Development of an enzymatic assay for ethanolamine in plasma (original) (raw)

Ethanolamine (Etn) contributes to the generation of phosphatidyl ethanolamine (PE), a membrane phosphatide. A study reported that urine Etn was increased in newborns with Zellweger syndrome, a congenital metabolic disease. And more, recently, a metabolomics study, using mass spectrometry, reported that the saliva Etn of pancreatic cancer patients was significantly increased compared to that of normal. However, these have not yet become clear clinically. Baba et al. reported that the reference interval of Etn in healthy volunteer’s serum is 11.84 ± 4.15 μmol/L. However, the clinical significance of Etn in adult blood was not clarified. Etn is measured conventionally by high performance liquid chromatography (HPLC) and capillary electrophoresis-mass spectrometry. These methods, however, have drawbacks including complicated preprocessing operations, and wasting of measuring time and introduction and maintenance costs. Therefore, the aim of this study was to develop a simple, rapid enzy...