A comprehensive proteomics study on edible bird’s nest using new monoclonal antibody approach and application in quality control (original) (raw)
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Characterization of edible bird’s nest by peptide fingerprinting with principal component analysis
Food Quality and Safety, 2017
OBJECTIVES: Proteins are the major component and play a key role in nutritious and therapeutic functions of edible bird's nest (EBN); however, limited studies have been conducted on the protein due to difficulties in extraction, isolation as well as identification. This study aimed to provide comprehensive information for the quality evaluation of EBN peptides, which would be a valuable reference for further study on EBN proteins. METHODS: Here, we developed a quality control method using high performance liquid chromatography (HPLC) peptide fingerprints deriving from EBN being digested with simulated gastric fluid. The characteristic peptide peaks were collected and identified by LC-MS/MS. RESULTS: The characteristic peptide peaks, corresponding to the protein fragments of acidic mammalian chitinase-like, lysyl oxidase, and Mucin-5AC-like, were identified and quantified. Interestingly, the principal component analysis indicated that the fingerprints were able to discriminate colour of EBN (white/red) and production sites (cave/house) of White EBN on the same weight basis. As proposed by the model developed in this study, Muc-5AC-like and AMCaselike proteins were the markers with the highest discriminative power. CONCLUSIONS: The overall findings suggest that HPLC peptide fingerprints were able to clearly demonstrate peptide profile differences between genuine and adulterated EBN samples; and classify EBN samples by its color and production site. In addition, the protein identification results suggested that Muc-5AC-like protein was the major protein in EBN.
Characterization and Extraction Influence Protein Profiling of Edible Bird’s Nest
Foods
The edible bird nest (EBN) from Aerodramus fuciphagus has been consumed as a Chinese traditional food for health and medicinal purposes due to its elevated nutritional value. The present study focused on the influence of characterization and extraction methods on protein profiling, which could be a guideline for grading the EBN. The proposed extraction method is similar to the common food preparation methods of consumers and thus can accurately establish the bioactive protein available upon human consumption. The characterization includes physicochemical analysis (physical, morphology, elemental composition, and microbial content) and chemical analysis (crude protein and amino acid). The morphology of half-cup EBN was found to be uniformly shaped and rich in calcium as compared to rough surface of stripe-shaped EBN, and there was no significant microbial growth in both types of EBN. The crude protein and amino acid content in half-cup EBN were significantly higher than stripe-shaped...
In Silico Analysis of Edible Bird’s Nest Proteins as Potential Precursors for Bioactive Peptides
Malaysian Applied Biology
The present study aimed to perform an in silico evaluation of edible bird’s nest protein as potential precursors of bioactive peptides, as well as to determine whether such peptides can be released by selected proteolytic enzymes. Six edible bird’s nest (EBN) protein sequences from a previous study were chosen as potential precursors to produce bioactive peptides via in silico method using the BIOPEP database. AMCase protein sequences gave the highest number of bioactivities (16 to 18) and nucleobindin-2 protein gave the lowest number of bioactivities (9) among the other protein sequences. It was found that the most potential bioactive peptides from EBN proteins are angiotensin-converting enzyme (ACE) inhibitors and dipeptidyl peptidase-IV (DPPIV) inhibitors. Furthermore, in silico proteolysis using six selected enzymes was employed to release both dominant bioactivities in EBN proteins, which were ACE and DPP-IV inhibitors. This study shows that a combination of enzymes, chymotryps...
