Soft Cheese and Grilled Chicken as a Possible Source of Human Salmonellosis with Special Attention to Human Risk Factors (original) (raw)
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Salmonellosis is one of the most important zoonotic bacterial pathogen of food-borne infection all around the world.The present study was carried out to report the prevalence of the serotypes and genetic types of salmonella among broiler chickens,raw chickens meat and patients suffer from food poisoning signs in Toukh Egypt.Samples collected from (50) diarrheic broiler chicken,(50) raw frozen chickens meat and (30) diarrheic patients with food poisoning signs were bacteriologically and serologically processed for identification of Salmonella. Isolates were subjected to multiplex-PCR using specific Salmonella primers.The prevalence of Salmonella spp was 7(14%), 2(4%) and 3(10%) in chickens,raw chickens meat and patients respectively. TheSalmonella Isolates were serologically identified as 7(58.33%) and 5[41.66%] S.enteritidis and S.typhimurium respectively.The antigenic formula of serovar S.enteritidis has somatic antigen O: 1,9,12 and phase I (g,m) and phase II [1,7] flagellar antigen (H),While serovars S.typhimurium have the O: 1,4, (5),12and phase I and phase II.Multiplex-PCR yield similar diagnostic amplified DNA bands of molecular size marker at 250bp in tested S.enteritidis and 620bp in examined S.typhimurium,indicating the zoonotic potential of the organism and the role of chicken and chicken meat as sources in the epidemiology of the human salmonellosis.
Molecular identification of Salmonella isolates from poultry and meat productsin Irbid City, Jordan
World Journal of Microbiology & Biotechnology, 2003
The sensitivity and accuracy of molecular diagnosis of Salmonella from meat and poultry products using polymerase chain reaction (PCR) was compared with conventional microbiological methods. A total of 212 samples representing the most frequently used fresh and frozen meat and poultry products (whole, cut, ground, and processed) were collected from different locations within the city of Irbid. DNA was extracted directly from each food sample and amplified using Salmonella-specific primers. Samples were also analysed using conventional microbiological methods for the presence of Salmonella spp. Results showed that Salmonella was detected in 185 samples out of 212 (87%) by PCR technique, while 172 (81%) samples were detected Salmonella positive by conventional microbiological methods. On the other hand, 27 (12.7%) samples were negative by PCR and 40 (18.8%) samples were negative by conventional microbiological methods. PCR assay proved to be an effective method for Salmonella detection in meat and poultry products with high specificity and sensitivity and more importantly a less time-consuming procedure. Using PCR, Salmonella spp. detection could be achieved within 24–36 h compared to 3–8 days for the conventional microbiological methods.
Journal of Advanced Veterinary and Animal Research, 2017
Objective: This study was conducted to determine the prevalence of Salmonella in milk (farm bulk milk, raw market milk) and cheese (kareish, white soft cheese) samples that were collected randomly from farms, supermarkets, small vendors and shops in different districts of Mansoura city, Egypt. Materials and methods: A total of 100 farm bulk milk, raw market milk, kareish cheese and white soft cheese samples (25 of each) were screened for the prevalence of Salmonella spp. The Salmonella isolates were isolated and identified by conventional bacteriological techniques, which were further confirmed genetically by polymerase chain reaction (PCR) based on the presence of invA gene. Finally, the isolates were serotyped. Results: Salmonella could be detected in 15%(n=15/100) samples with a prevalence of 12%(n=3/25), 24%(n=6/25), 20%(n=5/25) and 4%(n=1/25) in raw market milk, raw farm bulk milk, kareish cheese and white soft cheese, respectively. The Salmonella isolates were serotyped into S. enteritidis 33.3%(n=9/27) which was the most frequent, followed by S. typhimurium 25.9%(n=7/27), S. heidelberg 14.8%(n=4/27), S. infantis 11.11%(n=3/27), S. tsevie 11.11%(n=3/27) and S. haifa 3.7%(n=1/27). Conclusion: The present study confirms the presence of Salmonella in milk and cheese samples in Mansoura, Egypt, indicating that the dairy products can act as potential sources of Salmonella infection. Thus, appropriate hygienic measures are suggestive for combating Salmonellosis in Egypt.
