Assessment of the Agglutination Test for the Identification of Staphylococcus Aureus Isolates (original) (raw)
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Comparison of five agglutination tests for identification of Staphylococcus aureus
Journal of clinical microbiology, 1997
Various commercially produced agglutination kits are widely used for the identification of Staphylococcus aureus. These kits detect the presence of protein A and/or clumping factor on S. aureus. The literature has shown that methicillin-resistant S. aureus (MRSA) isolates which are deficient in both clumping factor and protein A may be misidentified. Two products, Slidex and Staphaurex Plus, utilize specific anti-S. aureus antibodies, potentially giving them greater sensitivity compared to products without these antibodies. We report a prospective study designed to compare the performance characteristics of Fastaph, Slidex, Staphaurex, Staphaurex Plus, Staphyloslide, and the tube coagulase test for the identification of staphylococcal isolates. All discrepant isolates were tested with the Gen-Probe AccuProbe S. aureus test and were identified to the species level with conventional reference biochemicals. A total of 1,193 isolates were tested, including 33 MRSA and 423 methicillin-se...
Evaluation of Three Slide Agglutination Tests for Rapid Identification of Staphylococcus aureus
Acta Veterinaria Scandinavica, 1991
Three slide agglutination tests for identification of Staphylococcus aureus were compared. The agglutination tests used for evaluation were Staphaurex (Wellcome Diagnostics), Staphyslide-Test (BioMerieux), and ANI S. aureus TEST (Ani Biotech Oy). A total of 347 isolates were analyzed, including 288 strains of S. aureus, 49 of S. epidermis, 11 of S. intermedius, 12 strains of other staphylococci and 14 non-staphylococcal strains. One hundred of the S. aureus strains were isolates from cases of food poisoning, 129 from mastitis and 59 from other clinical cases. The sensitivities of the tests were also compared using diluted suspensions of S. aureus strains and with purified Protein A dilutions. The results showed that the sensitivities of the tests were 98.6 %, 97.9 % and 99.0 % for Staphaurex, Staphyslide-test and ANI S. aureus TEST, respectively. The specificities were 100 % for the Staphyslide test and 98.8 % for both the ANI S. aureus TEST and the Staphaurex test. The sensitivitie...
Journal of Clinical Microbiology, 2001
A newly marketed rapid agglutination kit for the identification of Staphylococcus aureus , Slidex Staph Plus (bioMérieux), was compared to Staphaurex Plus (Murex Diagnostics) and Pastorex Staph-Plus (Sanofi Diagnostics Pasteur). The study took place in three clinical microbiology laboratories in three different European countries. A total of 892 staphylococcal isolates, including 278 methicillin-sensitive S. aureus (MSSA) isolates, 171 methicillin-resistant S. aureus (MRSA) isolates, and 443 coagulase-negative staphylococcal isolates, were analyzed. The sensitivities (MSSA/MRSA) and specificities, respectively, were 98.2% (98.9%/97.1%) and 98.9% for Slidex Staph Plus, 98.2% (98.2%/98.2%) and 96.2% for Staphaurex Plus, and 98.7% (98.6%/98.8%) and 95.7% for Pastorex Staph Plus. The specificity of the Slidex Staph Plus kit was statistically significantly higher than the specificities of Staphaurex Plus and Pastorex Staph-Plus. The Slidex Staph Plus is a very reliable test for the iden...
