Interleukin 1 alpha and beta production by cells isolated from membranes around aseptically loose total joint replacements (original) (raw)
Related papers
Evaluation of cytokines during the loosening of prosthesis in knee and hip replacements
Indian Journal of Pathology and Oncology, 2021
Total hip and total knee joint arthoplasty has become a successful orthopaedic intervention for relieving joint pain and functions. However, over the time problems may occur due to implant wear and loosening as well as due to chronic inflammation induced by increased level of bone resorbing cytokine(s) level. This study was undertaken to evaluate the level of cytokines in patients with painful THA/TKA without any sign of lysis around the implant. These patients were further planned for revision arthoplasty. We did not observe any such significant change in hematological, biochemical parameter and signs of lysis among the groups. However, a storm of bone resorbing cytokine level was observed in patients with revision arthoplasty at baseline. Furthermore, this increased level of cytokines remained sustained for early time point of revision arthoplasty. Although, with time, the level of these cytokines decreased but was still observed to be on the higher side when compared with the acc...
Journal of materials science. Materials in medicine
Aseptic loosening of prosthetic joints represents a major cause for revision surgery. Wear particles represent the initial stimulus in the production of a multi-cellular inflammatory membrane at the bone-implant interface. The mechanisms by which this membrane is established and its influence on bone resorption are central to understanding aseptic loosening. T cells have been identified as a component of interface tissue a feature confirmed in this study. Of the 15 cases studied, 12 cases (12/15) stained positive for CD3. T cell infiltration was present throughout the sections with some perivascular clustering. Multiplex PCR (MPCR) testing of eight of the 15 cases for Th1/Th2 cytokines did not show a predominance of either "type" of T cell response. Interleukin (IL)-2 mRNA expression was the most common feature (7/8) while IL-4 (6/8), IL-13 (6/8) and IFN-gamma mRNA expression (6/8) was also prevalent. IL-5 (4/8) and IL-10 (4/8) mRNA expression was less and IL-12 (3/8) mRNA...
Journal of materials science. Materials in medicine, 2002
The involvement of T cells in the progression of inflammation in response to wear debris at the interface of aseptically loosened joints is currently undefined. This cell type has repeatedly been demonstrated to be a common component of the cellular membrane, the interface, which forms between the bone and implant of total joint replacements (TJRs) [1, 2]. Three further insights into the role of this cell type in the interface were investigated here. Immunostaining demonstrated CD4 expression in 80% of the 15 cases tested while CD8 expression was present in 60% of the cases. Polymerase chain reaction (PCR) detected IFN-gamma mRNA expression in 75% of eight cases tested; in contrast IL-10 mRNA was only demonstrated in 50% of these same cases. Proteins extracted from another eight cases of revision tissue were analyzed using Western blotting for IL-17, fractalkine (Fkn) and CD40. IL-17 and Fkn were a consistent feature of all cases tested (8/8), while CD40 was undetectable in one case...
Journal of Materials Science: Materials in Medicine, 1998
The T lymphocytes are cells involved in immunologically mediated hypersensitivity reactions. They are found in the cellular infiltrate present in the interface membrane of aseptically loosened joint prostheses. Activation of these cells has been demonstrated but not the important cytokine, interleukin 2 (IL2) required for such activation. This study describes the localization of IL15 and its mRNA in macrophages in interface membranes associated with activated T lymphocytes which are proliferating (as is evident from HLA-DR and Ki67 expression, respectively). The findings provide further evidence for immune mediated processes in aseptic loosening of orthopaedic implants because IL15 has similar activities to IL2 in T lymphocyte activation.
Bone-resorbing cytokines in serum of patients with aseptic loosening of hip prostheses
The Journal of Bone and Joint Surgery, 1998
O ur aim was to determine if the serum levels of bone-resorbing cytokines (IL-1, TNF-␣, IL-6, GM-CSF) are altered in patients with aseptic loosening of a total hip prosthesis, and if such levels are influenced by the type of implant. We determined cytokine levels in sera from 35 patients before revision for failed total hip arthroplasty and compared them with those in 25 healthy donors. We also assessed the soluble receptor of interleukin-2 (sIL-2r) in serum as an indication of a specific immune reaction against the implant. Our findings showed that the sIL-2r and TNF-␣ serum level did not change. The IL-6 level was not significantly altered, but was higher in patients with TiAlV prostheses than in those with a CrCoMo implant and in patients with cemented prostheses. The IL-1 level was found to be higher in those with a TiAlV cemented prosthesis than in the control group (p = 0.0001) and other groups of patients (p = 0.003 v uncemented TiAlV, p = 0.01 v cemented CrCoMo, p = 0.001 v uncemented CrCoMo). The GM-CSF level significantly increased in patients compared with healthy subjects (p = 0.008), and it was higher in those with cemented than with uncemented implants (p = 0.01). Only patients with cementless CrCoMo prostheses had levels of GM-CSF similar to those of the control group. The highest GM-CSF concentrations were observed in patients treated with non-steroidal anti-inflammatory drugs (NSAIDs) in the last months before revision (p = 0.04). In addition, when massive osteolysis was observed, the level of GM-CSF tended to decrease to that of the control group.
Inflammation time-axis in aseptic loosening of total knee arthroplasty: A preliminary study
PLOS ONE, 2019
Objective Aseptic loosening (AL) is the most frequent long-term reason for revision of total knee arthroplasty (TKA) affecting about 15-20% patients within 20 years after the surgery. Although there is a solid body of evidence about the crucial role of inflammation in the AL pathogenesis, scared information on inflammation signature and its time-axis in tissues around TKA exists. Design The inflammation protein signatures in pseudosynovial tissues collected at revision surgery from patients with AL (AL, n = 12) and those with no clinical/radiographic signs of AL (non-AL, n = 9) were investigated by Proximity Extension Assay (PEA)-Immunoassay and immunohistochemistry. Results AL tissues had elevated levels of TNF-family members sTNFR2, TNFSF14, sFasL, sBAFF, cytokines/chemokines IL8, CCL2, IL1RA/IL36, sIL6R, and growth factors sAREG, CSF1, comparing to non-AL. High interindividual variability in protein levels was evident particularly in non-AL. Levels of sTNFR2, sBAFF, IL8, sIL6R, and MPO discriminated between AL and non-AL and were associated with the time from index surgery, suggesting the cumulative character of inflammatory osteolytic response to prosthetic byproducts. The source of elevated inflammatory molecules was macrophages and multinucleated osteoclast-like cells in AL and histiocytes and osteoclast-like cells in non-AL tissues, respectively. All proteins were present in higher levels in osteoclast-like cells than in macrophages.
The Journal of bone and joint surgery. British volume, 2003
We aimed to assess whether the immunological abnormalities which have been observed in patients with loose total hip replacements (THRs) are present in patients with a well-fixed prosthesis. We examined blood samples from 39 healthy donors, 22 patients before THR and 41 with well-fixed THRs of different types (15 metal-on-metal, 13 metal-on-polyethylene, 13 ceramic-on-ceramic). Before THR, the patients showed a decrease in leukocytes and myeloid cells in comparison with healthy donors, and a prevalence of type-1 T lymphocytes, which was confirmed by the increase in ratio of interferon-gamma to interleukin 4. Moreover, patients with metal-on-metal or metal-on-polyethylene implants showed a significant decrease in the number of T lymphocytes and a significant increase in the serum level of chromium and cobalt, although no significant correlation was observed with the immunological changes. In the ceramic-on-ceramic group, leukocytes and lymphocyte subsets were not significantly change...