealed that the hexanoic extract contains a maximum of flavonoids with a level of 90.60±0.01 mg EQ/g ES. The antioxidant activity of different extracts was determined through two methods: DPPH and FRAP. Aqueous and ethanolic extracts showed a high antioxidant capacity. Further, regarding anti-inflammatory activity, the ethanolic extract has good activity inhibition (92.65±0.67) followed by aqueous extract (94±0.29) at 1000 μg/mL concentration. This study found that the ethanolic extracts from P L leaves are a powerful natural antioxidant and effective anti-inflammatory agent. The results indicate the extract's effectiveness and highlight the importance of medicinal plants from the Commune of Ajdir.
Pharmaceutical biology, 2017
The widespread use of Pistacia atlantica Desf. ssp. (Anacardiaceae) in traditional medicine can be partly attributed to the content of its secondary metabolites, in particular, the phenolic compounds. The effects of harvest period, growing region and gender on the phenolic compounds, flavonoids and condensed tannins contents were studied, as well as on the antioxidant activities of P. atlantica leaves in order to provide a scientific basis for optimal collection. Leaves were collected monthly from April to October 2010 in two Algerian sites. The powdered leaves were used for preparing the ethyl acetate extract. Contents of total phenolics (TPC), flavonoids (FC) and condensed tannins (CTC) were determined spectrophotometrically. Antioxidant activity was evaluated through radical scavenging activity (RSA) of 2,2-diphenyl-1-picrylhydrazyl (250 μM) and the reducing power capacity (RPC) determination by K3Fe(CN)6 (1%). The TPC was found to vary from 79 ± 13 to 259 ± 8 mg gallic acid equi...
Antioxidant Activity of Pistacia Lentiscus Methanolic Extracts
2018
Description of the subject: The use of synthetic antioxidant molecules is currently being a health issue because of their potential toxicological risks. Now, several researches on plants as new sources of natural antioxidants are being developed. Objective: For this purpose we were interested to study the antioxidant activity of methanolic extracts of a plant from the Algerian flora: Pistacia lentiscus. Methods: Methanolic extracts were prepared from both parts of the plant (leaves and/ or fruits). The quantitative estimation of the total phenols, flavonoids and tannins content present in the extracts was carried out by colorimetric methods. The antioxidant proprieties of the extracts were performed using two methods: DPPH (2, 2-diphenyl-1-picrylhydrazil) free radical scavenging activity and the ferric reducing power (FRAP). Results: Colorimetric assays showed that the methanolic extracts of leaves and /or fruits contain high amounts of total phenols, tannins and less amounts of fla...