Influence of Insoluble Dietary Fibre on Expression of Pro-Inflammatory Marker Genes in Caecum, Ileal Morphology, Performance, and Foot Pad Dermatitis in Broiler (original) (raw)
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Kafkas Universitesi Veteriner Fakultesi Dergisi
The effect of dietary lignocellulose on broilers performance, intestinal microbiota and morphology, pH of digesta and litter humidity after 28 and 42 days of the experiment was evaluated. A total of 384 Cobb500 chickens (initial weight: 41.88±1.56 g) were divided into 4 groups with 24 replications and fed with control diet (C), a control diet with added 0.4% of lignocellulose (T1), a diet with added 0.6% of lignocellulose at the expense of soybean meal and maize (T2), and a diet supplemented with 0.6% of lignocellulose at the expense of soybean meal (T3). T2 treatment significantly influenced body weight, weight gain (WG), feed intake (FI) and feed conversion ratio (FCR). T2 and T3 treatment increased average LAB and Bifidobacterium spp. count, and decreased the number of Escherichia coli in the ileum and cecum, while differences in cecal Clostridium perfringens count among 0.4% and 0.6% treatments were not observed. Feeding the lignocellulose diet did not affect the relative weights of empty proventriculus, gizzard or intestines, but led to a decrease in pHs. T3 treatment caused an increase of the villi heights and significantly lower moisture content in the litter. Even though the addition of lignocellulose into broilers diet positively influenced performances, changes in intestinal microbiota and villi heights, based on the results of the present study, supplementation with 0.6% lignocellulose is recommended.
Journal of Animal Physiology and Animal Nutrition, 2015
Two experiments were conducted to determine the influence of dietary fibres on small intestine histomorphology and lipid metabolism in broilers from 1 to 21 day of age. In experiment 1, diets containing insoluble [cellulose (CEL); 2% and 4%] or soluble [carboxymethyl cellulose (CMC); 2% and 4%] fibre were fed to broilers from day 1 to 21 post-hatch and ileal tissue was collected at day 21 of age for histological evaluation. In experiment 2, broilers diet was supplemented with 0%, 1% or 2% insoluble fibre (Arbocel) during day 7 to 21 post-hatch and plasma and liver lipid metabolism were evaluated at day 21. In experiment 1, inclusion of CMC reduced body weight gain (BWG) and feed intake (FI) and increased feed conversion ratio (FCR) compared with others. Intestinal histomorphology was unaffected by CEL, but CMC led to an increase in crypt depth (CD) and serosa thickness and a decrease in villus height (VH), villus width (VW), VH:CD ratio and villus surface area (VSA), rather than control and CEL groups. Treatment did not affect goblet cell type. Moreover, the CMC-fed birds had greater total goblet cell count (GCC) as compared with others. In experiment 2, fibre inclusion was associated with increases in BWG from 7 to 14 day of age and an improvement in FCR, whereas FI was not influenced by treatments. Inclusion of fibre in the diet decreased the weight of the abdominal fat and cholesterol concentrations of liver and plasma. No significant effects on fatty acid composition of liver lipid were observed by fibre supplementation. These findings suggest dietary fibre affects performance, intestinal histomorphology and lipid metabolism in young chicks, which may directly affect poultry feeding strategies.
Journal of Food Agriculture and Environment
This study was performed to evaluate the effect of feeding multi-enzyme complex (Rovabio T-Flex TM) (ROV) alone or combined with a growth promoting antibiotic enramycin (ENRA) in a standard corn-soy ration for female broiler chicken from 0 to 30 days of age. ROV is a commercial enzyme that contains xylanase and β-glucanase. A total of 45 female chicks were randomly distributed in a randomized complete block design among 15 cages with 5 replicates/treatment. Birds were divided into 3 groups: T1 = control diet (CONT); T2 = T1 + 0.25 g/kg enzyme (ROV); T3 = T2 + 0.01% enramycin (ROV+ENRA). Cumulative feed intake (FI) and feed conversion ratio (FCR) were affected by treatment (P < 0.05; P < 0.001, respectively). However, cumulative body weight gain (BWG) was not influenced by treatment. Birds which had received CONT and ROV consumed more feed and had higher FCR as compared to ROV+ENRA. Treatment had no effect on dressing percentage, breast muscle yield or leg quarter yield (P > 0.05). As to the analysis of intestinal mucosa morphometrics as a function of treatment, duodenal, jejunal and ileal villi height were influenced significantly by treatment. Birds which had received ROV had longer villi in the duodenum and ileum than did CONT. Jejunal villi were longer in birds which had received ROV+ENRA. On the other hand, the improvements in performance of broilers fed ROV+ENRA could be ascribed to the decreased bacterial count. ROV supplementation had a positive effect on physical alterations in the structure of the small intestine but it was without effect on BWG and FCR.
