Ratlarda Hepatotoksisite ve Nefrotoksisite Oluşturan Siklosporin A’ya karşı Propolisin İyileştirici Etkileri (original) (raw)
Related papers
Effect of Croatian propolis on diabetic nephropathy and liver toxicity in mice
BMC Complementary and Alternative Medicine, 2012
Background: In the present study, we examined the antioxidant effect of water soluble derivative of propolis (WSDP) and ethanolic (EEP) extract of propolis on renal and liver function in alloxan-induced diabetic mice. In addition, we examined whether different extract of propolis could prevent diabetic nephropathy and liver toxicity by inhibiting lipid peroxidation in vivo. Methods: Diabetes was induced in Swiss albino mice with a single intravenous injection of alloxan (75 mg kg-1). Two days after alloxan injection, propolis preparations (50 mg kg-1 per day) were given intraperitoneally for 7 days in diabetic mice. Survival analysis and body weights as well as hematological and biochemical parameters were measured. The renal and liver oxidative stress marker malonaldehyde levels and histopathological changes were monitored in the liver and kidney of treated and control mice. Results: Administration of propolis to diabetic mice resulted in a significant increase of body weight, haematological and immunological parameters of blood as well as 100% survival of diabetic mice. Alloxan-injected mice showed a marked increase in oxidative stress in liver and kidney homogenate, as determined by lipid peroxidation. Histopathological observation of the liver sections of alloxan-induced diabetic mice showed several lesions including cellular vacuolization, cytoplasmic eosinophilia and lymphocyte infiltrations, but with individual variability.Treatment of diabetic mice with propolis extracts results in decreased number of vacuolized cells and degree of vacuolization; propolis treatment improve the impairment of fatty acid metabolism in diabetes. Renal histology showed corpuscular, tubular and interstitial changes in alloxan-induced diabetic mice. Test components did not improve renal histopathology in diabetic mice. Conclusions: Propolis preparations are able to attenuate diabetic hepatorenal damage, probably through its anti-oxidative action and its detoxification proccess as well as the potential to minimize the deleterious effects of free radicals on tissue. The protective role of propolis against the ROS induced damages in diabetic mice gives a hope that they may have similar protective action in humans.
Alexandria Science Exchange Journal
Monosodium glutamate (MSG) is used to enhance the flavour in the preparation of food to improvement the palatability. However, it induces oxidative stress and causes many health complications. This work determines the effect of MSG on kidney and liver functions and to know the protective role of propolis in male rabbits. Twenty rabbits were divided into four equal groups. Group (1) used as control, group (2) received propolis (8mg/kg body weight), group (3) received MSG (50mg/kg bodyweight) and group (4) received propolis (8mg/kg body weight) plus MSG (50mg/kg bodyweight) in the same time as combination group. The doses was given every other day for 12 weeks. Monosodium glutamate significantly elevated the concentration of thiobarbituric acid reactive substances (TBARS) and significantly decreased the concentration of reduced glutathione (GSH) and antioxidant enzymes (Catalase(CAT), Superoxide dismutase (SOD), Glutathione S-transferase (GST), Glutathione peroxidase (GPx)) in plasma, kidney and liver. Activities of liver enzymes (lactate dehydrogenase (LDH), aspartate transaminase (AST), alkaline phosphatase (AlP), gama-glutamyl transferase (GGT), acid phosphatase (AcP) and alanine transaminase (ALT)), creatinine, glucose and urea were enhanced significantly, while globulin, albumin and total protein were reduced in plasma. Also, treatment with MSG resulted in the deterioration of the histological architecture in liver and kidney. The combination group alleviated its adverse effects on liver and kidney, and this protection might be due to the antioxidant properties of propolis. In addition, the histology of liver and kidney results were supported by the biochemical findings. From the obtained results, it could be concluded that propolis capable to mitigate the oxidative stress, hepatotoxicity and nephrotoxicity induced by MSG in male rabbits.
