Synovial fluid gelatinase concentrations and matrix metalloproteinase and cytokine expression in naturally occurring joint disease in horses (original) (raw)
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Le Centre pour la Communication Scientifique Directe - HAL - Diderot, 2000
Early detection of osteoarthritis in horses represents a challenge for equine practitioners. Several biological markers have been implicated in the pathological processes involved in articular cartilage destruction. To further document cartilage matrix proteases production, synovial fluid was collected from 14 horses (90 joints) before they were subjected to euthanasia. Growth macroscopic examination of the joints gave information on cartilage alterations. Samples were analyzed for matrix metalloproteinase (MMPs) activities by gelatin zymography and tumor necrosis factor alpha (TNF-α) cytotoxicity using L929 cells. Significant increase of MMP-9 monomer and dimer were found in synovial fluids of joints with severe cartilage alterations. On the contrary, the activity of TNF-α was not correlated to the degree of joint damage. The levels of MMP-9 monomer and dimer in the synovial fluid could reflect cartilage alteration in arthritis in the horse. horse / osteoarthritis / synovial fluid / metalloproteinase / tumor necrosis factor alpha Résumé-Activité des métalloprotéinases et du tumor necrosis factor-alpha dans le liquide synovial de chevaux : corrélation avec les altérations du cartilage articulaire. Le dépistage précoce des arthropathies reste un défi pour le vétérinaire équin. Certains marqueurs biologiques présents dans le liquide synovial pourraient être des indicateurs précoces de l'ostéoarthrose. C'est pourquoi, le liquide synovial de 90 articulations de 14 chevaux devant être euthanasiés a été recueilli. La dissection des articulations prélevées a ensuite permis de visualiser les surfaces articulaires. Pour chaque échantillon, l'activité des métalloprotéinases (MMPs) a été évaluée par zymographie et la cytotoxicité du tumor necrosis factor alpha (TNF-α) a été mesurée sur culture cellulaire. La corrélation des
Annals of the Rheumatic Diseases, 1998
Objective-To investigate the influence of age, osteoarthritis (OA), and osteochondrosis (OC) on the matrix metalloproteinase (MMP) activity in the synovial fluid (SF) of equine joints. Methods-SF was collected from normal and osteoarthritic metacarpophalangeal joints (normal: 14 adult, 28 juvenile; OA: 22 adult). And from normal and osteochondrotic tarsocrural joints (5 months: 11 normal, 8 OC; 11 months: 7 normal, 6 OC). Subsequently, overall MMP activity was measured. Results-The level of active MMPs was almost twofold higher in SF from juvenile horses (age up to 11 months) than in SF from mature animals (4-30 years; p<0.001). In juvenile horses MMP activity was higher in 5 month old foals than in 11 month old foals (p<0.01). In adult horses MMP activity was independent of age. In OA joints the activity was nearly twice as high as in normal joints (p<0.001). In OC joints MMP activity was not significantly diVerent from normal, age matched, control joints. Conclusions-MMP activity in SF from normal adult joints is not related to age. In juvenile joints MMP activity is significantly higher than activity in joints from adult animals. It is hypothesised that the gradual decrease in MMP activity with increasing age reflects the declining metabolic activity resulting from ceasing growth and the accompanying decrease in cartilage remodelling. The increased MMP activity in osteoarthritic joints most likely reflects matrix destruction. In osteochondrosis MMP mediated matrix degradation appears not to be diVerent from normal joints.
Prospective Biochemical Markers for Osteoarthritis in Horses
2014
A central feature of the osteoarthritis (OA) involves erosive destruction of the articular cartilage extracellular matrix (ECM) on the surfaces of joints. The resultant loss of joint function makes studies on mechanisms underlying ECM degradation critical for treatment of the disease and prevention of disability. In the present study synovial and serum samples were collected from normal (n=20), Early OA (n=10) and Late OA (n=20) adult male horses attended from surgery clinic, faculty of veterinary medicine Cairo University. The activities of different types of matrix metalloproteinases (MMPs) and tissue inhibitor metalloproteinases (TIMPs) in synovial fluid were estimated by Substrate and Reverse zymography respectively and the expression of MMP-13 was determined by Western blot. The level of procollagen type IIC-propeptide (PIICP) in serum and synovial fluid was measured by ELISA. The activity of pro and active form of MMP-9 and level of PIICP was significantly increased in early s...