British Poultry Science, 2018
1. An experiment was conducted to evaluate the effects of technical feed ingredients between 14 and 28 d of age on performance and health status of broilers (d 14-35) fed diets with a high inclusion rate of rapeseed meal as a nutritional challenge. It was hypothesized that the feed ingredients would improve health status related parameters. 2. A total of 1008 one-day-old male Ross 308 chicks were distributed over 36 floor pens and allocated to one of six iso-caloric (AME N 13 MJ/kg) growing diets (d 15-28): a control and five test diets supplemented with quercetin (400 mg/kg), oat hulls (50 g/kg), β-glucan (100 mg/kg), lysozyme (40 mg/kg) or fish oil ω-3 fatty acids (40 g/kg), with six replicate pens per treatment. 3. Dietary inclusion of oat hulls and lysozyme resulted in a reduction in broiler performance during the first week after providing the experimental diets. 4. No effect of interventions on the microbiota diversity in the jejunum and ileum was observed. Ileal microbiota composition of birds fed oat hulls differed from the other groups, as shown by a higher abundance of the genus Enterococcus, mainly at the expense of the genus Lactobacillus. 5. In the jejunum, villus height and crypt depth of lysozyme-fed birds at d 28 were decreased compared to the control group. Higher total surface area of villi occupied by goblet cells and total villi surface area in jejunum (d 21 and 28) were observed in chickens fed oat hulls compared to other groups. 6. Genes related to the growth-factor-activity pathway were more highly expressed in birds fed β-glucan compared to the control group, while the genes related to anion-transmembrane-transporter-activity pathway in the quercetin-and oat hull-fed birds were less expressed. The genes differently expressed between dietary interventions did not seem to be directly involved in immune related processes. 7. It was concluded that the tested nutritional interventions in the current experiment only marginally effected health status related parameters.
Spanish Journal of Agricultural Research, 2013
The effects of three plant extracts, i.e. lemon peel extract (LPE), orange peel extract (OPE) and Curcuma xanthorrhiza essential oil (CXEO), on the performance and gut health parameters of broilers exposed to high temperature was investigated. A total of 336 unsexed Ross 308 broilers were distributed to seven dietary treatments, a control diet and six diets containing 200 or 400 mg kg-1 feed of one of the three products between d 25-38 (12 chicks per pen, four replicates). To induce chronic heat stress, the temperature was increased to 34°C with 50% relative humidity for 5 h daily starting from d 28 until d 38. At d 38, four animals per pen were sampled for morphological characteristics (duodenum, jejunum and ileum) and microbial counts (ileo-cecal contents). Plant extracts did not affect the bird performance. The bursa weight of the control birds was lower (p < 0.05) comparing to those fed 400 mg kg-1 OPE and 200 and 400 mg kg-1 CXEO diets. Feeding 400 mg kg-1 of LPE decreased the duodenal villus:crypt ratio compared to control and 200 mg kg-1 OPE fed birds. Plant extracts did not have effect on ileal histo-morphology. Feeding with 400 mg kg-1 of LPE and CXEO caused a decrease in coliform counts in ileum and feeding of 400 mg kg-1 CXEO diet decreased coliform counts in caecum compared to control birds (p < 0.05). These results elucidate that CXEO, OPE and LPE might modify some microbial and intestinal traits, but without beneficial effect on performance of broilers under heat stress.
Journal of Animal and Feed Sciences
A fi ve-week-long experiment was performed on 960 one-day-old Cobb 500 cockerels divided randomly into ten experimental groups of 12 cages (replicates) of 8 birds each. The objective of the experiment was to estimate the effect of non-starch polysaccharides (NSP) derived from various cereals and addition of microbial enzyme preparations on growth performance, ileal viscosity, liver weight and concentration of short-chain fatty acids in broiler chicken caeca. Barley and oats, hulless barley, naked oats, and rye constituted the majority of diets. Diets were non-supplemented or supplemented with a commercial enzyme preparation. Enzyme preparations signifi cantly improved feed conversion ratio and body weight gains (P<0.05) only in groups fed diets based on naked oats and rye. In all groups, enzyme supplementation decreased intestinal viscosity and increased the concentration of acetate and total short-chain fatty acids in caecal digesta with the exception of the group fed the diet with naked oats. The results indicate that not total dietary fi bre content, but the type of dietary NSP, as well as microbial enzyme supplementation, may infl uence the intestinal viscosity and performance of broiler chickens.
Animals
The objective of this study was to examine the effects of diets supplemented with corn silk meal (CSM) and non-starch polysaccharide (NSP) enzyme on growth performance, blood biochemistry, immunological response, and growth-related gene expression in broiler chickens. A total of 270 broiler chickens were divided into six experimental groups: (1) basal diets (BD) as control; (2) BD supplemented with 0.5 g/kg feed NSP enzyme; the other four groups are CSM diets as following; (3) and (4) fed diet contain 40 and 80 kg/ton of CSM; (5) and (6) fed diet contain 40 and 80 kg/ton CSM and supplemented with 0.5 g/kg NSP enzyme. Body weight gain (BWG), feed conversion ratio (FCR), protein retention and fiber digestibility were synergistically improved (p < 0.05) when fed CSM supplemented with NSP enzyme. Moreover, a synergistic decrease (p < 0.05) in the serum glucose and total cholesterol were found. Immune organ weights and Newcastle disease virus titers were increased with CSM diets. I...