Thermoresponsive Membrane Based on Thermotropic Liquid Crystalline Cholesteryl - (L-lacticacid)n System: Study of Its Drug Permeability (original) (raw)
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Iranian Journal of Medical Microbiology, 2016
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Optics and Photonics Society of Iran, 2015
In this paper laser ablation of aluminum target in distilled water was used to produce aluminum oxide (alumina) nanoparticles. Nanosecond pulsed laser beam (τ~10ns and λ=1.06μm) with fixed fluence of 46 J/cm2 was used to generate the nanoparticles in this experiment. The effect of ageing of nanoparticles in particle size characteristics was investigated. Scanning electron microscopy (SEM) together with image processing technique was used to evaluate the results. The experimental results showed that size distribution of the nanoparticles can be influenced by ageing due to Ostwald ripening process.
Journal of Mazandaran University of Medical Sciences, 2016
Background and purpose: Platelets communicate with different immune cells and can activate B-lymphocytes and induce the production of antibodies from these cells. Platelet microparticles (MPs) originate from platelets and express the surface markers of platelets. This study aimed at investigating the ability of these microvesicles on production of antibodies from B-lymphocytes. Materials and methods: In this experimental study, platelet MPs were isolated from platelet concentrates and B cells were isolated from human whole blood. Then MPs were co-cultured with Blymphocytes. In different days of culture, the production of IgG antibodies was studied in the supernatants of culture medium using ELISA method. The results were analyzed by paired-samples t-test. Pvalue < 0.05 was considered statistically significant. Results: Platelet MPs stimulate the production of antibodies by B-lymphocytes. During 5-day coculture, significant increase was observed in the production of IgG antibodies...
Iranian Journal of Medical Microbiology, 2019
Article Subject: Molecular Microbiology DOI: Background and Aims: High prevalence of Methicillin Resistant Staphylococcus Aureus isolates (MRSA) as well as the multi-drug resistance in this bacterium causes difficulties in the treatment of infections due to these bacteria. Hence, detection of MRSA isolates by rapid and accurate methods is necessary. PCR-ELISA is an accurate and molecular technique that is used for the detection of several pathogens. The aim of this study is the detection of MRSA using PCR-ELISA. Materials and Methods: Specific primers for mecA gene were designed. Then, dNTP labeled with Digoxigenin was applied for amplifying mecA gene. DIG-labeled PCR products were seeded on the well coated streptoavidin and identified by anti-DIG-peroxidase conjugate. Furthermore, Biotin-labeled DNA probe specific for mecA gene was used. Sensitivity and specificity of the method was determined. Resistance to methicillin among 70 clinical isolates was determined by the disk diffusion, agar dilution and PCR-ELISA methods. Results: MecA gene of S. aureus was amplified using gene specific primers resulted in a fragment with 310 bp length. Findings from the PCR-ELISA technic showed no cross-reactivity with Klebsiella Pneumoniae, Bacillus subtilis and Esheriashia coli as control bacteria and its sensitivity was 0.5 ng. The prevalence of MRSA clinical isolates by the disk diffusion, agar dilution and PCR-ELISA methods was 60%, 58.5% and 60%, respectively. Conclusion:The PCR-ELISA technique was known as an accurate and rapid test for the detection of infection agents using their specific gene. This technic can applied as an appropriate alternative method for time-consuming, less sensitive and expensive techniques such as Real-time PCR and differential biochemical tests which are currently used in laboratory.
Evaluation of Antibacterial Properties of Electrospun Polyurethane-chitosan Nanofiber Media
Journal of Occupational Hygiene Engineering
Background and Objective: The accumulation of airborne bioaerosols on filtration media and their gradual proliferation in the presence of appropriate moisture and environmental conditions is one of the major problems against using these media. The use of hybrid media containing antibacterial agents is one of the available solutions to this problem. The present study aimed to fabricate nonwoven nanofiber media with an antibacterial activity using an electrospinning process. Materials and Methods: Polyurethane-chitosan nanofiber media the weight ratios of 100 to 0, 90 to 10, 80 to 20, and 70 to 30 were fabricated by simultaneous electrospinning process. The evaluation of the antibacterial properties of the media was performed after their preparation by standard methods of disk diffusion (ISO 20645) and colony counting (ISO 20743). Results: The investigation of antibacterial activity of samples by both methods showed that the media with polyurethane to chitosan weight ratio of 70 to 30 had suitable antibacterial activity. The mean values of bacterial growth inhibition zone and antibacterial activity for polyurethane-chitosan (70/30) media were obtained at 0.26 and 2.225 mm, respectively, indicating the significant antibacterial activity of this media. Conclusion: The results showed that antibacterial nanofiber media can be created by adding chitosan nanofibers as antimicrobial agents to the polyurethane nanofiber.