Cell adhesiveness is related to tumorigenicity in malignant lymphoid cells (original) (raw)
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Cell Adhesiveness Is Related in Malignant Lymphoid Cells to Tumorigenicity
1984
Mouse lymphoma cells ($49) that grow in suspension culture were selected for increased tumorigenicity through continuous passages in syngeneic BALB/c mice. Developing tumors were classified as high grade malignant lymphoma, small noncleaved type. Variants were selected from these tumorigenic cells that were able to grow as a monolayer attached to their substrate, resembling, in this respect, fibroblastoid cells. Whereas the tumorigenic suspension-growing parental cells were able to induce progressive tumors with an inoculum as low as 100 cells per mouse, the adherent cells were unable to develop as tumors even at an inoculum of 1 x 108 cells per mouse. In addition, mice inoculated once with live adherent cells were immunized against 1 x 107 suspension-growing cells. Involvement of an immune response in the rejection of tumorigenic 49cellswassuggestedby(a)adoptivetransferexperimentsinwhichspleencellsfromimmunizedmiceprotectednaivemiceand(b)theappearanceofantibodiesintheseraofimmunizedsyngeneicmicethatspecificallyrecognizedbothadherentandsuspension−growing49 cells was suggested by (a) adoptive transfer experiments in which spleen cells from immunized mice protected naive mice and (b) the appearance of antibodies in the sera of immunized syngeneic mice that specifically recognized both adherent and suspension-growing 49cellswassuggestedby(a)adoptivetransferexperimentsinwhichspleencellsfromimmunizedmiceprotectednaivemiceand(b)theappearanceofantibodiesintheseraofimmunizedsyngeneicmicethatspecificallyrecognizedbothadherentandsuspension−growing49 cells and detected differences in [35S]methioninelabeled antigens from these cells. Antibodies raised in rabbits against adherent cells recognized three proteins of 34,000, 61,000, and 72,000 apparent molecular weight in radiolabeled adherent cell extracts that are either absent or present in small amounts in extracts of suspension-growing tumorigenic $49 cells. These findings, taken together with our previous report (
Adherent cells in tumor immunity
Cellular Immunology, 1977
The present studies explored the role of adherent cells in tumor immunity. Lymph node cells from mice bearing large tumors appeared to be maximally stimulated ifi viva and incapable of further stimulation by cells of the same tumor in vitro. Removal of the adherent cell population resulted in a marked decrease in the spontaneous background activity of the remaining nonadherent cells and allowed these cells to undergo stimulation when cultured in the presence of mitomycin-blocked tumor cells. The role of the adherent cell in the maintenance of a state of continuous stimulation was further elucidated by experiments in which lymph node cell populations were reconstituted from the adherent and nonadherent subpopulations. It was also shown that adherent lymphoid cells from tumor-bearing mice, but not from normal mice, were capable of stimulating tumor-immune lymphocytes in a manner similar to intact mitomycinblocked tumor cells.
Carlsberg Research Communications, 1984
Two types of "spontaneous" malignant alteration in vitro of ST/a mouse lung fibroblasts (ST-L) have been observed. In contrast to cells which retained their fibroblastic appearance (RST-L cells), cells showing morphological signs of transformation (R+ST-L cells) developed strong isoimmunizing properties. Both types ofcells expressed MuLV antigens which were found to be responsible for serum as well as cell-mediated immune reactions in vitro. The higher concentration ofgp70 in R+cells as compared to R-cells and possibly also the morphological differences in surface structure between the two cell types may account for the differences in immunogenicity. Preimmunization with R+ST-L cells protected ST/a mice against secondary challenge with two ascites tumors (STABAL leukemia and Ehrlich). RST-L cells did not have a similar protective effect. However, the two ascites tumors only showed weak or no cross-reactions in vitro with sera and lymphoid cells from ST/a mice sensitized to R+ST-L cells, and the in vitro reaction between the latter cells and sera or lymphoid cells from mice immunized against the two ascites tumors was moderate. This discrepancy between'in vivo and in vitro observations is discussed.
International Journal of Cancer, 1987
Lymphocytes immune to a highly immunogenic ("xenogenized") variant of a murine lymphoma-which were shown to exert anti-xenogenized tumor activity in a previously described model of tumor immunotherapy-were tested in the present study for possible suppressive effects on the growth of an i.c. graft of the original lymphoma. Remarkable tumorinhibitory effects followed the i.v. infusion of splenic lymphocytes sensitized or restimulated in vitro, or derived from animals immunized in vivo with the xenogenized tumor cells. The pattern of inhibition of the parental cells was apparently similar to that previously described for the xenogenized variant, but preliminary evidence suggests that the underlying mechanisms may be different.
