Rapid UV-Vis spectroscopy methods for quantification of ranitidine tablets (original) (raw)
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ORIENTAL JOURNAL OF CHEMISTRY , 2020
Ranitidine is a histamine-2 receptor blocker and it is effective against peptic ulcer, gastroesophageal reflux disease and heart burn. The main objective of this study was to develop and validate an easy, affordable and cost-effective method for the determination of ranitidine in tablet dosage form. The development and validation study was performed under the guidance of ICH and USP. Results showed that the proposed validated method has good accuracy with % RSD of 0.60. Repeatability and intermediate precision suggested good precision whereas the value of correlation coefficient 0.9999 confirmed about the linearity of the method. The system suitability data and similarity factors were also found within the permissible range. The specificity study revealed that there was no placebo and diluent effect on the absorbance. Further, stability study of analytical solutions as well as estimation of drug content from market products were also performed.
Ranitidine lies under the group of drugs called H2 blockers. Ranitidine reduces the quantity of acid produced by the stomach. It’s used in the treatment and prevention of ulcers in stomach and in intestine like GERD & Zollinger-Ellison syndrome. Ranitidine is also coupled with enhance the risk of developing pneumonia. Assay was done by different brands available in market of ranitidine. Different dilutions were made and check out their linearity and regression was found to be linear. Absorbance was measured by UV Spectrophotometer and statistically one way ANOVA was implemented to analyze the comparison b/w groups and within groups.
A rapid, simple and sensitive spectrophotometric method has been developed for the determination of ranitidine hydrochloride in pharmaceutical pure and dosage forms. The method depends on the charge-transfer complex formation between ranitidine bases as n-electron donor with chloranil as-acceptor to give a colored complex that absorbs maximally at 550 nm. Beerís law is obeyed in the concentration ranges 2-40µg/mL with molar absorptivity of 2.510 4 L mole-1 cm-1. The proposed method is precise, accurate and specific for the quantitative determination of drug in bulk and dosage forms. The results of analysis of commercial formulations and the recovery study (standard addition method) of ranitidine suggested that there is no interference from any excipients, which are present in pharmaceutical formulations of ranitidine. Statistical comparison of the results was performed with regard to accuracy and precision using studentís ttest and F-ratio at 95% confidence level. There is no significant difference between the reported and proposed methods with regard to accuracy and precision.
International Journal of Scientific Research in Science and Technology, 2019
A Simple, selective, precise and rapid absorption correction Spectrophotometric method has been developed and validated as per ICH guideline for the simultaneous estimation of Ranitidine Hydrochloride and Dicyclomine Hydrochloride in tablet dosage form. Method is base on UV Spectrophotometric for determination of two drug, by using Methanol as a solvent and diluted the same with 0.1N NaOH, solution. In this UV method, the two wavelength were selected, 311.4 nm and at 217 nm for RANTD and DICY, respectively. this method was validated according to ICH guideline and Linearity range, was found to be 7.5-37.5 µg/ml and 1-5 µg/ml for RANTD and DICY, respectively. The method was successfully applied to assay drugs in tablet.
Simultaneous Estimation of Naproxen and Ranitidine HCl by Using UV Spectrophotometer
2008
The development of a UV Spectrophotometric method for simultaneous estimation of Ranitidine HCl and Naproxen involves absorbance measurement of Ranitidine HCl at 313 nm in pH 7.4 phosphate buffer and 314 nm in both 0.1N HCl and in water and that of Naproxen at 229 nm in pH 7.4 phosphate buffer and 232 nm in both 0.1N HCl and in water corresponding to the respective absorption maxima. Both the drugs obey Beer-Lambert's law in the range of 5-25 µg/ml for Ranitidine HCl and 0.2-1.25 µg/ml for Naproxen. The method developed was validated to determine its linearity, precision, reproducibility and sensitivity. The tablet formulations were evaluated for the percent content of both the drugs at the selected wavelengths and the percent potency were 98.83 and 99.15 for Naproxen and Ranitidine HCl respectively.
