Types of Human Papillomavirus (HPV) in Patients with ASCUS Pap Smear (original) (raw)
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Jinekoloji - obstetrik ve neonatoloji tıp dergisi, 2022
Aim: Cervical cancer screening guidelines gradually recommend human papillomavirus (HPV) DNA testing since the sensitivity of cytology is relatively low. This study aimed to evaluate correlation between HPV, cervical smear cytology and colposcopy directed biopsy results. Material and Methods: Patients who underwent colposcopy directed biopsy in Ordu University Training and Teaching Hospital between January 2018 and December 2021 were retrospectively reviewed. Patients with any high-risk HPV positivity who had cervical smear cytology and colposcopy directed biopsy results were included to this study. Results of HPV subtypes, cervical smear and histologic biopsy were recorded. Results: A total of 734 patients were included to this study. The mean age of the patients was 41.9 ± 7.36 years. Hundred and sixty-five (22.5%) patients had HPV 16 alone, 35 (4.8%) had HPV 18 alone, 354 (48.2%) had other high-risk HPV subtypes alone and 180 (24.5%) had more than one high-risk HPV subtypes. Two hundred and ninety-eight (40.6%) had HPV 16 or HPV 18 either alone or in combination with other subtypes. Majority of the patients (55.3%) had normal cervical smear results followed by ASC-US (27%). Colposcopy directed biopsy results were as follows: 452 (61.6%) normal, 199 (27.1%) CIN 1, 36 (4.9%) CIN 2, 42 (5.7%) CIN 3 and 5 (0.7%) squamous cell cancer. 66.1% of the patients with HPV 16 and/or HPV 18 had normal cervical cytology results, but they had significantly more ≥CIN 2 lesions compared to other high-risk HPV subtypes (19.8% vs 5.5%, p<0.001). There wasn't a statistical difference between having one or more than one HPV subtype in terms of severity of the lesions (p=0.474). The sensitivity and specificity of cervical smear cytology test were 55.2% and 69.2%, respectively. Conclusion: Although patients with HPV 16 and/or HPV 18 were more likely to have normal cervical cytology results, almost 20% of them had CIN 2 + lesions. Sensitivity of cervical cytology remains low and HPV DNA test with genotyping is more reliable as a screening tool.
Annals of Diagnostic Pathology, 2009
The aim of the present study was to determine the usefulness of human papillomavirus (HPV) testing for predicting cervical intraepithelial neoplasia (CIN) 1 and 2 to 3 on cervical biopsies in women who had atypical squamous cells of undetermined significance (ASCUS) and low-grade squamous intraepithelial lesion (LSIL) on Papanicolaou tests. In this prospective cohort, 167 women with abnormal cytologic examination (ASCUS and LSIL) were evaluated by colposcopy-directed biopsy and endocervical curettage. Colposcopy was performed on all study participants to obtain cervical tissue for histologic examination for detection of underlying CIN in patients with an initial cytologic test result of ASCUS and LSIL. A sample for HPV DNA detection by polymerase chain reaction was obtained. The HPV type 16 was positive in 35.4% of the 167 women with abnormal cytologic examination result in our gynecologic outpatient&amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s clinic. Histologic diagnosis of CIN 1 was found in 45 of 135 women with ASCUS and in 17 of 32 women with LSIL. According to the cytologic findings, the frequency of CIN grade 2 or 3 in patients classified as ASCUS and LSIL was 12.5% (17/135) and 18.7% (6/32), respectively. Of the ASCUS smears, 9.6% were positive for HPV type 16. The sensitivity of the HPV type 16 using polymerase chain reaction technique threshold in detecting CIN 1 and CIN 2 to 3 was 57% and 46% in ASCUS-LSIL cytologic examination, respectively. The positive predictive value of HPV type 16 ranged from 60% in patients with CIN 1 and 42% in CIN 2 to 3 in ASCUS-LSIL. By contrast, negative predictive value was 58% in patients with CIN 1 and 80% in CIN 2 to 3. The low positive predictive value of HPV testing with ASCUS smears suggests that HPV positivity could be not used for predicting the presence of CIN 2 to 3.
