Pharmacologyonline 2: 715-721 (2011) Kasote et al. 715 ATIOXIDAT AD ALPHA-AMYLASE IHIBITORY ACTIVITY OF METHAOL EXTRACT OF COLOCASIA ESCULETA CORM (original) (raw)
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ANTIOXIDANT AND ALPHA-AMYLASE INHIBITORY ACTIVITY OF METHANOL EXTRACT OF COLOCASIA ESCULENTA CORM
In the present investigation antioxidant and salivary alpha-amylase inhibitory activity of methanol extract of Colocasia esculenta corm was evaluated. The antioxidant activity of methanol extract of C .esculenta (CME) was evaluated by FRAP, DPPH, and ferric reducing power assay. Similarly, total phenol content and salivary alpha-amylase inhibitory activity of CME was evaluated by Folin-Ciocalteau and DNS method respectively. The result of total phenolic content assay reveals that CME containing 0.0137 + 1.33 mg GAE/g dw of CME. The observed resultant antioxidant activity of CME in all studied models was moderate as compared with reference standard BHT and BHA. At 1 mg/ml, CME showed salivary alphaamylase inhibitory activity with IC50 value of 0.94 mg/ml. In conclusion, from the results of present study it is confirmed that antioxidant and alpha-amylase inhibitory activity of methanol extract of C .esculenta may contribute in its earlier observed antidiabetic potential. Pharmacologyonline 2: 715-721 (2011) Kasote et al.
A Tioxida T a D Alpha-Amylase I Hibitory Activity of Metha Ol Extract of Colocasia Escule Ta Corm
2011
In the present investigation antioxidant and salivary alpha-amylase inhibitory activity of methanol extract of Colocasia esculenta corm was evaluated. The antioxidant activity of methanol extract of C .esculenta (CME) was evaluated by FRAP, DPPH, and ferric reducing power assay. Similarly, total phenol content and salivary alpha-amylase inhibitory activity of CME was evaluated by Folin-Ciocalteau and DNS method respectively. The result of total phenolic content assay reveals that CME containing 0.0137 + 1.33 mg GAE/g dw of CME. The observed resultant antioxidant activity of CME in all studied models was moderate as compared with reference standard BHT and BHA. At 1 mg/ml, CME showed salivary alphaamylase inhibitory activity with IC50 value of 0.94 mg/ml. In conclusion, from the results of present study it is confirmed that antioxidant and alpha-amylase inhibitory activity of methanol extract of C .esculenta may contribute in its earlier observed antidiabetic potential.
Journal of medicinal plant research
In the present study various extracts of ten medicinal plants, collected in Iran, were examined for α-glucosidase and α-amylase inhibition using an in vitro model. Also total phenol content and antioxidant activity of the extracts were investigated. Various extracts of the plants (Cinnamomum zeylanicum, Crataegus oxyacantha, Hibiscus sabdariffa, Morus alba, Portulaca oleracea, Rubus fruticosus, Syzygium aromaticum, Teucrium polium, Trigonella foenum-graecum, and Vaccinium arctostaphylos) were prepared using n-hexane, dichloromethane, chloroform, ethyl acetate and methanol. Methanol, dichloromethane and n-hexane extracts of S. aromaticum exerted high in vitro inhibitory potential against α-glucosidase and α-amylase with ranging from 0.3 to 1.1 and 36.2 to 41.9 µg/ml, respectively. The mentioned extracts possessed the highest total phenolic contents (139.8, 119.6 and 136.1 mg GAE/g of extract). The antioxidant activities of the extracts, measured in terms of IC50 values were 2.2, 3.9 ...
Antioxidant and alpha amylase inhibitory activity of Nepalese medicinal plants from Gorkha district
Journal of Pharmacognosy and Phytotherapy
The aim of this study is to evaluate the antioxidant activity, α-amylase inhibition activity, estimation of total phenolic and flavonoid content and the toxicity in ten medicinal plants Woodfordia fructicosa, Tectaria coadunate, Prunus cerasoides, Abrus precatorius, Eclipta prostrate, Poranopsis paniculata, Chenopodium album, Oroxylum indicum, Curcuma caesia, and Butea monosperma collected from Gorkha District of Nepal. Methanolic extracts of all the plants showed the presence of different phytoconstituents such as alkaloids, polyphenols, flavonoids, terpenoids, saponins, glycosides, and quinones. The highest radical scavenging was observed in methanol extract of P. cerasoides with IC 50 = 7.54±0.223 µg/ml. The potency of the radical scavenging effect of P. cerasoides was about six times greater than standard ascorbic acid (39.85±0.025 µg/ml) taken. P. cerasoides showed high phenol content (805.48±0.024 mg GAE/g extract) whereas total flavonoid content varied from O. indicum (16.96±0.015 mg QE/g extract) to W. fructicosa (722.76±0.108 mg QE/g extract). The methanol extract of E. prostrate was found to be toxic against brine shrimp as shown in the LC 50 value of 6.3 µg/ml. T. coadunate and A. precatorius showed effective results with an IC 50 value of 80.89 and 70.29 µg/ml respectively in α-amylase inhibition test. This study provides some scientific support for traditional uses of plants for diabetes management and other ailments. Since extracts of W. fructicosa and P. cerasoides are rich sources of bioactive chemical constituents, further in-vitro and in-vivo bioactivity of these extracts need to be studied for their exact mechanism of action.
