Correlation of frequency of spermatozoa morphological alterations with sperm concentration in ejaculates of Polish Landrace boars (original) (raw)
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Journal of Veterinary Research
Introduction Determination of sperm concentration and morphology in ejaculate is essential in evaluating fertility. Sperm shape and dimensions may depend on their concentration in ejaculate. The aim of the study was to evaluate the dependence of Hypor boar morphological and morphometric sperm characteristics on concentration in ejaculate. Material and Methods The study was conducted on 120 ejaculates from 12 Hypor boars. Depending on sperm concentration, they were put into three groups: low, medium, and high. Ejaculate sperm concentration was determined with the photometric method. Slides were prepared from semen samples which were evaluated for the morphology of spermatozoa. The preparations for morphological analyses were by the eosin-gentian staining method. Results As the sperm concentration in the ejaculate increased, the length, perimeter, and area of the sperm heads also did. In the ejaculates with higher sperm concentration the sperm heads were rounder. The ratios of head di...
Annals of Animal Science
Reproductive efficiency in pigs is largely dependent on the fertility of the boar. Boars used at insemination stations should produce a large amount of semen with high fertilization capacity. The sperm count influences the conception rate and the number of insemination doses produced. The aim of this study was to evaluate the morphological and morphometric characteristics of boars of the Hypor breed in relation to the total sperm count in the ejaculate. An analysis was performed of 120 ejaculates collected from 12 Hypor boars used at three insemination stations. The ejaculate sperm count was found to affect the morphometric characteristics of spermatozoa from boars. In the ejaculates with the lowest sperm count the spermatozoa heads were significantly shorter and narrower and had a smaller surface area. The sperm from ejaculates with the lowest sperm count had relatively small head dimensions in relation to the tail length and total sperm length. In the ejaculates with the most sper...
Ejaculate traits and sperm morphology depending on ejaculate volume in Duroc boars
Journal of Veterinary Research
The aim of this study was to evaluate the dependence between ejaculate traits, sperm morphology, and ejaculate volume in Duroc boars.The analysis involved 121 ejaculates collected from 12 Duroc boars kept in three artificial insemination centres located in central Poland. Ejaculates were collected manually at one-month intervals, over a period of 10 months. At least 10 ejaculates were collected from each boar. The material was divided by ejaculate volume and each ejaculate was assigned to one of three volume groups: 160 mL and lower, 161–200 mL, and 201 mL and higher. The ejaculates were assessed to identify the basic physical traits and determine the incidence of morphological abnormalities in the spermatozoa, specifying major and minor abnormalities. Furthermore, the morphological structure indices for the spermatozoa were also calculated.In large-volume ejaculates, spermatozoa were more elongated in shape, their heads were more elongated and had the largest flagella. With an incr...
Roczniki Naukowe Polskiego Towarzystwa Zootechnicznego, 2018
The aim of the research was to determine the dependence of physical properties of boar ejaculates on the time interval between successive ejaculate collections. The material for the study comprised 1954 ejaculates collected manually from boars used for artificial insemination. For all ejaculates, the ejaculate volume, sperm concentration, percentage of motile spermatozoa, and number of spermatozoa in the ejaculate were determined, and the number of insemination doses per ejaculate was calculated. The boars’ suitability for artificial insemination was evaluated taking into account the effect of the time passed between ejaculate collections. The ejaculate volume and the concentration and number of sperm in the ejaculate were shown to increase with the time elapsed since the previous ejaculate. The number of insemination doses obtained per ejaculate increased as well. Ejaculates with a high sperm count were obtained when the ejaculation frequency was no more than every 4 days. The perc...
The effect of breed, boar and season on some properties of sperm
Biotehnologija u stocarstvu, 2013
The main objective of this study was to assess the phenotypic variability of the ejaculate volume (VOL) and progressive sperm motility (MO) under the influence of breed, boar within breed and season. The study included 555 ejaculates of Danish Landrace (DL), Danish Large White (DLW) and Danish Duroc (DD) boars. The effect of boar was analyzed within the investigated breeds. The impact of the season was investigated as the influence of the month in which the ejaculate was taken. The model included a linear regression effect of boar age when the semen was taken. Data analysis was performed using the statistical package SAS 9.1.3 (SAS Inst. Inc.., 2002-2003). Impact assessment was carried out using the GLM procedure. The impact of all factors analyzed in both sperm traits was significant to highly significant, and the observed factors largely explain the phenotypic variability VOL compared to MO. The highest values were found in VOL of DL boar (3:58 ? 164.96 ml), and the MO of boar bre...
2005
A statistical approach using sequentially principal component analysis (PCA), clustering, and discriminant analyses was developed to identify sperm morphometric subpopulations in well-defined portions of the fresh boar ejaculate. Semen was obtained as 2 portions (the first 10 mL of the sperm-rich fraction and the rest of the ejaculate, respectively) and frozen using a conventional protocol. Before freezing, an aliquot was used for computerassisted sperm morphometry analysis (ASMA). Postthaw quality was evaluated using computer-assisted sperm analysis (CASA), and an annexin-V/PI assay evaluated sperm membranes. The PCA revealed that 3 variables represented more than 78% of the cumulative variance in sperm subpopulations. The clustering and discriminant analyses, based on 5780 individual spermatozoa, revealed the ex-istence of 4 sperm subpopulations. The relative percentage of these subpopulations varied between boar and ejaculate portions. Linear regression models based on measured morphometric characteristics could account for up to 36% of the percentage of intact sperm membranes postthaw. The ASMA protocol used in our study was useful to detect subtle morphometric differences between spermatozoa, and the combination of this analysis with a multivariate statistical procedure gave new information on the biological characteristics of boar ejaculates that is not given by conventional sperm analysis.