2008
Human papillomavirus (HPV) is a common sexually transmitted virus and is causally associated with cervical cancerous and pre-cancerous lesions. 1 HPV types infecting the genital tract are broadly divided into high-and low-risk types on the basis of their association with cervical cancer. The low-risk types are causally associated with genital warts. In an analysis of pooled data from 11 case-control studies, Munoz et al. 2 compared HPV types in cancer cases with control women and showed that 15 types, HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 73 and 82, were classified as high-risk types and a further three types, HPV 26, 53 and 66, were probable high-risk types. The Digene Hybrid Capture 2 HPV test (HC2) (Digene, Gaithersburg, MD, USA) is the only commercially available assay system for HPV detection and the only test approved by the US Food and Drug Administration (FDA). The test detects 13 high-risk HPV types (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68) and 5 low-risk types.
HPV DNA and Pap smear test results in cases with and without cervical pathology
Journal of the Turkish German Gynecological Association, 2011
The aim of the study was to determine the HPV prevalance and its relation to Pap smear, colposcopy and colposcopy directed biopsy in our region of Eskisehir, Turkey. Material and Methods: A total of 615 women who applied to the outpatient clinic between December 2009 and December 2010 constituted our study population. All patients underwent pelvic examination and Pap smear sampling. Patients who had pathological cervical appearance or Pap smear results of ASCUS, AGUS, LSIL or HSIL were referred to colposcopy. Cervical samples for HPV DNA were taken from the patients before Pap smear sampling during the routine examination or before the colposcopic evaluation. Results: Twenty six of 615 patients (4%) were HPV positive. Of these 26 patients, 12 were positive for HPV type 16, 3 for type 18, 3 for type 51, 2 for type 6, 1 for type 52, 1 for type 33, 1 for type 16 and type 31, 1 for type 6 and 52, 1 for type 56 and 90, 1 for type 39 and 66. In 4 patients with cervical cancer, and in 3 of 4 CIN III cases both HPV DNA and Pap smear were positive. In the Pap smear examination of 615 patients, cytology revealed 35 ASCUS (5.6%) 4 AGUS (0.6%), 2 CIN I (0.3%) results who were negative for HPV DNA. These patients with abnormal cytology (n=41) underwent colposcopy directed biopsy, there were 3 CIN I and 1 CIN III and all the other cervical biopsy results of these patients were benign (inflammation, chronic cervicitis). Conclusion: HPV positivity in our hospital setting is low which is compatible with other studies in Turkey. In positive HPV cases there is a good correlation between HPV type and positive cervical biopsy results.
International Journal of Gynecological Cancer, 2007
To compare the prevalence of cervical human papillomavirus (HPV) infection in Turkish women with normal Papanicolaou (Pap) smear and cervical intraepithelial neoplasia (CIN). In between March 2002 and November 2005, the study was designed as case–control study. Cytologic abnormalities in Pap smears were classified according to the Bethesda System (2001). Identification of the presence of HPV was carried out by the Hybrid Capture II test for all patients. To compare the groups, Chi-square test was used. A total of 1353 reproductive aged women were screened. Of them, 1344 (99.3%) had normal or class I Pap smear. Remaining nine cases (0.7%) had CIN at several degrees (five CIN I, three CIN II, and one carcinoma in situ). While all these nine cases with cervical pathologies had HPV, only 20 cases from the other group (1.5%) had HPV (χ2 466.1; P= 0.0001). This is the first study of the evaluation of the association between HPV and preinvasive cervical lesions in Turkish population. In sp...