The Antioxidant Potential of Saudi Propolis Extract on Hepatorenal Toxicity in Mice
Indian Journal of Animal Research, Volume 58 Issue 8: 1311-1318 (August 2024)
Background: In advanced cirrhotic conditions, hepatorenal syndrome detrimentally affects renal function. Interest has grown in propolis for its cytoprotective properties against various exogenous agents. This study evaluates the efficacy of Saudi propolis extracts in mitigating hepatorenal toxicity induced by carbon tetrachloride in mice. Methods: Thirty-two male Swiss Albino mice were divided into four groups: a Control (-) group receiving distilled water; a Control (+) group subjected to intraperitoneal CCl4 at 0.5 mL/kg (20% v/v in corn oil) on day 6; a Standard group treated daily with silymarin at 200 mg/kg and a group given an oral dose of aqueous propolis extract (APE) at 8.4 mg/kg. Result: Histological and biochemical analyses confirm propolis extract’s role in preventing hepatocyte apoptosis and reducing inflammatory infiltrates in kidney tissues, improving the histological appearance of hepatic and renal tissues with fewer fibrotic changes. The application of immunohistochemistry, along with reductions in anti-apoptotic proteins such as BCL-2 and p53, supports these findings, highlighting the antioxidant potential of Saudi propolis extracts in addressing hepatorenal toxicity.
Veterinary World, 2021
Background and Aim: Propolis has a protective effect against cellular damage caused by toxic agents such as drugs, metals, xenobiotics, and chemicals. The aim of this study was to investigate the antioxidant activity and the effect of ethanolic extract of propolis on carbon tetrachloride (CCl4)-induced oxidative stress on kidney and liver injury in rat. Materials and Methods: The study quantified phenol, flavone, and flavonol in propolis and assessed antioxidant activity using 2, 2-diphenyl-1-picrylhydrazyl, ferric reducing antioxidant power, and molybdate. The investigators used four groups of rats to study the effect of propolis on CCl4-induced toxicity. Propolis extract was given orally (500 mg/kg) for 12 days, and CCl4 (1 mL/kg) was administered intraperitoneally on day 5 of the experiment. Blood and tissue samples of the liver and kidney were collected on day 13 to measure biochemical and oxidative parameters. The parameters included malondialdehyde (MDA), protein carbonyl formation (PCO), advanced oxidation protein products (AOPP), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and ascorbic acid (AA). Biochemical parameters included liver enzymes, blood urea (BU), creatinine, and uric acid (UA). Results: CCl4 decreased antioxidant agents, including CAT, GPx, GSH, and AA in the liver and kidney tissues. The oxidative agents' levels, including MDA, PCO, and AOPP, increased by CCl4 compared to the control group. CCl4 increased liver enzymes, UA, BU, and creatinine in the blood samples. Propolis significantly alleviated liver and kidney function, improved antioxidant parameters, and decreased levels of oxidative agents. Conclusion: The data showed for the 1st time that Moroccan propolis has a protective effect against CCl4-induced kidney and liver toxicity by maintaining the activity of the antioxidant defense system, which was most likely due to its antioxidant activity.
Ameliorative effect of propolis against methoxychlor induced hepato renal dysfunction
2013
The present study has determined the ability of Methoxychlor (MXC), an organochlorine pesticide, to induce hepatoxicity and nephrotoxicity in female rats and the effectiveness of propolis in modulating these effects in liver and kidney of rats. Animals were assigned to 1 of 4 groups in each stage of the experiment for 6 and 12 months, respectively: control; 200 mg MXC/kg bw, twice / week, orally; 200 mg propolis/L drinking water/ daily; MXC (200 mg/kg bw, twice / week, orally) plus propolis (200 mg/L, drinking water), respectively. Rats were administered their respective doses for 6 or 12 months. The levels of serum enzymes and histological alterations in liver and kidney were investigated. In addition, the levels of lipid peroxidation metabolite thiobarbituric acid reacting substances (TBARS) and the antioxidant enzyme reduced glutathione (GSH) were assayed in liver homogenate. MXC caused a significant increase in serum transaminases (AST and ALT), alkaline phosphatase, urea and cr...
Journal of Ethnopharmacology, 2006
Propolis is a resinous substance produced by honeybees that possesses many biological activities, such as antitumor, antioxidant, antimicrobial, anti-inflammatory, and immunomodulatory, among others. The purpose of the present study was to investigate the biochemical profile of propolistreated rats to observe whether propolis might lead to side effects after administration. Three different treatments were analyzed: (1) rats were treated with different concentrations of propolis (1, 3 and 6 mg/kg/day) during 30 days; (2) rats were treated with 1 mg/kg/day of ethanolic or water extracts of propolis (EEP, WEP) during 30 days; (3) rats were treated with 1 mg/kg/day of ethanolic extract of propolis during 90 and 150 days. Our results demonstrated no alterations in the seric levels of cholesterol, HDL-cholesterol, total lipids, triglycerides and in the specific activity of aminotransferases (AST) and lactic dehydrogenase (LDH) of propolis-treated groups when compared to controls. On the basis of our findings, since propolis does not induce any significant change in seric parameters, it is claimed that long-term administration of propolis might not have any cardiac injury.
