Burkholderia thailandensis as a microbial cell factory for the bioconversion of used cooking oil to polyhydroxyalkanoates and rhamnolipids (original) (raw)
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2019
Background and objective: Polyhydroxyalkanoates are environmentally friendly bioplastic compounds produced via the microbial route that offer an alternative to synthetic plastics due to their comparable durability and thermal stability. However, the high production cost as a result of carbon feedstock for microorganisms and the downstream recovery process narrow the usage of polyhydroxyalkanoates in various fields. Conversion of by products from the food and agricultural industries such as waste cooking oil, glycerol, palm sludge oil, oil palm trunk sap and soya waste into polyhydroxyalkanoates is an attractive approach that can minimize and/or add value to waste. Results and conclusion: Recently, there has been a lot of interest in exploring not just polyhydroxyalkanoates as valued-added products, but also PHA-producing bacteria as a nutritional food or feed source. It has been previously reported that the PHA-producing bacterium, Cupriavidus necator , can be utilized as a single c...
Microbial Cell Factories, 2019
Background In comparison to synthetically derived surfactants, biosurfactants produced from microbial culture are generally regarded by industry as being more sustainable and possess lower toxicity. One major class of biosurfactants are rhamnolipids primarily produced by Pseudomonas aeruginosa. Due to its pathogenicity rhamnolipid synthesis by this species is viewed as being commercially nonviable, as such there is a significant focus to identify alternative producers of rhamnolipids. Results To achieve this, we phenotypically screened marine bacteria for biosurfactant production resulting in the identification of rhamnolipid biosynthesis in a species belonging to the Marinobacter genus. Preliminary screening showed the strain to reduce surface tension of cell-free supernatant to 31.0 mN m−1. A full-factorial design was carried out to assess the effects of pH and sea salt concentration for optimising biosurfactant production. When cultured in optimised media Marinobacter sp. MCTG107...
The Potential of Polyhydroxyalkanoate Production from Food Wastes
2019
Background and objective: Over 1 billion tons of foods are wasted every year (not consumed by humans or animals). Most of this waste ends up in landfills. As the global population increases, mankind must look for more sustainable means of living. A recently popular idea is the use of organic wastes as carbon feedstocks for fermentation that produces value added products. Polyhydroxyalkanoates are a family of bio-based, biodegradable polymers that can be produced in large quantities using food and food processing wastes as the main feedstocks. In many cases, biocatalysts have been engineered to efficiently use these waste compounds to produce large quantities of useful intracellular polyhydroxyalkanoates. Results and conclusion: In the current study, various polyhydroxyalkanoates were produced; each with different thermal and mechanical characteristics useful for different applications. If polyhydroxyalkanoate production facilities are established next to food waste accumulation site...
Marine Drugs
Rhamnolipids (RLs) are surface-active molecules mainly produced by Pseudomonas spp. Antarctica is one of the less explored places on Earth and bioprospecting for novel RL producer strains represents a promising strategy for the discovery of novel structures. In the present study, 34 cultivable bacteria isolated from Edmonson Point Lake, Ross Sea, Antarctica were subjected to preliminary screening for the biosurfactant activity. The positive strains were identified by 16S rRNA gene sequencing and the produced RLs were characterized by liquid chromatography coupled to high resolution mass spectrometry (LC-HRESIMS) and liquid chromatography coupled with tandem spectrometry (LC-MS/MS), resulting in a new mixture of 17 different RL congeners, with six previously undescribed RLs. We explored the influence of the carbon source on the RL composition using 12 different raw materials, such as monosaccharides, polysaccharides and petroleum industry derivatives, reporting for the first time the...