The role of pap smear, HPV types and punch biopsy in evaluation of cervical pathologies
2019
Aim of study: Aim of our study is to determine the relation between high risk HPV(16,18,31,33) types, Pap smear and punch biopsy results. Material and methods: We used for our study patients which referred with abnormal cervical lesions and pathological cytology findings, from 2010-2017 years in Derince Training and Research Hospital. The data of the patients who underwent colposcopy were examined retrospectively. 151 patients were included in the study. Results: As a result of study-high risk lesions (CIN2-3) were observed in 18,18% (n=8/44) of NORMAL,10% (n=2/20) of ASCUS,27,27% (n=6/22) of LSIL,50% (n=5/10) of HSIL smear result reported patients. The result was reported as cervical squamous cell carcinoma in 1 patient with HSIL and 1 patient with LSIL. As a result, high–risk lesions (CIN2 and CIN3) were found 30,18% (n=16/53) in pathologic evaluation in HPV type 16 and 18 positive patients. In one patient with HPV18 was detected cervical squamous cell carcinoma. In our study was ...
PREVALENCE OF HUMAN PAPILLOMA VIRUS IN CERVICAL CELLS AMONG PATIENTS WITH ABNORMAL PAP SMEARS
Human papillomavirus (HPV) is the most common viral infection of the reproductive tract. Cervical cancer is by far the most common HPV-related disease. Nearly more cases of cervical cancer can be attributable to HPV infection.This study designed foe molecular detections and genotyping of human papilloma virus (HPV) by designing methods - Endpoint real time PCR for HPV detections and genotyping of HPV 16 and 18. The study includes 90 Pap smear samples of women suffering from abnormal Pap smears and 10 healthy women as a control , were collected from the Baghdad hospital ( Medical City), Oncology hospital and Al- Amal hospital (in Baghdad), during the period from April, 2015 to April, 2016. The age of women was ranged from 16 to 60 years. The molecular detection method (Designed PCR) in our study was showed that the 35% of women have HPV infections .
Cervical Carcinoma in Shatby University Hospital and its Relation to Human Papilloma Virus
Evidence Based Women's Health Journal
Objective: To detect human papilloma virus infections and its different genotypes in cervical cancer cases in Shatby obstetrics and gynecology university hospital of Alexandria medical school. Patients and Methods: An observational analytical prospective cross-sectional study was managed on 70 cervical cancer patients collected from gyne-oncology unit in Shatby obstetrics and gynecology university hospital of Alexandria medical school. Collection of the sample from all cases of the study for human papilloma virus testing and genotyping was done. Result: The study was conducted on 70 cervical cancer cases, considering human papilloma virus testing of the cases, the following results were found, 21 cases out of 70 cases (30%) were negative while 49 cases (70%) were positive. Collectively, 45 cases (64.3%) of the study cervical cancer cases were infected by high-risk HPV types (16,18,31), 4 cases (5,7%) by low-risk HPV types (6,11) and 21 cases (30%) were not infected. Conclusion: The prevalence of high risk human papilloma virus positive cervical cancer cases in al Shatby Alexandria medical school university hospital was 64.3% and 55.4% of all the present study cervical cancer cases were infected by HPV types 16 and 18, the study assured the relationship between cervical cancer and high risk HPV especially types 16 and 18 and denoting the importance of HPV vaccination during adolescence and HPV testing as a screening test to detect early preinvasive cervical lesion.
Journal of Evolution of medical and Dental Sciences, 2015
The causal role of human papilloma virus in all cancers of uterine cervix has been confirmed. HPV infection of uterine cervix is associated with development of a spectrum of benign and malignant epithelial lesions. Morphological changes in the epithelial cells associated with HPV have been reported in up to 91% of women with invasive and intraepithelial neoplasia. With over 120 specific types described, type 16 and 18 are linked mainly to cervical neoplasia. Human papilloma virus although necessary but is not a sufficient cause. There are cofactors necessary for progression from cervical HIV infection to cancer. Long term use of hormonal contraceptives, high parity, socio-economic status, tobacco smoking, and young age at coitus has been identified as established cofactors. PAP smear has become a routine method of cervical cancer screening and its clinical utilization has certain limitations like false negative rate (Elovainio et al, 1999 reported 20%). Colposcopy (introduced in 192...