International Journal of Microbiology and Biotechnology
Salmonella is the most common zoonotic pathogen around the world, there is an inadequate capacity of tests to detect this pathogenic bacterium in Libya. Therefore, this study was conducted to investigate the presence Salmonella spp in various foods from different food establishments in Tripoli, Libya. A total number of 370 samples were taken from 35 confectionery premises (170 samples of cakes, 25 of tarts), 11 poultry butcheries (55 samples of chicken meat), and 2 cattle butcheries (120 samples of camel meat). The isolates of Salmonella bacteria were investigated and identified by conventional cultures and biochemical methods such as (API20E). The typical Salmonella identified isolates were subjected to the PCR to detect invA gene. The results showed that 30/370 (8.11%) Salmonella spp were identified and distributed in 10 cake samples (5.9%), 2 tart samples (8%), 16 chicken meat samples (29.1%) and 2 camel meat samples (1.7%). The invA gene was detected in 22 isolates (73.33%), all Salmonella spp isolated from cakes and cattle meat samples are invasive strains. Overall, Salmonella spp is more abundant in poultry butchers than other food establishments in Tripoli, Libya, inclusion of PCR methods to detect Salmonella spp is highly recommended.
Türkiye’de 2015-2020 yılları arasında hayvansal orijinli Salmonella serovarlarının dağılımı
Etlik Veteriner Mikrobiyoloji Dergisi
Salmonella is an important zoonotic microorganism and the most isolated among food-borne infections across the world, including Türkiye. The detection and identification of common serovars circulating in Türkiye can present very useful data in the fight against Salmonella, which threatens both animal and human health. This study aimed to determine the distribution and diversity of Salmonella serovars isolated from the samples sent to Bacteriological Diagnosis Laboratory of the Veterinary Control Central Research Institute. The serotyping results of a total of 1,047 Salmonella spp. strains isolated between 2015 and 2020 were retrospectively evaluated. After confirming that the samples isolated were Salmonella spp. according to ISO 6579, identification at the species level was carried out by serotyping with the slide agglutination test. A total of 19 serogroups and 75 Salmonella serovars were detected. The most commonly isolated Salmonella serovar was Salmonella Infantis (40.5%), foll...
This study was carried out to investigate the occurrence of Salmonella species in 690 different samples collected from Sharkia Governorate, Egypt. The samples comprised of chicken meat, organs, eggs, cloacal swabs and wooden surface swabs from pluck shop outlets and hand swabs from workers. In addition, stool swabs from patients with gastroenteritis attending the Outpatient Clinics at Zagazig and Abo-Hammad cities. Salmonella spp. were detected in 5.9%, 2.6%, 4.2%, 10.4%, 10% and 10% of chicken meat, organs, egg shell, cloacal swabs, surface swabs and hand swabs, respectively. The isolation rate from stool swabs was 0.8%. Biochemical Identification revealed that Salmonella spp. were identified in 129 out of 690 examined samples (18.7%). Meanwhile, molecular identification using invA gene revealed that only 29 Salmonella isolates were detected (4.2%). S. Typhimurium and S. Enteritidis were identified from different samples with the percentages of 1.2 and 1.01, respectively. The other identified Salmonella serovars were S. Newport (0.9%), S. Kentucky (0.7%), and S. Infantis (0.4%). Virulence associated genes including avrA, hilA and pef were identified in 100, 91.3 and 10.3% of the examined isolates. In conclusion, a proportion of chicken carcasses and giblets sold in Sharkia Governorate, Egypt was contaminated with Salmonella spp. including potentially virulent S. Typhimurium and S. Enteritidis, posing risk for human consumers.
Afyonkarahisar İlindeki Yenebilir Sakatatlarda Salmonella’nın Prevalansı
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2012
In this study, the prevalence of Salmonella sp. was examined using a total of 205 edible bovine offal samples collected from different abattoirs (n=105) and butcheries (n=100) by immunomagnetic separation (IMS) method. The isolation rate of Salmonella was found to be 8.57%, 5.71% and 5.71% for the liver, kidney and tripe samples collected from the abattoir, respectively. Of the offal samples obtained from the butcheries, the detection rate of Salmonella sp. was 16% in the liver and 4% in the kidney, tripe and brain samples. Overall, it was found that 8.29% of all the offal samples obtained from the abattoirs and butcheries were determined to be contaminated with Salmonella sp. The results of this study shows that edible offal are cross-contaminated by Salmonella sp. at the abattoirs and retail sale points until they reach to the consumer. It is recommended that adequate hygienic and sanitary measures be taken in these kind of places in order to protect public health.