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2016
The study aimed at analysing the effects of ejaculate volume on the physical parameters of ejaculates and the sperm morphology of Hypor boars. The analyses involved 114 ejaculates collected from 12 Hypor insemination boars. The ejaculates were classified according to the criterion of ejaculate volume. Three groups were specified: ejaculates with a volume of 251 ml or lower (Group I), ejaculates with a volume of 252-310 ml (Group II), and ejaculates with a volume of 311 ml or higher (Group III). The ejaculates were assessed to identify the basic physical traits and determine the incidence of morphological abnormalities in the spermatozoa, specifying major and minor abnormalities. Furthermore, the morphological structure indices for the spermatozoa were also calculated. Rising ejaculate volume accompanied with a rise in the total number and motility of spermatozoa, and a simultaneous slight fall in sperm concentration in the ejaculates. The ejaculates with the highest volumes turned out to contain more morphologically well-formed spermatozoa. We also determined that rising ejaculate volume is accompanied with increasing sperm dimensions, especially those of the head. The increased parameters were the length and the width of sperm heads, as well as their perimeters and areas. Ejaculate volume has an impact on the shape of Hypor boar spermatozoa. As the ejaculate volume increases, the shape of sperm heads becomes increasingly more oval. Additionally, spermatozoa in ejaculates with greater volumes have larger heads in relation to flagellum length.
The Dynamic of Main Indices of Spermiogram at Boars, in Function on Some Factors of Influence
Breeding boars used for semen production. This is conditioned by many factors. The research aim was to assess the some indices of spermogram (volume, mobility, concentration, total sperm / ejaculate) at 21 boars, the breeds Large White, Landrace, Duroc and Pietrain in relation with some factors of influence: the race, age, individual and harvesting season. The age of boars was between 8 months and 30 months. The rhythm of harvesting was following: one harvesting followed by the five days break. Depending on the breed, were differences for volume (183,5± 4,88 ml at Pietrain boars and 256±5,3 ml at Landrace boars) and for concentration (309,7±6,0 millions/ml at Duroc boars and 430,7±9,3 millions/ml at Pietrain boars). In function on the season, semen volume varied insignificant between summer (minimum values: 188.4 ± 5.6 ml) and winter (maximum values: 222.1 ± 6.6 ml). The spermatozoon concentration was insignificantly influenced by the season (371.6 ± 11.7 million/ml -summer; 399.4 ±...
Sperm Morphology and Fertility in Artificial Insemination Boars
The objective of this study was to investigate quality of semen and to determine the relationships between semen quality and fertility in the different pig breeds. Twelve boars were randomly selected at Joint stock company "Lekėčiai", Lithuania. Quality of 47 ejaculates was assessed and reproductive data from 209 sows were included into the analysis. None of initial semen quality parameters showed significant correlation with non-return rate % or litter size. Only some of semen quality traits (morphological defects) of the spermatozoa-loose abnormal heads, short broad, big head and acrosomal defects correlated significantly wiht non-return rate % and the litter size. Semen quality parameters differed significantly between the breeds. Danish Landrace boars, compared to the boars of Duroc breed, had lower incidence of pathological spermatozoa in their semen (P < 0.001), but higher percentage of sows conceived. Keywords: boar, semen, non-return rate, litter size. KUILIŲ SP...
Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura, Alimentaria, Piscaria et Zootechnica
Streszczenie. W niniejszej pracy podjęto próbę oceny międzyrasowej i wewnątrzrasowej zmienności ejakulatów oraz morfologii, wymiarów i kształtu plemników knurów inseminacyjnych. Podstawowe cechy ejakulatów cechuje relatywnie duża zmienność wewnątrzrasowa i międzyrasowa. Ejakulaty knurów ras Landrace i Large White mają wyraźnie większą objętość niż ejakulaty knurów rasy Duroc oraz mieszańców Duroc × Pietrain, a ejakulaty knurów rasy Landrace zawierają więcej plemników niż ejakulaty knurów innych ras. Plemniki knurów mieszańców wykazują mniejszą ruchliwość niż plemniki knurów innych ras. W ejakulatach knurów wszystkich ras stwierdzono bardzo małą frekwencję plemników ze zmianami głównymi i podrzędnymi. Frekwencję zmian morfologicznych plemników cechuje bardzo duża zmienność wewnątrzrasowa, przy stosunkowo małej zmienności międzyrasowej. Wymiary morfometryczne plemników cechuje mała zmienność wewnątrzrasowa, przy relatywnie dużej zmienności międzyrasowej. Plemniki knurów rasy Landrace mają wyraźnie dłuższe witki oraz większe pole powierzchni główki niż plemniki knurów innych ras.