Impact of cyanobacterial extracts on the growth and oil content of the medicinal plant Mentha piperita L (original) (raw)
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Journal of Medicinal Plants
Background: The cyanobacteria isolated and purified from soil can increase plant growth and productivity as bioelicitors. Objective: This study aimed was to investigate the effect of cyanobacteria as a bioelicitor on the production efficiency and essential oil composition of Mentha longifolia L. Methods: Two species of heterocystous cyanobacteria, Anabaena vaginicola ISB42 and Nostoc spongiaeforme ISB65, were used as bioelicitors to optimize the growth and productivity of Mentha longifolia L. Five pots were considered for each treatment and five others for the control plants. Treatment lasted for 100 days, and the treated plants were irrigated with 200 ml of cyanobacterial suspensions (0.2 %) every 21 days intervals. The experiment was performed in a randomized complete block design in an experimental greenhouse condition. After inoculation of treated plants with cyanobacterial bioelicitors, the vegetative factors, including shoot and root length, leaf area, as well as fresh and dry weight of plants were evaluated. In addition to growth factors, analyses of essential oils in studied plants were performed. Results: The results showed significant improvement in vegetative growth indices of the treated plants. Also, some economic and medicinal metabolites such as menthol, eucalyptol and phytol were increased in the essential oil of treated plants. The results also showed a significant increase in sesquiterpene hydrocarbons and oxygenated diterpenes in plants treated with cyanobacteria compared to controls. Conclusion: So, the use of cyanobacterial bioelicitors can be suitable to increase the yield as well as the economic and medicinal value of this medicinal plant.
Industrial Crops and Products, 2015
The occurrence of mycorrhizal association with plant roots causes an increased absorption of water and improves soil nutrient uptake, mainly phosphorus (P), what also affects the plant metabolism. This work aimed to study the growth of plants of Mentha crispa L. and their essential oil (EO) content, cultivated with and without arbuscular mycorrhizal fungi (AMF) inoculation of Glomus etunicatum and Rhizophagus clarus under different levels of P. The treatments tested were 21 mg P dm −3 soil (low level) and 200 mg P dm −3 soil (high level) added to the initial soil inoculated or not with two AMF species at the beginning of the experiment in pots filled with 3.5 kg of fumigated soil for each pots. The experiment was performed in greenhouse using a completely randomized design in a 2 × 2 factorial: 2 levels of P (Low and high) and AMF inoculation (with and without) with six repetitions. The density of spores and AMF root colonization, the plant fresh matter, and the content and composition of the EO of M. crispa were determined four months after transplantation. The density of AMF spores was not significantly affected by the P addition; however, the AMF root colonization decreased significantly when P was applied to the soil. The plant inoculated with G. etunicatum presented the highest root colonization (61%) at low P in the soil. The plants inoculated with R. clarus presented the highest production of fresh matter, shoot height and number of leaves when P was applied to the soil. The plants inoculated with G. etunicatum showed the highest EO content (0.98%) at low P in the soil. However, when P was applied to the soil, the EO content reduced to 0.09%. The EO chemical composition was not drastically affected by the treatments and 10 components were found, the main ones being limonene (3-19% of total EO composition) and carvone (72-86% of total EO composition). .br (O. Alberton).
Effect of shading and organic fertilization on the development and production of essential oil of Mentha arvensis L. (Atena Editora), 2024
Japanese mint (Mentha arvensis L.) is an aromatic plant, belonging to the Lamiaceae family, also known as peppermint, Japanese mint and vick, its oil has high levels of menthol, used in the cosmetics, beverage and pharmaceutical industries, In the Amazon region, little is known about the processes used to obtain the essential oil. Therefore, the present work aimed to evaluate the development and production of Mentha arvensis L. under different levels of shading combined with organic fertilization in the municipality of Itacoatiara-AM. The experiment was carried out in the experimental area of campus 2 of ``Universidade Federal do Amazonas``-UFAM, located on the AM-010 highway. The experimental design was conducted in randomized blocks in a 3 x 4 factorial scheme (three levels of shading and four fertilizations with manure cattle: Dry mass of the shoot (MSPA); Dry mass of the root (MSRA); Number of new shoots (NBT); were subjected to analysis of variance using the F test, and the means that were found to be significant were compared using the Scott-Knott test at 5%. The use of shade with an overshoot level of 50% influenced the growth in the number of leaves, as well as. the dosage of 20 t/ha reduced biomass production in Mentha arvensis L, in relation to cultivation in full sun, while the doses of cattle manure did not interfere significantly in biomass production, nor in other variables and physiological indices.
Journal of Biotechnology and Biodiversity
This work aimed to evaluate the production of fresh and dry mass of leaves, stems and aerial parts, and the content and quality of lemon mint (Mentha x piperita var. citrata) essential oil as a result of four potassium (K) concentrations (276, 414, 552 and 690 mg.L-1) under hydroponic solutions. The experiment was carried out in the hydroponic NFT (Nutrient Film Technique) system. Leaves were separated and weighted to determine the fresh mass and part of them was used to extract oil in a Clevenger apparatus. The analysis of the oil chemical composition was performed in a gas chromatograph fitted with a mass spectrometer. The estimated concentration for the maximum fresh mass production of the leaves corresponded to 384 mg.L-1 K. The greatest K concentration proportionated an increase in essential oil content and yield per plant, but decreased linalool and linalyl acetate in the oil. Under the conditions the experiment was carried out, in order to obtain an adequate quantity of leave...