Prevalence of Salmonella spp. in Packed and Unpacked Red Meat and Chicken in South of Tehran
Jundishapur Journal of Microbiology, 2014
Background: Despite of the advances in infectious diseases prevention and food technology, food-borne diseases are considered major problems in developed and developing countries. Meat plays a key role in transferring zoonotic diseases to human. Objectives: This study was conducted in south of Tehran, Iran, to investigate the prevalence rate of Salmonella spp. in packed and unpacked red meat and chicken. Materials and Methods: A total of 379 packed and unpacked samples including 189 red meat and 190 chicken samples were collected randomly. From each sample, 25 g was separated and treated with 225 mL of buffered peptone water, homogenized and incubated at 37°C for 24 hours. Samples were enriched using Rappaport-Vassiliadis broth and then streaked onto Hektoen enteric agar. Results: Totally, 86 out of 190 chicken and 38 out of 189 red meat samples were contaminated with Salmonella spp. The most isolated serotypes were Salmonella thompson (67.7%), S. heaardt (6.5%), S. enteritidis (4.8%), and S. veyle (4%), respectively. In general, the rate of chicken contamination was higher than meat, as 43.3% of packed and 46% of unpacked chicken samples were contaminated. Conclusions: These results confirmed the pervious findings, stating that proper packaging of meat products can effectively decreases the rate of microbial contaminations.
The Southeast Asian journal of tropical medicine and public health, 2012
Food borne Salmonella infection is an important cause of morbidity and mortality. A total of 200 food samples commonly sold in Lagos, Nigeria comprising raw and cooked meat as well as meat products and spoilt meat were analysed for the presence of Salmonella spp using REVEAL serology kit, culture methods employing RPVA (Rappaport Vassiliadis agar), SSA (Salmonella-Shigella agar) and BSA (brilliant sulphite agar) and PCR method for direct detection from samples using primer salm3/4 and ST11/ST15 sets. Using the REVEAL serology kit, 74% of the samples were positive for Salmonella spp, while culture methods showed only 19% to be Salmonella spp. The PCR method revealed that Salmonella spp was present in 62% and 54% of the samples using primer set salm3/4 and ST11/ST15, respectively. However, the primer set ST11/ST15 was more reliable in the identification of Salmonella spp directly from food samples. These tools should prove useful in the continuous monitoring and control strategies esp...
Veterinary World, 2014
Aim: To evaluate the hygienic quality of raw chicken meat and raw milk sold in the local markets of Patna with reference to isolation of Salmonella and antibiotic resistance pattern of Salmonella against commonly used antibiotics. Materials and Methods: A total of 370 samples comprising of 228 chicken meat and 142 market milk samples were processed for isolation and serotyping, supplemented with molecular detection of isolates targeting invA gene of Salmonella. All the isolates were tested against commonly used antibiotics (13 nos). Results: Out of 370 samples, 23.7% (54/228) chicken meat and 7.7% (11/142) milk samples were found positive for Salmonella based on biochemical reactions. The serotyping of Salmonella isolates showed an incidence of 6.1% of Salmonella typhimurium, 2.6% of S. newport, 1.7% of S. gallinarum and 0.4% each of S. enteritidis, S. infantis and S. worthington in chicken meat; and 2.1% of S. typhimurium and 1.4% of S. newport in market milk samples. Polymerase chain reaction targeting invA gene showed positive presence of Salmonella in 18.42% chicken meat and 5.6% market milk samples. Conclusion: The antibiotic susceptibility test revealed the presence of multiple drug resistant Salmonella in chicken meat and milk. The present study indicates high prevalence of Salmonella in raw chicken meat and milk due to poor hygienic practices and therefore emphasizes the need for adopting these hygienic practices.