Journal of Medicinal Plants Research, 2013
In the present study various extracts of ten medicinal plants, collected in Iran, were examined for αglucosidase and α-amylase inhibition using an in vitro model. Also total phenol content and antioxidant activity of the extracts were investigated. Various extracts of the plants (Cinnamomum zeylanicum, Crataegus oxyacantha, Hibiscus sabdariffa, Morus alba, Portulaca oleracea, Rubus fruticosus, Syzygium aromaticum, Teucrium polium, Trigonella foenum-graecum, and Vaccinium arctostaphylos) were prepared using n-hexane, dichloromethane, chloroform, ethyl acetate and methanol. Methanol, dichloromethane and n-hexane extracts of S. aromaticum exerted high in vitro inhibitory potential against α-glucosidase and α-amylase with IC 50 ranging from 0.3 to 1.1 and 36.2 to 41.9 µg/ml, respectively. The mentioned extracts possessed the highest total phenolic contents (139.8, 119.6 and 136.1 mg GAE/g of extract). The antioxidant activities of the extracts, measured in terms of IC 50 values were 2.2, 3.9 and 0.7 µg/ml, respectively. C. zeylanicum was another traditionally used medicinal plant, which its extracts exhibited high hypoglycaemic effect by inhibition of α-glucosidase and α-amylase (IC 50 ranged from 0.5 to 8.7 and 37.1 to 52.5 µg/ml, respectively). The obtained results support the traditionally use of a number of the analyzed species.
Pharmaceutical Chemistry Journal
Diabetes mellitus is the metabolic disease leading to elevation of blood glucose, which may be due to the lack of enough insulin or responsible tissue become insulin resistance. The main aim of the present study was to evaluate in-vitro glucose diffusion and alpha-amylase inhibition as well as to determine free radical scavenging effects of selected plant sample. The five different plants i.e., Amomum subulatum Roxb., Choerospondias axillaris (Roxb.) B.L. Burtt, Nephrolepsis cordifolia (L). C. Presl, Musa sp. And Myrica esculenta Buch.-Ham. ex D. Don were collected from different area of Kaski district, Nepal. Myrica esculenta stem bark showed potent DPPH free radical scavenging effect in both solvent which showed IC50 value of 5.920µg/ml. Amomum subulatum, Choerospondias axillaris, Nephrolepsis cordifolia fruit and leaves and Musa species leaves in water have IC50 value of 0.81 mg/ml, 0.69mg/ml, 0.49mg/ml, 0.77mg/ml and 0.61mg/ml showing better alpha amylase inhibition. Glucose diffusion inhibition study revealed that Amomum subulatum seed, Choerospondias axillaries fruit and Musa species. Leaves showed highest GDRI% in water at concentration of 20mg/ml and 40 mg/ml. Thus, Myrica esculenta stem bark, Choerospondias axillaris fruit and Musa species leaves were found potent antioxidant and alpha amylase inhibitors.
Colocasia esculenta (L) Schott of the family Araceae is an herbaceous perennial plant cultivated as annuals. Local name of C. esculenta is Kochu in Bangladesh and Taro in India. C. esculenta (L) is an ancient crop grown throughout the humid tropics and is widely used throughout the world; Africa, Asia, the West Indies, and South America. Its edible corms and leaves are traditionally used for hepatic ailments. Juice obtained from stems. Schott is used to stop bleeding from cuts and Wounds. The young leaves and roots are rich in Vitamin C as well as starch. It contains calcium, phosphorours, thiamine, riboflavin, niacin, oxalic acid, calcium oxalate, sapotoxin and flavones, apigenin and luteolin. The biological properties as well as chemical constituents of plant C. esculenta were widely used in folk medicine. In traditional medicine, C. esculenta is used as Anti microbial, Antihepatotoxic, Anti-cancer, Anti-Lipidperoxidative, Antibacterial and Antifungal, Antidiabetic, Antimelanogenic, Anthelmintic. In present study, C. esculenta shows potent pharmacological activity such as Antimicrobial, Antihepatotoxic, Anti-cancer, Anti-Lipidperoxidative, antibacterial and Antifungal, Antidiabetic, Anti-melanogenic, Anthelmintic. Due to present of several potential chemical constituents it may use in several disease conditions for the future treatment.
