Evaluating Effect of Nitric Oxide Synthase Inhibitors on Adipocyte Formation (original) (raw)

Abstract: Background: Adipose tissue can be defined as secretory cells and contains Adipocytes. Conversion of precursor cells to mature fat cells during the formation of adipocytes is controlled by transcription factors including peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein family(C/EBPα,β and δ). Nitric Oxide is synthesized from l-arginine by one of three nitric oxide synthases (NOS). Fat mass can be reduced by dietary supplementation with l-arginine. Inhibitors of nitric oxide are classified as endogenous and synthetic.The effect of nitric oxide inhibitors on adipogenesis was investigated in cultured Murine preadipocyte cell line 7F2 derived from mouse white adipose tissue. Methods: Murine preadipocyte cell line 7F2, are maintained in DMEM culture media containing 10% FCS. Induction media contains Insulin 10 ug /ul, dexamethasome 1uM, isobutylmethylxanthine 0.5mM and indomethasone 100 uM, media was changed every 3days. Culture cells treated with panels of nitric oxide inhibitors was stained with Oil red O working solution, and Griess assay was performed on the culture media in other to quantify the nitrate/nitrite level. Results: 7f2 cells have measurable oil red O staining after 7days, amount of Nitric oxide secreted can be reduced by modest amounts of nitric oxide synthase inhibitors and Oil red O staining is reduced by nitric oxide synthase inhibitors. Conclusion: Role of NOS inhibitors in adipose cells proliferation and differentiation may provide new insights into treatment of obesity and control of adiposity. Key Word: Adiposity, Nitric Oxide, Obesity Abbreviations: BAT- brown adipose tissue, WAT- white adipose tissue, PPARγ- peroxisome proliferatoractivated receptor, C/EBPα- CCAAT/enhancer-binding protein family NO-nitric oxide, INOS- induce nitric oxide synthase, L-NMMA- N-monomethyl-arginine and N, ADMA-N-dimethyl arginine , L-NIO-N-iminoethyl-Lornithine, L-NAME- N0-nitro-L-arginine methyl ester, FCS-Fetal calf serum, NOS- nitric oxide synthase, eNOSendothelial nitric oxide synthase, nNOS- neuronal nitric oxide synthase.