Zonulin and I-FABP are markers of enterocyte damage in celiac disease (original) (raw)
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Эффективная фармакотерапия, 2022
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Курский научно-практический вестник «Человек и его здоровье», 2019
Цель исследования-определить эффективность культуральной жидкости аллогенных гепатоцитов и выделенных из них белков при коррекции иммунных и метаболических нарушений остро возникшего ишемического поражения печени. Материалы и методы. Исследование выполнено на 105 половозрелых и здоровых крысах линии Вистар. Острое ишемическое поражение печени (ОИПП) вызывали пережатием гепатодуоденальной связки в течение 20 минут. Выделенные от новорожденных животных аллогенные гепатоциты использовали для приготовления культуральной жидкости, из которой получали белки. Культуральную жидкость аллогенных гепатоцитов (КЖАГ) и их белки вводили с началом моделирования острой ишемии печени пятикратно (с интервалов в 24 часа) внутрибрюшинно крысам с ОИПП по расчету 5 мг/кг белка. Результаты. Острая ишемия печени вызывает развитие биохимических синдромов поражения печени (цитолиза, внутри-и внепеченочного холестаза, токсического поражения, недостаточности синтетических процессов), активирует процессы перекисного окисления липидов, нарушает внутриэритроцитарный метаболизм, вызывает развитие оксидантного стресса, супрессию формирования адаптивного и врожденного иммунитета. Введение животным с ОИПП КЖАГ, неразделенных белков КЖАГ или белков КЖАГ с молекулярной массой (ММ) менее 130 кДа нормализует или корригирует изученные биохимические и иммунологические параметры. Белки КЖАГ с ММ больше 130 кДа такими эффектами не обладают. Заключение. При анализе корригирующей активности КЖАГ или белков КЖАГ при остро возникшем ишемическом поражении печени на динамику биохимических маркеров, формирование клеточного и гуморального иммунитета, внутриэритроцитарный метаболизм, изменение метаболической и функциональной активности нейтрофилов периферической крови, активирование свободно-радикального окисления, установлена следующая последовательность (по степени увеличения эффективности): отсутствие корригирующего эффекта у белков КЖАГ с ММ более 130 кД, наличие нормализующей и корригирующей активности: КЖАГ-неразделенные белки КЖАГ-белки КЖАГ менее 130 кД. Ключевые слова: иммунометаболические нарушения; белки культуральной жидкости аллогенных гепатоцитов; остря ишемия печени.