Plant Physiology and Biochemistry, 2013
Extensive research is going on throughout the world to find out new molecules from natural sources to be used as plant growth promoter. Mentha arvensis L. is the main source of menthol rich essential oil used commercially in various food, pharmaceutical and other preparations. Experiments were conducted on field grown plants for understanding the effect of calliterpenone (CA), a stereo-isomer of abbeokutone, in comparison to gibberellic acid (GA 3) on growth attributes, trichomes, essential oil biosynthesis and expression of some oil biosynthetic pathway genes. The exogenous application of CA (1 mM, 10 mM and 100 mM) was found to be better in improving plant biomass and stolon yield, leaf area, branching and leaf stem ratio than with counterpart GA 3 at the same concentrations. CA treated plants showed higher glandular trichome number, density and diameter and also correlated with enhanced oil biogenetic capacity as revealed by feeding labeled 14 C-sucrose for 72 h to excised shoots. Semi-quantitative PCR analysis of key pathway genes revealed differential up regulation under CA treatments. Transcript level of menthol dehydrogenase/menthone reductase was found highly up regulated in CA treated plants with increased content of menthone and menthol in oil. These findings demonstrate that CA positively regulated the yields by enhanced branching and higher density of trichomes resulting into higher accumulation of essential oil. The results suggest CA as a novel plant derived diterpenoid with growth promoting action and opens up new possibilities for improving the crop yields and essential oil biosynthesis in qualitative and quantitative manner.
Journal of Pure and Applied Microbiology, 2021
Mentha essential oil is one of the most utilized essential oil in the food and pharmaceutical industries. The present study reports the chemical composition and antibacterial properties of leaf essential oils of Mentha species. Further, the effect of the harvesting period on essential oil yield was also investigated. Firstly, the cultivated Mentha piperita and wild Mentha longifolia, revealed significant differences in their chemical profile. M. longifolia essential oil was characterized with endo-borneol (1.12-6.2%), caryophyllene (2.72-7.03%), isopipertenone (0.07-0.36%), germacrene D (0.98-3.22%), 3-cyclopentene-1-one,2-hydroxy-3-(3-methyl-2-butenyl)- (21.91-56.72%) and piperitone oxide (8.96-39.31%), whereas, M. piperita leaves essential oil was found rich in isomenthone (5.97-6.75%), 1-menthone (7.32-18.32%) and menthol (18.03-58.53%), etc. The essential oils of both Mentha species exhibited strong antimicrobial activity as evaluated using poisoned food technique, dry weight me...
Journal of the Science of Food and Agriculture, 2010
BACKGROUND: The aim of the present study was to appraise variation in the chemical composition, and antimicrobial and cytotoxic activities of essential oils from the leaves of four Mentha species -M. arvensis, M. piperita, M. longifolia and M. spicata -as affected by harvesting season. Disc diffusion and broth microdilution susceptibility assays were used to evaluate the antimicrobial activity of Mentha essential oils against a panel of microorganisms. The cytotoxicity of essential oils was tested on breast cancer (MCF-7) and prostate cancer (LNCaP) cell lines using the MTT assay. RESULTS: The essential oil contents of M. arvensis, M. piperita, M. longifolia and M. spicata were 17.0, 12.2, 10.8 and 12.0 g kg −1
Asian Journal of Agricultural Research
This study was carried out to evaluate the response of Mintha piperita var. citrata (Eau de Cologne mint) to foliar fertilization under Egyptian conditions. This study was carried out on Mintha piperita var. citrata (Eau de Cologne mint) at Sekem Experimental field. Fresh, healthy, insect and disease free suckers were transplanted in furrow at a depth of 4-5 cm as per the treatments. Three weeks later after transplanting, the plants were sprayed with aqueous solution of the test nutrient compounds humic acid (0. 2.5 and 5 g LG 1) and amino spot (0, 1 and 1.5 mL LG 1). The crop was harvested in mid-May (First cutting) and mid-August (Second cutting). Growth and yield characters were measure. The essential oil percentage was determined in both cuts from fresh herb. The essential oil was analyzed by GC/Mass. It can be observed that, humic acid and/or amino spot fertilizer (Algae extract) had a significant effect on growth characters during both cuts. Increasing amino spot doses increased growth characters (plant height, herb fresh and dry weight) at all doses in the two cuts. The results show that there were clear significantly positive trend in increasing growth characters by spraying of humic acid. The interaction effect was significant in both cuts, the highest values of plant height, herb fresh and dry weight (g plantG 1) were produced from the treatment sprayed with humic acid at 5 g LG 1 +amino spot at 1.5 mL LG 1 , followed by the treatment sprayed with 2.5 g LG 1 humic acid+1.5 mL LG 1 amino spot at the two cuts. During the 1st cut, humic acid or amino spot fertilizer had a significant effect on essential oil percentage and yield (mL plantG 1) while, all treatments produced significant effect on oil percentage and oil yield (mL plantG 1) except the interaction treatments, which had no significant effect on essential oil percentage during second cut. Linalool and linalyl acetate were the main constituents of essential oil of this plant. All treatments or cuttings had a pronounced effect on essential oil constituents. Based on the experimental results it is recommended to treat Mintha piperita var. citrata (Eau de Cologne mint) plants with humic acid at 5 g LG 1 +amino spot at 1.5 mL LG 1 to produce high mass production and oil yield.