Journal of Pharmaceutical Research International
Management of blood glucose level is the hallmark in the treatment of diabetes. Much work has not been done on the management of diabetes using the stem tuber extract of Colocasia esculenta. The objective of this study was to evaluate the antihyperglycemic and hematological parameter on Colocasia esculenta aqueous stem extract in alloxan induced diabetic rats. Sixty (60) male rats were used in the study. Seven days of acclimatization, the rats were divided randomly into six groups of five in each group. Group 1: Served as normal control, Group 2: Diabetic control group (negative control), Group 3: Diabetic group and “Glucinorm-M80” (positive control), Group 4: Diabetic group and extract at 200 mg/kg bodyweight, Group 5: Diabetic group and extract at 400 mg/kg, Group 6: Diabetic group and extract at 600 mg/kg. Diabetes was induced in albino rats by intraperitoneal injection of alloxan at a single dose of 120 mg/kg body weight in groups 2 to 6 after starving them for 24 hrs. The anima...
Iranian Journal of Toxicology, 2020
Background: In Nigeria, the leaves of Manihot esculenta (Crantz) are eaten as vegetables. The leaves are rich in phytochemicals, valuable and natural sources of antioxidants, and are highly useful for human health and disease prevention. This study was aimed to evaluate the in vitro inhibitory effects of two extracts derived from the plant leaves on α-glucosidase and α-amylase. The total flavonoid and phenolic contents of the extracts were also assessed. Methods: The leaves of M. esculenta were processed and extracted with ethanol and acetone. The extracts were evaluated for their α-glucosidase and α-amylase inhibitory activities. Also, their total flavonoid and phenolic contents were determined, using standard in vitro assays. Results: The ethanol extract exhibited a higher α-glucosidase and α-amylase inhibitory activity than the acetone extract, which positively correlated with their total flavonoid and phenolic contents. In addition, the ethanol extract strongly inhibited the α-g...
Clinical Phytoscience
Background: The purpose of the current study is to identify the phytochemicals as well as to determine the level of antioxidant, cytotoxic, antimicrobial and in vivo analgesic, anti-inflammatory and antidiarrheal activity of leaf methanolic extract of Colocasia affinis Schott (LMCA). Methods: To appraise the antioxidant activities the extensively used total phenol, flavonoid, total antioxidant capacity, ferric reducing antioxidant power, cupric reducing antioxidant capacity and DPPH scavenging assay have been used. Cytotoxic potential was determined by brine shrimp lethality test whereas antimicrobial activity was assessed by disc diffusion method. Acetic acid induced writhing and formalin induced paw licking methods were employed to evaluate analgesic activity. To gauge anti-inflammatory activity, xylene-induced ear edema and carrageenan induced paw edema methods were used. Castor oil induced diarrhea and magnesium sulfate induced enteropooling methods were used to figure out antidiarrheal activity evaluation. Results: Phytochemical screening affirmed the presence of alkaloids, flavonoids and tannins in the extract. LMCA contains marked amount of total phenol (3.89 ± 2.36 mg/g), total flavonoid (905.50 ± 2.12 mg/g) and total antioxidant (245.83 ± 2.36 mg/g). Remarkable cupric reducing power and ferric reducing power capability were observed. In DPPH radical scavenging assay, the extract showed moderate scavenging potential (IC 50 395.97 μg/ml), whereas IC 50 value of standard ascorbic acid was 32.75 μg /ml. The extract showed a significant result in cytotoxicity test. In disk diffusion antimicrobial assay LMCA manifested broad spectrum of activity. In acetic acid induced writhing test, the highest dose (1000 mg/kg) showed 57.69% inhibition of abdominal constrictions compared to Diclofenac Sodium (74.62%). In case of formalin induced analgesic activity test the extract exhibited preeminent effect (LMCA 1000 mg/kg showed 58.64% inhibition). The extract inhibited 86.36% xylene induced ear edema (at dose 1000 mg/kg) where Aspirin inhibited 88.81%. In carrageenan induced test LMCA 1000 mg/kg manifested eloquent inhibition of inflammation at 6th hour (50.59%). The highest dose 1000 mg/kg of LMCA showed significant (80.95%) reduction in diarrhea and noticeable reduction of intestinal fluid secretion (24.29%). Conclusion: To recapitulate, it is suggested that the leaf of Colocasia affinis Schott might be a potential source of useful bioactive molecules.