Journal of Medical Microbiology, 2006
Most routine laboratory detection of Staphylococcus aureus isolates is based on rapid agglutination test systems. Failure of agglutination assays to identify meticillin-resistant S. aureus strains (MRSA) has been demonstrated. The aim of this study was to evaluate six commercially available agglutination tests for the detection of meticillin-sensitive S. aureus (MSSA) and mecA-positive MRSA strains. The Dry Spot Staphytect Plus H test (Oxoid), the Pastorex Staph Plus H test (Bio-Rad), the Slidex Staph-Kit H and Slidex Staph Plus H test (bioMé rieux), the Staphaurex Plus H test (Remel) and the Staphylase Test H (Oxoid) were used. As determined by pulsed field gel electrophoresis, 52 distinct MRSA strains from five countries, 83 MSSA strains and 150 coagulase-negative staphylococci were included. Species identification and determination of susceptibility patterns were performed using colony morphology, Gram stain, catalase testing, tube coagulase testing, DNase testing, mannitol fermentation, susceptibility testing towards oxacillin by Etest H , coagulase gene PCR, fibrinogen receptor gene PCR and PCR of the mecA gene. Sensitivity of the agglutination tests ranged from 82?7 to 100?0 % for MRSA strains and 92?8 to 100?0 % for MSSA strains, respectively. Specificity of the test systems ranged from 91?3 to 99?1 %. None of the six agglutination assays produced correct reactions for all staphylococci tested. Only the Dry Spot Staphytect Plus H test correctly identified all 52 MRSA strains. For the other tests kits, sensitivity of MRSA detection was lower than for MSSA isolates. Depending upon the local MRSA prevalence and the parameter of interest (sensitivity or specificity), these test systems may be useful for routine diagnostic purposes.
European Journal of Clinical Microbiology & Infectious Diseases, 2010
In this study, we evaluated a fourth-generation agglutination assay (Staph Plus; DiaMondiaL[DML]) for the rapid identification of Staphylococcus aureus. First, comparison with three third-generation assays (Slidex Staph Plus, bioMérieux; Staphaurex Plus, Murex Diagnostics; Pastorex Staph-Plus, Sanofi Diagnostics Pasteur) was performed on a predefined strain collection: 265 coagulase-negative staphylococci (CNS), 266 methicillinresistant S. aureus (MRSA) and 262 methicillin-susceptible S. aureus (MSSA) strains ("strain study"). Second, patient material-derived strains (883 CNS, 847 MSSA and 135 MRSA) were tested concurrently with both the DML and Slidex assays ("daily practice study"). In the strain study, the overall sensitivity and specificity of the DML, Slidex, Staphaurex and Pastorex assays were 99.2% and 100%, 98.1% and 100%, 95.2% and 100%, and 98.2% and 98.8%, respectively. Using the respective tests, the result was indeterminate in 0.0%, 0.6%, 0.4% and 1.5% of the strains. Overall, the sensitivity of the DML and Slidex assays were comparable in both sub-studies. However, in MRSA strains, the sensitivity of the DML assay was significantly lower than the Slidex assay. The specificity of the Slidex assay was significantly higher than the DML assay. However, the percentage of indeterminate results was much higher for the Slidex than the DML assay. In conclusion, the presumptive identification of S. aureus by the DML assay proved to be equal to third-generation latex agglutination assays. Recently, the Staph Plus Latex Kit (DiaMondiaL [DML], Sees, France), a new latex agglutination assay for the identification of S. aureus, has been marketed. In order to
American Journal of Internal Medicine
Bacterial colonies can differ greatly in their morphologies. These differences can help us in identifying different species of bacteria. The clinical isolates of Staphylococcus Aureus are subjected to standard biochemical tests, to observe the biochemical characteristics. One hundred and ten (110) pathogenic Staphylococcus Aureus strains were used in this study. Characteristics of these strains were determined by biochemical tests such as catalase, coagulase, DNase test, test for beta haemolysin, fermentation of mannitol and lactose. Staphylococcus Aureus [American Type Culture Collection (ATCC) 25923] was used as a reference control organism. From this study, males were more infected than females, having 60 (61.8%) and 42 (38.2%) respectively. The age group with the highest number of isolates was (0-10) years while blood culture had the highest frequency of Staphylococcus Aureus isolates with a frequency of 42 (38.1%). It was also observed that 100% of Staphylococcus Aureus showed positive results in catalase and tube coagulase, about 101 (91.8%) were positive for slide coagulase production test, 102 (92.7%) strains were positive to DNase test, 63 (57.3%) were positive to beta haemolysin while 79 (71.8%) and 106 (96.4%) strains fermented Mannitol and lactose respectively. This study reveals that no single test that can be used for the identification of Staphylococcus Aureus, hence the need for sequel testing using proper biochemical tests should be used for the identification of Staphylococcus Aureus isolates.
