The effects of aqueous ginger extract on pancreas histology and on blood glucose in normal and alloxan monohydrate-induced diabetic rats (original) (raw)
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In recent years, ginger has become a subject of interest because of its beneficial effects on human health. The purpose of the present study was to investigate the effects of daily oral administration of ginger extract for 6 weeks on plasma glucose, lipid profile and kidney functions in alloxan-induced diabetic rats to show the ameliorating and partly curative effects in alloxan induced-diabetic rats (150 mg/kg i.p.(Intrapretonial). Rats (130-150gm) were divided into 4 groups; normal control rats, diabetic control rats, diabetic rats post-treated with ginger and diabetic rats pretreated with ginger. Ginger extract was administered orally for 6 weeks to post-treated and pre-treated rats, and they were compared with the normal and diabetic groups, respectively. Plasma glucose was reduced significantly in both post-treated and pretreated groups. The post-treatment with ginger extract reduced plasma cholesterol, triglyceride and LDL-cholesterol, but during the pre-treatment with ginger extract produced insignificant change only in plasma triglyceride level. The plasma HDLcholesterol was significantly increased in post-treated and pre-treated groups. The plasma creatinine, urea and uric acid levels were significantly reduced in post-treated group; also in pre-treated group, they were reduced but urea level statistically did not change. It is concluded that the consumption of ginger produced a significant hypoglycemic effect in diabetic rats. In addition, ginger is capable of improving hyperlipidemia and the impaired kidney functions in alloxan-induced diabetic rats.
Effects of ginger juice on blood glucose in alloxan induced diabetes mellitus in rats
Journal of Dhaka Medical College, 2015
Objective: To find out the effects of ginger (Zingiber officinale) juice blood glucose in alloxan induced-diabetes mellitus in rats. Methods: This experimental study was done in the Department of Pharmacology & Therapeutics, Dhaka Medical College, Dhaka, in collaboration with the Departments of Pathology, Ibrahim Medical College, Dhaka between January and December 2009. This experimental animal study was divided into two parts, which were Experiment-1 and Experiment-2. Experiment 1 comprises of 12 rats and divided into 2 groups each group having 6 rats. Rats of group-A was non-diabetic normal control group and group-B was fed with ginger (Zingiber officinale) in a dose of 4ml/kg body weight orally through Ryles tube. Experiment-2 comprised of 12 rats divided into 2 groups each containing 6 rats labeled as group C, group D. Rats of groups C administered alloxan 150 mg/kg intraperitoneally on the 2nd day of the study. Rats of group D were administered alloxan 150mg /kg intraperitonea...
Evaluating the Anti-Diabetic Effect of Ginger Powder in Experimental Rats
Pakistan BioMedical Journal
In the current research paper, the effect of ginger powder on diabetic rats was probed. The bio-adequacy study was observed on Alloxan prompted Wistar rodents by taking ginger powder at three levels for example 1%, 3% and 5% ginger powder for a time of about a month. There were 4 gatherings (one was control and the other three getting various rates of ginger powder), each containing 5 rodents. The blood tests were gathered at multi day, fifteenth day and 28th day. Glucose and insulin levels were estimated. The information in this manner acquired was genuinely investigated to discover the degree of importance. Results showed that glucose levels essentially diminished alongside critical expansion in insulin levels. This examination configuration infers that ginger powder has constructive outcomes in bringing down glucose levels. Subsequently, use of ginger powder and ginger tea ought to be expanded in diabetic patients to diminish hyperglycemia in diabetic patients and defeat from hig...
The Histological Effect of Aqueous Ginger Extract on Kidneys and Lungs of Diabetic Rats
International Journal of Biology, 2018
Diabetes is a disorder affecting various-aged humans and which can with time cause serious problems for the patient. Medicinal plants are known for their hypoglycemic effects; and among which is ginger (Zingiber officinale) known also for its culinary uses. This study, therefore, was undertaken to evaluate the histological effect of 21-day treatment of aqueous ginger extract used at 500 mg / kg BW on female diabetic rats. Fifteen female albino rats were divided into three groups; Group I: control, Group II: non-treated diabetic, and Group III: ginger extract-treated diabetic rats. The ginger extract-treated diabetic group received the daily dose orally for three weeks. Results show that organ weight was not significantly changed. Light microscopic examination of 5µm sections of extract-treated Kidney and Lung of the diabetic rats revealed approximately normal histological structure compared with the untreated ones. The normal appearance of glomeruli and alveoli as well as the ...