Advances in Therapy, 2007
Propolis is a natural product produced by bees that was discovered through the study of traditional cures and knowledge of indigenous people throughout the world. It is rich in vitamins A, B, C, and E, and in amino acids, copper, iron, manganese, and zinc. The investigators studied the duration-dependent hepatoprotective effects of propolis extract (200 mg/kg, orally) against carbon tetrachloride (CCl 4 ; 1.5 mL/kg, intraperitoneally)-induced liver damage in rats. Administration of CCl 4 caused a sharp elevation in the activity of serum transaminases and serum alkaline phosphatase. A significant depletion in hepatically reduced glutathione was observed with significantly enhanced hepatic lipid peroxidation. After CCl 4 administration, glycogen contents and activities of alkaline phosphatase, adenosine triphosphatase, and succinic dehydrogenase were significantly decreased, whereas total protein contents and activity of acid phosphatase were increased in the liver and kidney. Propolis extract reversed alterations in all parameters when administered within 6, 12, and 24 h of toxicant exposure. Propolis therapy produced duration-dependent protection, with maximal protection achieved at 24 h after CCl 4 exposure. It is believed that propolis in its natural form has general pharmacologic value and marked hepatoprotective potential because of its composition of minerals, flavonoids, and phenolic compounds.
Nutrition & Metabolism, 2022
Background The present systematic review is conducted, focusing on the existing evidence of Propolis's effects due to its various health benefits, mainly antioxidant and anti-inflammatory properties on preserving renal function. Methods A systematic search of PubMed, Scopus, Embase, ProQuest, and Google Scholar was undertaken for relevant papers published from the start until January 2021. Results This review revealed that Propolis affects fasting blood sugar (FBS), postprandial blood glucose, advanced glycation end products (AGEs) concentrations, malondialdehyde (MDA) levels, urinary concentrations of reactive oxygen metabolites (Tbars), total oxidant status (TOS), oxidative stress index (OSI), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) formation favorably. The findings on hemoglobin A1C (HbA1C), insulin, homeostasis model assessment of insulin resistance (HOMA-IR), β-cell function (HOMA-β), quantitative insulin sensitivity check index (QUICKI), and lipid profile were controversi...
2013
The chemical content of two types of propolis was investigated, along with its effect on body weight gain, relative organs weight, liver function and lipid profiles for 8 weeks on albino rats. Ethanolic extract of Egyptian and commercial propolis samples were investigated by capillary GC-MS. The compounds were characterized by comparison with library searches. Fifty seven compounds from Egyptian propolis were identified; while Forty four compounds from commercial were identified. The ethanol-propolis extract, at doses of 500, 1000, 1500 mg/kg body weight/day, was given, by gavage, to male rats for 8 weeks. At the end of the treatment, body weight gain, relative organs weight, plasma lipid profiles levels and liver function parameters were measured. No significant effects happened in all tested groups compared with control and each other in body weight gain, relative organs weight, lipid profiles and liver function parameters. We suggest that both tested samples safe and could use in human nutrition.
Prophylactic Effect Of Aqueous Propolis Extract Against Acute Experimental Hepatotoxicity In Vivo
Zeitschrift für Naturforschung C, 2002
Propolis has been extensively used in folk medicine for the management of a wide spectrum of disorders. In a previous study, we demonstrated the protective effect of the aqueous propolis extract (APE) against the injurious effects of carbon tetrachloride (CCl 4 ) on hepatocytes in vitro. The present investigation was carried out to show whether the hepatoprotective effect of the extract could also be manifested in vivo. Rats were given APE orally for 14 consecutive days, before being subjected to a single intraperitoneal injection of CCl 4 . One day after the CCl 4 injection, the animals were sacrificed, hepatocytes were isolated and liver homogenates were prepared for the assessment of liver injury. In isolated hepatocytes, APE afforded protection against CCl 4 -induced injury as manifested by a decrease in the leakage of the cytosolic enzyme lactate dehydrogenase (LDH), decreased generation of lipid peroxide and maintenance of cellular reduced glutathione (GSH) content. In principle, similar findings were observed in liver homogenates. The present findings show that APE has in vivo hepatoprotective potential which could be attributed at least in part to the maintenance of cellular GSH content. The latter effect seems to play an important role in conserving the integrity of biomembranes as it was associated with a decrease in lipid peroxidation and reduced leakage of cytosolic LDH.