The objective of this research is to see the comparison of protein characterization from three kinds of Indonesia edible bird nest as they have not been researched before. This research would give us overall picture on protein characteristics of the three Indonesian edible bird's nests, so it could show their activeness compared to the white edible bird nest. This research was a laboratory research with three treatments and five parameters. Treatments in this research was conducted on three kinds of edible bird nest, the white edible bird nest (Collocaliafuciphaga), the black edible bird nest (Collocalia maxima) and the swallow edible bird nest (Collocalialinchi). Parameters measured in this research were protein content, glycoprotein content, SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel) CBB (Coomassie brilliant blue) staining, SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel) glycoprotein staining and LC-MS/MS(Liquid Chromatography-mass Spectroscopy/mass spectroscopy) analysis. It was an explorative research. The method used was trial method with randomized complete design (protein and glycoprotein content) and descriptive method. The analyses results on protein and glycoprotein content from the three Indonesian edible bird nests were different and presumably due to their different kinds of feed. Protein characterization using SDS-PAGE CBB staining showed protein band high intensity on various molecular weights. They are 140.8; 64.8; 21.2 kDa for the white edible bird nest, 27.1; 21.2; 15.2 kDa for the black edible bird nest, and 140.8; 116; 36.2 kDa for the swallow edible bird nest. Glycoprotein characterization using SDS-PAGE glycoprotein staining showed different results; 140.8 and 64.8 kDa for the white edible bird nest; 140.8 and 116.0 kDa for the swallow edible bird nest; 28.4 and 105.3 kDa for the black edible bird nest. Analysis results using LC-MS/MS showed the presence of glycoprotein in the white edible bird nest but none was found in the black and the swallow edible bird nest.
Sains Malaysiana
Edible bird's nest (EBN) is a salivary secretion of swiftlets which consist of protein and carbohydrate rich glycoproteins. This natural ingredient is very valuable, nutritional and medically valuable. The EBN industry have grown rapidly and benefited the Malaysian economy, hence, it is viewed seriously and it is actively supported by the government. This review discusses the progress and development of EBN industry as well as the R&D activities and endeavours especially that which involves deriving peptides with biological activities from EBN and its by-product sources. Many studies have documented the therapeutic properties of EBN such as antiaging, antiviral, antioxidant, and antihypertensive. Studies have also been conducted to produce glycoprotein hydrolysates from EBN through enzymatic hydrolysis, and findings showed that these bioactive peptides increase solubility as well as antioxidant and antihypertensive activities. Enzymatic hydrolysis breaks long protein chains at s...
Searching for Active Ingredients in Edible Bird’s Nest
Journal of Complementary Medicine & Alternative Healthcare, 2018
Glycoprotein-A Longmystery on EBN Protein comprised the largest part of EBN, over half of the nest made of protein. However, the proteomics study was hindered by several limitations. The heavily glycosylated chains of peptide polymer in EBN were very difficult to be hydrolyzed, and thus the rooms for enzymatic reactions were always limited. The first report of protein on EBN, published in 1975 by a French group, indicated that the protein of EBN was actually glycoprotein [14].
Protein Profile of Edible Bird’s Nest Origin Kalimantan AndJava Islands Indonesia
2018
Edible bird’s nest (EBN) was a health food supplement produced from saliva of swiftlet especially Collocalia fuciphaga. The main composition of EBN is protein and a key factor in nutritional value and therapy. Each EBN type has a unique protein profile on SDS-PAGE electrophoresis gel. Therefore, this study aimed to analyze the protein profile of EBN from Kalimantan and Java Island would be a reference for further study on EBN biological activity. This research were used EBN from swiftlet house in Kalimantan and Java Island. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to measure the molecular weight of the protein and analyzed using PhyElp 1,4 device. Cluster analysis was used to categorize EBN samples based on similarity of protein characteristics among samples. A total of 9 samples of EBN from Kalimantan and Java Island showed different protein bands in the range of 18 to 552 kDa. The protein bands always found were 55 to 59 and 107 to 127 kDa. Edi...
Proteomics as a promising biomarker in food authentication, quality and safety: A review
Food Science & Nutrition, 2022
Since 1994, the term "proteomics" was first used and, to date, it has been used in wide applications in analytical chemistry, clinical food microbiology, biotechnology, and food technology. Proteomics is the analysis of proteins at a large scale in a particular biological system at a particular time (Boersema et al., 2015; Pandey & Mann, 2000). It is a very valuable tool in food analysis when applied to different analytical techniques (Marzano et al., 2020). Globally, food trends are drastically changing in production and consumption of food and consumers are paying huge attention to what they are eating. Therefore, knowledge of foodomics is gaining huge popularity in this segment. Foodomics is defined as studies of application of advanced omics approaches to food (Cifuentes, 2017). Foodomics includes epigenetics, transcriptomics, metabolomics, proteomics, peptidomics, and/or genomics to investigate food traceability, food