Phenolic Extract of Seagrass, Halophila ovalis Activates Intrinsic Pathway of Apoptosis in Human Breast Cancer (MCF-7) Cells (original) (raw)
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Cellular and Molecular Life Sciences, 2006
The effects of different marine derived agents were studied in A549 cell growth. These drugs induced cell cycle arrest at the G 2 -M phase associated with the up-regulation of GADD45α−γ and down-regulation of c-Myc. In treated cells, GADD45α−γ and c-Myc were up-and down-regulated, respectively. A cascade of events leading to apoptotic mitochondrial 'intrinsic' pathway was observed in treated cells: (1) dephosphorylation of BAD serine 136 ; (2) BAD dissociation from 14-3-3 followed by its association with BCL-XL; (3) cytochrome c release; (4) caspase-3 activation, and (5) cleavage of vimentin. Caspase(s) inhibitor prevented the formation of cleavage products and, in turn, apoptosis was inhibited through a p53-independent mechanism. Moreover, these compounds did not activate NF-κ B. Our findings may offer new insights into the mechanisms of action of these agents in A549 cells. The better understanding of their effects might be important to fully exploit the potential of these new drugs.
Bioactive Compounds of Seaweeds and Their Effects on Certain Types of Cancer
Avicenna Journal of Medical Biochemistry
Cancer is considered as one of the major health problems worldwide. So far, no completely effective method has been found for cancer treatment. Therefore, the rise of using natural products has been proposed as an alternative therapy in this regard. For many years, the seaweed has been a source of many functional bioactive compounds including polysaccharides, polyphenols, pigments, terpenes, and many others. These compounds have shown many bioactivities including anticancer activity against different kinds of cancer. Bioactive compounds obtained from the seaweed have been demonstrated to cause apoptosis in cancer cells and trigger cell cycle arrest with low cytotoxicity against normal cells. In this review, it was attempted to shed light on the anticancer activity of some seaweed-derived bioactive compounds.
Nutrition and Cancer, 2019
Recent evidence provides that seafood has a lot of health benefits due to its unique bioactive compounds. Sea squirt is widely cultured and consumed as a foodstuff in Korea; however, seldom reports with reference to bioactivities are available until now. In this study, edible part of sea squirt was hydrolyzed by pepsin and its hydrolysates was evaluated for anticancer effect on human colon cancer HT-29 cells. Sea squirt hydrolysates (SSQ) reduced HT-29 cell viability. Treatment with SSQ resulted in the increase in reactive oxygen species (ROS) generation followed by disruption of mitochondrial membrane potential (MMP). Flow cytometry analysis revealed that SSQ induced G2/M phase arrest and apoptosis evidenced by Hoechst 33342 staining. Levels of mRNA expression by real-time polymerase chain reaction (PCR) showed that treatment with SSQ in HT-29 cells upregulated expression of p53, bax, and caspase-3 genes and downregulated expression of bcl-2 gene. Protein level of cytochrome c into cytosol and caspase-3 by Western blotting were also increased by treatment with SSQ in HT-29 cells. These results suggest that SSQ may be useful for functional food ingredients and/or nutraceuticals.
International Journal of Pharmaceutical Sciences and Drug Research
Seagrasses are one of the groups of marine angiosperms widely distributed all over the coastal regions. The seagrass ecosystem is very productive and traditionally used for a variety of therapeutic purposes. The present study aimed to investigate the in-vitro anticancer activity against malignant melanoma (A375), lung carcinoma (A549), cervix adenocarcinoma (HeLa) and colorectal adenocarcinoma (HT29) cell lines and phytochemical screening of ethyl acetate fraction of Halodule uninervis (EAF) from Mandapam coastal region, Rameswaram, Gulf of Mannar, India. The qualitative phytochemical analysis of EAF was performed as followed by standard methods. EAF showed the presence of alkaloids, steroids, terpenoids, flavonoids, phenols and quinones. The total flavonoid and phenolic contents of EAF were determined using the spectrophotometric method. The total flavonoid and phenolic contents showed 67.33 ± 1.52 mg QE/g dried fraction and 87.75 ± 1.39 mg GAE/g dried fraction, respectively. Gas C...
Sustainable Marine Structures
Seaweed is an enormous resource comprised with natural bioactive compounds comprise with several therapeutic effects including anticancer activity. In this context, the biochemical composition of seaweeds plays a major role. Many biochemical compounds extracted from seaweeds and crude extracts, such as aqueous, methanolic account mainly for the anticancer effect against several cancer cell line. In this contrast seaweed extracts inhibit cancer cell growth and proliferation by inducing apoptosis and inhibit metastasis activity. In this review, biochemical and anticancer properties of seaweeds are discussed and this will provide the basic information to develop a novel chemotherapeutic drug to challenge the cancer.