Staphylococcus aureus: Prevalence among normal healthy individuals
Nepal Journal of Medical Sciences, 2013
Background: To determine the prevalence of Staphylococcus aureus among normal healthy individuals in relation to age, gender and site of isolation. Methods: A cross sectional study was conducted among healthy students and staff volunteers between the age group 15-65 years from Gulf Medical University (GMU) and Gulf Medical College Hospital and Research Centre (GMCH&RC), Ajman. Aseptically collected nasal and throat swabs were processed for direct Gram stain Microscopy and cultured on appropriate media. Based on the growth on Mannitol Salt Agar and tube coagulase test Staphylococcus aureus and Coagulase negative Staphylococci (CoNS) were grouped. Results: Of the 127 voulnteers screened 67 were from GMU and 60 from GMCH&RC. From 49/127 (38.5%) volunteers 124 Staphylococci isolates were isolated. Of which 62 (50%) were Staphylococcus aureus and 62 (50%) were CoNS. Among the 62 Staphylococcus aureus 35 (56.45%) were from nasal swab and 27 (43.54%) were from throat swab. Of the 62 CoNS 4...
Journal of Advances in Medicine and Medical Research, 2018
Background: Staphylococcus aureus is one of the most common pathogen isolated. Accurate and rapid identification of S. aureus and MRSA is of great importance for proper care of the patient. Keeping this in mind, we had used Pastorex Staph plus rapid agglutination test to confirm S. aureus. Aim: To evaluate Pastorex Staph plus rapid agglutination test for confirming Staphylococcus aureus isolated from clinical specimens. Study Design: Cross-sectional type. Methodology: This study was conducted in a tertiary care hospital in Eastern India. It included all clinical samples, where Staphylococcus spp. was isolated. The phenotypic confirmation of the Original Research Article
Journal of Medical Sciences, 2015
Objective: To evaluate performance (sensitivity & specificity) of a range of phenotypic tests which are currently used in tertiary care hospitals for the indentification of S aureus and to identify an optimal phenotypic test that is reliagle and cost effective and can be used with confidence for confirmation of S. aureus.Material and Methods: The present study was conducted at clinical microbiology laboratory department of Pathology of Northwest General Hospital and Research Centre, Peshawar from January 2012 to September 2013. The study group consisted of 300 samples were collected from different clinical sources i.e. patient’s blood, body fluids, pus swabs, wound swabs, urine and sputum. During sampling safety methods were adopted and data were collect regarding age, sex, types of specimens and present health condition were also recorded.Results: All clinical samples evaluated with slide coagulase (SCT), mannitol salt fermented test (MSA) and DNase test the result were 95%, 87% an...
Assessment of the Coagulase Test in the Identification of Staphylococcus Aureus Strains
2019
Introduction: Staphylococcus aureus are among the most important and several pathogens in human infections. The objectives of this study are to evaluate the efficacy of the coagulase test in the detection of Staphylococcus aureus in the laboratory and to identify the factors associated with Staphylococcal infections. Methods: It is a prospective study of 69 strains of Staphylococci in a period of six months from January 2019 to June 2019 in the University Hospital of Befelatanana. Results: Among of the 69 strains of Staphylococci, 47 (68%) were represented by Staphylococcus aureus. Concerning the prediction performance of the catalase test, It has a sensitivity of 93.6%, a specificity of 100%, a positive predictive value (PPV) of 100% and a negative predictive value (NPV) of 88%. Concerning the associated factors, the men (71.1%) (p=0.46; NS), the patients aged 60 and over (76.5%) (p=0.63; NS), in resuscitation department (93.3%) (p=0.01) and with suppuration (100%) (p=10-3) were th...