The present study evaluated the effect of ginger on blood glucose and oxidative stress status in alloxan induced diabetic rabbits. Forty eight (control), group II (diabetic control) and group III, and IIV (diabetic rabbits) treated with ginger ethanolic extract as a single daily does. 250mg\kg, 500mg\kg body weight of extraction dissolve in l cc DW orally for 6weeks respectively. Treatment with ginger significantly reduced (p<0.05) the level of glucose, and MDA, while significantly increased (p<0.05) levels of the GSH,GPX when compared with the control group. The results indicate that ginger in addition to being anti-hyperglycemic is effective in reducing oxidative stress in diabetes.
Hepato-Protective and Antioxidant Effect of Ginger on Hepatic Tissue in Experimental Diabetic Rats
2015
Antioxidant defense system plays a key role in diabetic complications. The effects of ginger ethanolic extract on ruined antioxidant system in liver tissue remains unclear in diabetic rats. In this study, we investigated the impact of oral administration of ginger ethanolic extract on antioxidant parameters in experimental diabetic rats. Thirty rats were equally divided into five groups, including normal control (NC), diabetic control (DC), ginger treatment (Gt), diabetics treated with ginger (D+Gt) and diabetics treated with glibenclamide (D+Glb). The parameters like SOD, CAT, GPx, GR, GST activities and GSH levels were significantly decreased in liver of diabetic rats, which indicates impaired antioxidant status. The lipid peroxidation marker MDA levels were drastically increased in diabetic rats. However, we found that the administration of ginger to diabetic rats restored the altered antioxidant status that is almost similar to normal control group, simultaneously depleted MDA l...
Few studies have determined the effect of different ginger extracts on insulin sensitivity, very few have explored the effect of raw extract of this spice on this parameter while the effect of cooked ginger extract (the form in which the spice is commonly consumed) is yet to be ascertained. This experimental study was therefore designed to determine the effect of raw and cooked ginger extracts on insulin sensitivity in normal and high-fat diet-induced diabetic rats. Ginger rhizomes were washed, peeled, wet-milled and sieved without adding water to obtain the raw extract. The raw extract was boiled for 1 hour to obtain the cooked extract. Seventy male albino rats of weight range 120-160g were divided into seven groups (n=10) and designated thus: ND 2 C-non-diabetic control; ND 2 R-non-diabetic rats given raw ginger extract (2ml/kg body weight); ND 2 Co-non-diabetic rats given cooked (boiled) ginger extracts; D 2 C-diabetic control; D 2 R-diabetic rats given raw ginger extract; D 2 Co-diabetic rats given cooked ginger extract; and D 2 D-diabetic rats given metformin (anti-diabetic drug)-200mg/kg body weight. Diabetes was induced in the diabetic groups by feeding the animals with high-fat diet (HFD) for 12 weeks to mimic Type 2 diabetes. Ginger extracts and the drug were administered as a daily oral dose for 4 weeks after diabetes induction. Insulin sensitivity (Insulin Tolerance Test) was determined before and after diabetes induction and at the end of the 4 weeks extracts administration using standard analytical method. Mean data were analyzed using ANOVA at p ≤ 0.05. Baseline Insulin Sensitivity (IS-%/min) in all the groups ranged from 2.2 to 2.4. After 12 weeks feeding, the IS in the three groups fed normal diet ranged from 1.9 to 2.1 while in the four groups fed HFD Insulin Sensitivity ranged from 0.1 to 0.3. Four weeks administration of raw and cooked ginger extracts did not significantly alter IS in normal rats while in diabetic rats raw, cooked ginger extracts and metformin significantly increased IS (%/min) to 2.1, 1.2 and 1.7 respectively relative to control which was 0.1. Insulin Sensitivity was maintained within the normal level in normal rats that were given raw and cooked ginger extracts while the raw extract was more effective than the cooked extract in increasing insulin sensitivity to normal level in diabetic rats. Both raw and cooked ginger extracts improved insulin sensitivity in high-fat diet-induced diabetic rats but the raw extract was more effective, hence, ginger in both forms may be applicable in the management of Type 2 diabetes. Human trial is hereby recommended.