European Journal of Chemistry
A simple, sensitive and reproducible method for the determination of ranitidine hydrochloride in pharmaceutical preparations was investigated. This spectrophotometric method was based on the formation of a deep red color product with ninhydrin in basic media and the absorbance measured at λmax = 480 nm. The reaction occurs at 45 °C with pH = 10 having a contact time of 38 minutes. Under the optimum conditions, Beer’s Law is obeyed in the concentration range of 8.98×103 - 9.90×104 µg/L. The coefficient of correlation was found to be 0.999 for the obtained method with molar absorptivity of 3.05×103 L/mol.cm. The calculated Sandell’s sensitivity is 0.108 μg/cm2. The limit of detection and limit of quantification are 0.0997 and 0.3023 µg/mL, respectively. The low values of the percentage relative standard deviation and percentage relative error indicate the high precision and the good accuracy of the proposed method. The stoichiometry of the reaction is determined and found to be 1:4 (Ran...
2014
Ranitidine is a common drug used as antacid. In the present study, method (under International Conference of harmonization (ICH) Guidelines) for the validation of Ranitidine in its formulation (Zantac 300mg), was developed spectrophotometrically at max λ = 314 nm. The medium of dissolution was water, at 30C and 37C. The concentration of the drug from 1 to 35 ppm was prepared and the regression of the curve was Y = 0.031x − 0.014, 2 R = 999.The percentage of relative standard deviation (RSD) 75.3, limit of detection (LOD) 0.47, limit of quantization (LOQ) 1.57. Method detection limit (MDL) value 0.47 (t-value 1.56) was determined. The calculated values of Sandells sensitivity and the molar absorptivity were 0.00301384 2 / / g ml cm μ and 6 1 1 4.4 10 M cm − − × respectively. The average recovery of the solution (average of two results of recovery) was made to 99.82%. The label claim was made from the average of two verification test as 100. The solution was stable up to 24 hours, and...
A simple and sensitive spectrophotometric method is described for the determination of ranitidine hydrochloride (RNH) in pharmaceuticals. It is based on the formation of colored condensation product with p-dimethylaminobenzaldehyde (PDAB) followed by measurement of absorbance at 503 nm. The absorbance was found to increase linearly with the concentration of the drug and formed the basis for quantification. The calibration graph was linear from 50.00 to 350.00 μg mL -1 . The apparent molar absorptivity is calculated to be 0.311×10 4 L mol -1 cm -1 and the calculated sandell's sensitivity is 0.1132 µg cm -2 . The limits of detection and quantification are found to be 0.00346 and 0.0105 µg mL -1 respectively. The procedure is used to determine ranitidine hydrochloride in pharmaceutical products. The associated pharmaceutical materials did not interfere.
Journal of Chromatography A, 1999
The H receptor agonist pharmaceuticals containing ranitidine hydrochloride and famotidine are widely used to inhibit 2 gastric acid secretion. A high-performance thin-layer chromatographic method was developed for their in-process control and content uniformity testing. HPTLC separation was performed on silica precoated plates using the USP 23 mobile phase for famotidine and toluene-methanol-diethylamine (9:1:1, v / v) for ranitidine. The samples were applied on a HPTLC plate automatically. Quantification was done by densitometry at in situ UV absorption maxima of ranitidine hydrochloride and famotidine at 320 nm and 276 nm, respectively. The method was validated in terms of selectivity (related compounds and placebo effect), system suitability, range (30 to 230 ng for ranitidine hydrochloride and 80 to 580 ng for famotidine), accuracy, precision, ruggedness and analyte stability. A large number of analyses were performed simultaneously with a low solvent consumption. The method is fast, accurate and cost-effective.
Journal of AOAC International
An accurate and simple kinetic method is described for the determination of ranitidine and nizatidine in pure form and in pharmaceuticals. The method is based on the reaction of the compounds with 7-chloro-4-nitrobenz-2-oxa-1,3-diazole in pH 7.4 borate buffer at 60 degrees C for a fixed time of 25 min for both compounds. The absorbance of the reaction product is measured at 495 nm for ranitidine and nizatidine. Calibration graphs were linear over the concentration range of 2-20 microg/mL, with limits of detection of 0.13 (3.7 x 10(-7) M) and 0.25 microg/mL (7.5 x 10(-7) M) for ranitidine and nizatidine, respectively. The proposed method was applied successfully to the determination of ranitidine in tablets and ampoules with average recoveries of 100.26+/-0.69 and 100.29+/-0.59%, respectively, and to the determination of nizatidine in capsules with an average recovery of 104.26+/-0.44%. The results obtained are in good agreement with those obtained by the other methods used for compa...