Ergosterol and Lanosterol from Aspergillus nidulans (original) (raw)
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Ergosterol biosynthesis pathway in Aspergillus fumigatus
Steroids, 2008
s t e r o i d s 7 3 ( 2 0 0 8 ) 339-347 a v a i l a b l e a t w w w . s c i e n c e d i r e c t . c o m j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / s t e r o i d s Ergosterol biosynthesis pathway a b s t r a c t The sterol composition of Aspergillus fumigatus for the biosynthesis of ergosterol is of interest since this pathway is the target for many antifungal drugs in clinical use. The sterol composition of this fungal species was analyzed by gas chromatography-mass spectrometry in different strains (susceptible and resistant to azole drugs). Also, sterols were analyzed in several A. fumigatus mutant strains deficient in enzymatic steps of the ergosterol biosynthesis pathway such as 14-␣ sterol demethylases (Cyp51A and Cyp51B) and C-5 sterol desaturases (Erg3A, Erg3B and Erg3C). All sterols identified from azole-resistant A. fumigatus strains were qualitatively and quantitatively similar to the susceptible strain (CM-237). However, sterol composition of mutants strains were different depending on the lacking enzyme. The analysis of the sterol composition in these mutant strains led to a better understanding of the ergosterol biosynthesis pathway in this important fungus.
Frontiers in microbiology, 2012
Ergosterol, the major sterol of fungal membranes, is essential for developmental growth and the main target of antifungals that are currently used to treat fatal fungal infections. Emergence of resistance to existing antifungals is a current problem and several secondary resistance mechanisms have been described in Aspergillus fumigatus clinical isolates. A full understanding of ergosterol biosynthetic control therefore appears to be essential for improvement of antifungal efficacy and to prevent antifungal resistance. An ergosterol biosynthesis pathway in A. fumigatus has been proposed with 14 sterol intermediates resulting in ergosterol and another secondary final compound C-24 ethyl sterol. Transcriptomic analysis of the A. fumigatus response to host-imposed stresses or antifungal agents is expanding our understanding of both sterol biosynthesis and the modes of action of antifungal drugs. Ultimately, the identification of new targets for novel drug design, or the study of combin...
Antimicrobial Agents and Chemotherapy, 2006
Desaturases Erg3A and Erg3B: Role in C-5 Sterol Aspergillus fumigatus http://aac.asm.org/content/50/2/453 Updated information and services can be found at: These include: REFERENCES http://aac.asm.org/content/50/2/453#ref-list-1 at: This article cites 44 articles, 24 of which can be accessed free CONTENT ALERTS more» articles cite this article), Receive: RSS Feeds, eTOCs, free email alerts (when new http://journals.asm.org/site/misc/reprints.xhtml Information about commercial reprint orders: http://journals.asm.org/site/subscriptions/ To subscribe to to another ASM Journal go to: on November 16, 2014 by guest http://aac.asm.org/ Downloaded from on November 16, 2014 by guest http://aac.asm.org/
World journal of microbiology & biotechnology, 2017
Finding new compounds with antifungal properties is an important task due to the side effects of common antifungal drugs and emerging antifungal resistance in fungal strains. ∆(24)-sterol methyltransferase (24-SMT) is a crucial enzyme that plays important roles in fungal ergosterol biosynthesis pathway and is not found in humans. In the present study, the effects of α-bisabolol on Aspergillus fumigatus Af239 growth and ergosterol synthesis on the base of 24-SMT enzyme activity were studied; in addition, the expression of erg6, the gene encoded 24-SMT, was considered. To our knowledge, this is the first report demonstrating that α-bisabolol inhibits A. fumigatus growth specifically via suppressing fungal 24-SMT. Since this enzyme is a specific fungal enzyme not reported to exist in mammalian cells, α-bisabolol may serve as a lead compound in the development of new antifungal drugs. Fungi were cultured in presence of serial concentrations of α-bisabolol (0.281-9 mM) for 3 days at 35 °...
FEMS Microbiology Letters, 2005
The formation of cholesterol synthesis inhibiting molecules by five different strains of the koji mold Aspergillus oryzae was studied. After growing these strains on a complex liquid medium we found in crude organic phase extracts and specific fractions there from compounds inhibiting cholesterol synthesis in human hepatic T9A4 cells in vitro at enzyme sites downstream of dihydrolanosterol. This was evidenced by using different radioactively labeled precursors, namely acetate, mevalonate, 24,25-dihydro-[24,25-3 H 2 ]-lanosterol or [3-3 H]-lathosterol.
Analysis of Sterols by Gas Chromatography–Mass Spectrometry
Springer Protocols Handbooks, 2020
Sterols are a major component of cell membranes among all biological systems including bacteria, plant, fungi, and mammals. While the essential carbon skeleton found in all sterol-like structures is the sterane (cyclopentanoperhydrophenanthrenes) ring, there are minor variations which make each structure unique. These include hydroxylations, methylations, ketone groups, double bonds, etc. These structures play specific roles in the biological membranes. Earlier, sterols could only be detected using traditional methods like thin-layer chromatography or UV-vis spectrophotometry. However, these techniques are unable to accurately differentiate between these closely related sterol structures. Therefore, it becomes essential to develop new and sensitive methods for accurate quantification of sterols. In the last few decades, research on gas chromatography-mass spectrometry (GCMS)-based sterol structure determination and quantification has been on the rise. In this chapter, we have discussed some basic background of GCMS and its application in the absolute quantification of sterols using some examples.