Changes in Synovial Fluid Biomarkers after Experimental Equine Osteoarthritis
Journal of veterinary research, 2017
The study aimed to clarify the changes in the concentration of inflammatory mediators, proteases, and cartilage degradation biomarkers in the synovial fluid of joints in an equine osteoarthritis model. Osteoarthritis was induced in eight Mongolian horses by a sterile intra-articular injection of amphotericin B, which was injected into the left carpal joint in a dose of 2 mL (25 mg/mL). The control group comprised five horses which were injected with an equal dose of sterile physiological saline into the left carpal joint. Synovial fluid was obtained at baseline and every week after injection. Test methods were based on ELISA. In the course of the osteoarthritis, the concentration of biomarkers in joint synovial fluid showed an increasing trend. IL-1, IL-6, MMP-9, MMP-13, ADAMTS-5, CS846, GAG, HA, CTX-II, and COMP concentrations sharply increased before the onset of significant symptoms of lameness, whereas TNF-α, MMP-2, and MMP-3 concentrations rose sharply after the occurrence of s...
Journal of Equine Veterinary Science, 2015
The aim of this study is to evaluate the effect of pamidronate on the clinical score and the secretory profile of inflammatory biomarkers (IL-6, TNFα, MMP-2 and MMP-9) in the synovial fluid in clinically healthy horses and in horses with joint disease. Healthy horses and horses with joint symptoms were examined and subjected to a standardised clinical evaluation of the locomotor system. The clinical condition was evaluated by a global score. MMP-2 and MMP-9 were measured by gel zymography. The concentration of cytokines (IL-6 and TNF-α) in synovial fluid was determined by ELISA. Pamidronate treatment significantly improved the clinical condition of horses with osteoarthritis (OA). Values of IL-6 (pg/ml) were similar (ns) in the healthy control group (102.2±26.94) and at day 3 of treated group (TD3) (113.9±18.33). TNF-α level, at day 3 of treatment, was significantly lower in treated groups than in untreated osteoarthritis (UOA). TD group registered a fast increase in MMP-9 activity but till day 21 and 60 it was not detectable. No significant differences were found in the MMP-2
Serum level of cartilage oligomeric matrix protein (COMP) in equine osteoarthritis
Equine veterinary journal, 2002
This study was designed to assay and compare cartilage oligomeric matrix protein (COMP) in horse sera, in samples from normal and joint diseased horses, and to investigate the relationships between COMP in sera and synovial fluids (SF) with keratan sulphate (KS) data. Sera from 38 horses free of any joint pathology (controls) and from horses with aseptic joint disease (AJD horses, n = 40) were assayed forCOMPand KS concentrations. Of the 78 horses in the study, 53 were also assayed for COMP and KS concentrations in SF. COMP and KS were measured by inhibition ELISA, using monoclonal antibodies 12C4 and 5D4, re s p e c t i v e l y. The COMP concentration in sera from AJD horses (mean ± s.d. 10.7 ± 7.4 µg/ml) was significantly (P<0.02) lowerthan in control sera (14.8 ± 7.8 µg/ml). The joint disease sera also had significantly lower (P<0.01) KS levels (180.5 ± 61.8 ng/ml) than controls (237.1 ± 116.1 ng/ml). A significant correlation (r= 0.52, n = 53, P<0.001) was seen between serum and SFin COMPlevels; no such relationship was seen in KS levels. It is possible that serum COMPconcentration could be a more specific marker of equine joint disease than any other described to date.