Pharmacognosy Research, 2013
Background: Diabetic mellitus and hypothyroidism lead to serum lipoproteins disorders. This study aims to investigate the potential effect of fresh ginger extracts Zingiber officinale roscoe (Family: Zingebiraceae) on serum lipid profile and on blood glucose in alloxan-induced diabetes and propylthiouracil-induced hypothyroidism in rats. Rats were divided into 11 groups: The normal G1, diabetic control rats G2, ginger 500 mg/kg treated diabetic rats G3, 10 mg/day atorvastatine-treated diabetic rats G4, [5 mg/day atorvastatine combined with 500 mg/kg ginger] treated diabetic rats G5, glibenclamid-treated diabetic rats G6, hypothyoidism control rats G7, 300 mg/kg ginger-treated hypothyroidism rats G8, 500 mg/kg ginger-treated hypothyroidism rats G9, 10 mg/day atorvastatine-treated hypothyroidism rats G10, [atorvastatine combined with 500 mg/kg ginger]treated hypothyroidism rats G11. Thirty days after treatment, samples were collected, to compare treated groups with normal and control groups, using Mann-Whitney U test P < 0.01. Results: It revealed a decrease in the levels of total cholesterol (TC), and low density lipoprotein (LDL) in the serum of rats that were treated by ginger extracts, compared with the control groups. Previous extracts were also able to cause reduction in LDL to similar levels compared to normal group and that was the same effect of atorvastatin 10 mg/day. Combined effect was clear between the act of ginger at a dose of 500 mg/kg and atorvastatin; that levels of both TC and LDL in animals which received [atorvastatin 5 mg/day combined with ginger extract] was almost equal to levels in animals that received atorvastatin 10mg/day. Clear reduce in triglyceride, and clear increase in high density liopprotein were also recorded in the ginger-treated groups. Ginger was more active in hypothyroidism rats than in diabetic rats in reducing LDL and TC. Glucose levels were substantially reduced in ginger-treated diabetic groups.
CATRINA , 2015
Ginger and alpha-lipoic acid have recently gained attention as a potent antioxidant. The purpose of the present study was to investigate the possible therapeutic effects of daily oral administration of ginger and alpha-lipoic acid on diabetic rats. 42 Male albino rats (150-180gm) were divided into 7 groups; control, olive oil (vehicle) control, alloxan induced diabetic (DM), ginger-treated (GN), alpha-lipoic acid-treated (ALA), diabetic rats treated with ginger (DM+GN) and diabetic rats treated with alpha-lipoic acid (DM+ALA). After 5 weeks of GN and ALA administration (50 and 30 mg/kg b. wt respectively), hematological, biochemical and histological parameters were investigated. Diabetic rats exhibited a significant decrease in erythrocytic parameters as well as significant increase of fasting blood sugar (FBS), glycated hemoglobin (HbA1c), total cholesterol (TCh), triglyceride (TG), low density lipoprotein (LDL), very low density lipoprotein (VLDL), and a decrease in the level of high density lipoprotein (HDL). In addition, diabetic rats showed a significant decrease in hepatic GSH and an increase in the content of malondialdehyde (MDA). The administration of GN and ALA caused a significant decrease in FBS, HbA1c, TCh, TG, and LDL-cholesterol, concomitant with a significant increase in HDL-cholesterol was recorded. Moreover, GN and ALA administration blunted the increase in MDA and stimulated the GSH production in the liver of diabetic rats. The findings of this study indicated that injection of GN and ALA corrected the erythrocytic parameters, lipid profile, blood sugar level in diabetic rats and showed antioxidative properties. Additionally, the therapeutic effect of GN and ALA was confirmed with histopathological examination of pancreas. It could be postulated that; (i) GN and ALA have protective and anti-hyperglycemic effects and (ii) overall anti-diabetic effects of ALA are better than those of GN, at least in this experimental condition.
The mechanism for the blood glucose lowering effect of different ginger extracts is yet to be clearly understood, that of raw ginger extracts has been sparsely explored while the mechanism for the cooked ginger extract, the form in which it is mostly consumed, has not been delved into. This study was therefore designed to determine the effect of raw and cooked ginger (Zingiber officinale Roscoe) extracts on glucose uptake by the muscle and adipose of normal, streptozocin-induced and high-fat diet-induced diabetic rats. Fresh ginger rhizomes were washed, peeled, milled and sieved without adding water to obtain the raw extract. A portion of this was boiled for 1 hour to give the cooked extract. Matured male albino rats (140) were divided into two major groups with 70 rats in each group. Group A was further divided into 7 subgroups (n=10) 40 of which were rendered diabetic with intraperitoneal injection of streptozocin (60mg/kg body weight) to mimic Type 1 diabetes while in Group B Type 2 diabetes was induced with a 12 week consumption of High-Fat Diet (HFD). The remaining 30 rats in each group were left to remain non-diabetic/ normal. Ginger extracts were administered as a daily oral dose for 4 weeks after diabetes induction. The animals were the sacrificed and glucose uptake by the muscle and adipose tissue was determined using standard analytical procedure. Mean data were compared using Least Significant Difference at p≤ 0.05. It was clearly evidenced that both raw and cooked ginger extracts significantly enhanced and increased glucose uptake by the muscle and adipose tissues even in the absence of insulin, hence, mimicking insulin action. Ginger in both raw and cooked forms may therefore be beneficial in the prevention and management of diabetes mellitus. Human trial is hereby recommended.