Brief Definitive Report THE SAME HUMAN ALLOREACTIVE T CELL CLONE CAN HELP BOTH B LYMPHOCYTES AND SPECIFIC CYTOTOXIC PRECURSORS (original) (raw)

2013

Abstract

It is well known that the generation of specific cytolytic activity during Mixed Lymphocyte Reaction (MLR) requires cooperation between cytotoxic T cell precursors (CTLp) and T helper cells (1-2). In humans these two cells can be distinguished and separated using the monoclonal antibody 5•9, which reacts with an antigenic determinant present on the helper-inducer containing subset and absent from CTLp (3). On the other hand it has been demonstrated that during MLR, help is also generated for the activation of B cells (4-6). In the present experiments we have used cloned alloreactive T cells to establish whether a single T helper cell can help both B lymphocytes and specific cytotoxic precursors. Materials and Methods Cell Suspensions. Peripheral blood mononuclear cells (PBM) were isolated by Ficoll-Hypaque centrifugation from HLA-A,-B,-C,-DR typed donors. PBM were used as stimulator cells after irradiation (3,000 R from a ~STCs source) or were rosetted with

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