Lymphoid cell subpopulations infiltrating into autologous rat tumors undergoing rejection
Cancer research, 1984
Lymphoid cell subpopulations infiltrating into autografts of methylcholanthrene-induced sarcomas in rats immunized with autologous tumor cells were identified in terms of immunohistochemical and cytofluorographic techniques using various monoclonal antibodies raised against different classes of rat lymphohemopoietic cells. These antibodies included in this study directed to rat T-cell antigens corresponding to mouse Lyt-1 (RLyt-1) and Lyt-2,3 antigens (RLyt-2) and to W3/25 antigen expressed on a particular subset of rat T-cells with helper function, as well as to rat granulocyte-macrophage-specific antigen (RGM-1). Histological studies demonstrated that the autografts of highly antigenic tumors introduced to the primary hosts were completely rejected following massive immigration of lymphoid cells into the tumor sites, which was not observed in progressively growing, minimally antigenic tumors. These lymphoid cells found within regressing highly antigenic tumor autografts were ident...
Adhesion of lymphoid cells to fibroblasts in tissue culture
Cellular Immunology, 1989
In this study we have examined the cellular and molecular specificity of lymphocyte interaction with fibroblasts. Using mitogen-activated T-cells, we found that attachment to fibroblasts was highly sensitive to protease treatment, and to an antibody raised against the purified lymphocyte plasma membrane, but it was not mediated by the MEL-14 surface antigen or phosphoman-nosy1 receptors. Lymphocyte interaction with fibroblasts was also unaffected by monoclonal antibodies against the LFA-1, Mac-1, and Class II MHC antigen complexes. In contrast, adhesion of both T-and B-lymphocytes was strongly inhibited by fucoidan, a polymer of sulphated fucose, whereas fucose, mannan, and mannose 6-phosphate had no effect. Both Band T-lymphoid cell lines were able to recognise and adhere to fibroblasts, although the marked differences between the attachment of the different types of cell did not appear to be related to their immunological function. The attachment of most of the cell lines was prevented by the presence of fucoidan, whereas the inhibition of binding of each of the lymphoid lines in the presence of the anti-T-lymphocyte plasma membrane antibody varied widely. These findings suggest that lymphocyte attachment to libroblasts involves multiple cell surface receptors, and that these are expressed at different levels on specific T-and B-cells. 0 1989 Academic PPXS, Inc.
International Journal of Cancer, 1979
The correlation between tumorigenicity and cell-surface adhesiveness was investigated in eight mouse fibroblastoid cell lines. Four of the lines (Mc 11-Mc 15) were derived from mouse MC-induced fibrosarcomas, two were derivatives of L cells (A9, A9HT), and two (clone 3 and clone 7 H7F4) were obtained by fusion of A9HT cells with normal diploid mouse lymphocytes. Quantitative comparison of cellular adhesive properties by the Latex particle adherence assay indicated that higher malignancy of some lines (Mc 11, 13, 14, A9HT cl 7 H7F4) was regularly associated with lower cell-surface adhesiveness and, conversely, lower malignancy of the other lines (Mc 15, A9, cl 3) with high cell-surface adhesiveness. The differences in adhesiveness of cell lines of high and low malignancy were determined by cell subsets not attaching any Latex particles (24.6–61.9% of cells in highly and 4.6–12.0% in slightly tumorigenic lines) and cell subsets attaching more than 25 Latex particles per cell (14.6–17.2% In highly and 46.1–71.4% in less tumorigenic lines).
International Journal of Cancer, 1970
A line of lymphoreticular cells (AL-I-G) that can adhere to glass surfaces has been derived from suspension cultures of Burkitt lymphoma cells (AL-1). By light microscopy, the predominant cell type in the AL-I-G cultures has features of a neoplastic histiocyte and ultrastructurally these cells have more elaborate development of cytoplasmic organelles, such as elements of the Golgi complex and mitochondria, than the predominant cell types in the suspension cultures of AL-1. Cytogenetically, the percentage of polyploid cells in the AL-I-G cultures increased progressively from 39 % in 1967 to 100% in 1968 and 1969, while the percentage of polyploid cells in the suspension cultures remained between 12 and 24 % during the same period. No herpesvirus or any other type of viralparticle was found in the AL-1-G cultures on repeated electron microscopic examination.