Natural products as inducers of apoptosis: Implication for cancer therapy and prevention
Apoptosis is a programmed cell death and a highly organized physiological mechanism to destroy injured or abnormal cells. Apoptotic cells exhibit remarkable morphological features and characteristic molecular expression. Apart from physiological stimuli there are exogenous factors which can contribute to induce apoptosis. The induction of apoptosis in tumour cells is considered very useful in the management and therapy as well as in the prevention of cancer. A wide variety of natural substances have been recognized to have the ability to induce apoptosis in various tumour cells of human origin. These substances are compounds with different chemical entities and many of these are present in plants with medicinal value and in various fruits and vegetables commonly consumed by humans. Mode of action in the apoptotic pathway of some of these compounds has been delineated.
Journal of Applied Biomedicine, 2016
Developing plant derived chemopreventive agents that can divert the carcinogenic process and inhibit tumour progression may greatly reduce serious health consequences of cancer. This study investigated the antiproliferative and apoptosis inducing ability of Rubus fairholmianus root acetone (RFRA) on MCF-7 cells. RFRA treatments showed an increase in the in vivo antioxidant levels such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione (GSH) in mice. The extract showed significant antitumour activity with 76.57% increase in life span of ascites tumour bearing mice and 1.94 cm 3 [ 1 _ T D $ D I F F ] reductions in solid tumour volume at 250 mg/kg. The cytotoxicity spectrum analysis indicated that RFRA is a potent cytocidal agent with varying levels of toxicity (IC 50 : 57.14-29.36 mg/ml). A significant dose dependent decrease in viability, proliferation and increase in cytotoxicity, caspase 3/7 activity were observed in MCF-7 cells. RFRA (20 mg/ml) induced apoptosis in MCF-7 cells, which was observed by morphology; DNA ladder formation and increased apoptotic population in flow cytometry analysis. These findings strongly suggest the use of R. fairholmianus as natural antioxidant with profound antitumour activities. It can act as a potent cytotoxic agent, which is able to induce apoptosis in MCF-7 cells via caspase 3/ 7 upregulation.
journal-mhr.com
THE AIM OF THIS REVIEW is to provide an updated overview of experimental in vitro and in vivo investigations focusing on the anticancer activity of few herbs specially phytochemicals from daily kitchen ingredients. Potential use of these natural agents in cancer therapy and chemoprevention is also discussed. Studies using animal models and cultured human malignant cell lines have demonstrated antitumor and cancer preventive activities of many herbs and their main ingredients. Possible mechanisms of few of these (e.g., thymoquinone from nigella sativa, curcumin from turmeric, and organosulfur compounds from garlic) along with their biological activities are discussed here.
The edible red seaweed Eucheuma cottonii is abundantly cultivated for carrageenan production. This study investigated the effects of dietary E. cottonii polyphenol-rich extract (ECME) on breast cancer. In vitro assays showed that ECME was antiproliferative against oestrogen-dependent MCF-7 and oestrogen-independent MB-MDA-231 human breast-cancer cells (IC 50 values of 20 and 42 lg/ml, respectively) but was non-toxic to normal cell lines. The ECME (150 and 300 mg/kg BW) was fed to female rats and, after 4 weeks, rat mammary tumour was induced using LA7 cells (inoculated subcutaneously). The ECME inhibited tumour development and erythrocyte lipid peroxidation in the cancer-induced rats, dose-dependently. It showed anti-oestrogenic effects on the rat estrous cycle and serum hormone levels. Electron microscopy and histopathology observations confirmed apoptosis in the rat mammary tumours. The polyphenol-rich ECME was tumour-suppressive via apoptosis induction, downregulating the endogenous oestrogen biosynthesis, and improving antioxidative status in the rats.
Gene, cell and tissue, 2022
Background: Breast cancer is the most lethal cancer causing death among women, especially in developed countries. Using medicinal plants and natural resources to control and prevent different types of cancer in human has been the focus of attention. Objectives: The current study aimed to investigate the possible effects of hydroalcoholic extracts from some plant species such as Lavandula angustifolia, Ephedra major, and Scenedesmus obliquus on cell viability of two breast cancer MCF7 and SK-BR3 cell lines. Methods: MTT assay and gene expression analysis were performed adopting qRT-PCR method. Target cells were treated with different concentrations of plant extracts in a 24-hour period. Data were analyzed using Graphpad Prism version 8.3.0. Differences among means were determined using Student's t-test or one-way ANOVA with Dunnett's Multiple Comparison Test. Results: An indirect relationship was detected between extract concentration and cell viability. The result of the cell viability (IC-50) showed that the extract of S. obliquus had the maximum effect on both tested cells at concentrations above 100 µg/mL. A high cell death rate was observed in MCF-7 cell line compared to SK-BR3 cell line 24 h after the treatment. According to results from gene expression analysis, Caspase 3 gene increased in all treated cells, while the bcl-xl gene expression declined in the tested cells. Conclusions: It was concluded that the extracts from three plants had both positive and dose-related effects on MCF-7 and SK-BR3 cell line. However, it was recommended that future complementary studies should be conducted in order to evaluate the cell viability effect of these plants on human cancer.