Fast oligonucleotide deprotection phosphoramidite chemistry for DNA synthesis (original) (raw)

1990, Tetrahedron Letters

Abstract

sparkles

AI

A new set of base protecting groups for cyanoethylphosphoramidite nucleosides has been developed to reduce post-synthesis deprotection times in DNA synthesis. The introduction of dimethylformamidine protecting groups significantly decreases deprotection times to 1 hour at 55°C or 8 hours at room temperature, coupled with high resistance to depurination. This advancement allows for rapid synthesis of high-quality oligonucleotides, enabling effective polymerase chain reactions and improving overall efficiency in oligonucleotide production.

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References (13)

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