Polimorfismos dos genes metilenotetrahidrofolato redutase, fator de crescimento transformador β1 e linfotoxina‐α e susceptibilidade à artrite reumatoide (original) (raw)

2016, Revista Brasileira de Reumatologia

Background: Rheumatoid arthritis is a widely prevalent autoimmune disorder with suggested genetic predisposition. Objectives: The aim of this study is to detect the pattern of genetic polymorphism of methylene tetrahydrofolate reductase (MTHFR C677 T and A1298 C), transforming growth factor-␤1 (TGF-␤1 T869 C) and lymphotoxin-␣ (LT-␣ A252G) in patients having rheumatoid arthritis and correlate these patterns to disease activity and serum levels of tumor necrosis factor-alpha (TNF-␣), B-Cell Activating Factor (BAFF), and osteopontin. Methods: A total of 194 subjects, 90 controls and 104 patients with rheumatoid arthritis were genotyped for MTHFR C677 T and A1298 C, TGF-␤1 T869 C and LT-␣ A252G polymorphisms using a methodology based on PCR-RFLP. Also serum levels of TNF-␣, osteopontin and BAFF were measured by ELISA kits. Results: The CT genotype and T allele of MTHFR C677 T and GG genotype and G allele of LT-␣ A252G are associated with the risk of RA and with higher levels of the pro-inflammatory cytokine, TNF-␣ in patients with rheumatoid arthritis. Conclusion: Our findings suggest that there is association between MTHFR C677 T and LT-␣ A252G genes polymorphisms and increased risk of RA in this sample of Egyptian population.