A search for double minute chromosomes in cultured lymphocytes from different types of tumors (original) (raw)

1995, Cancer Genetics and Cytogenetics

Abstract

Double minutes are considered the products of DNA amplification and are rare in normal human cells. They have been observed in cultured lymphocytes in selected samples of human populations as one of the characteristics of the so-called rogue cells. We scored 9500 metaphases of cultured lymphocytes from 65 subjects with a variety of heredity and sporadic tumors and from 30 healthy subjects. The 15 cells with double minutes were found in subjects with multiple endocrine neoplasia type 1 (14 cases) and with familial adenomatous polyposis (1 case). Only one rogue cell was found among the 15 cells with double minutes.

Loading...

Loading Preview

Sorry, preview is currently unavailable. You can download the paper by clicking the button above.

References (11)

  1. Wintersberger E (1994): DNA amplification: new insights into its mechanism. Chromosoma 103:73-81.
  2. Wright JA, Smith HS, Watt FM, Hancock MC, Hudson DL, Stark GR (1990): DNA amplification is rare in normal human cells. Proc Natl Acad Sci USA 87:1791-1795.
  3. Bloom AD, Neel JV, Tsuchimoto T, Meilinger K (1973): Chro- mosomal breakage in leucocytes of South American Indians. Cytogenet Cell Genet 12:175-186.
  4. Hsu TC (1983): Genetic instability in the human population: a working hypothesis. Heroditas 98:1-9.
  5. Taw'an El, Cartmel CL, Pyta EMT (1985): Cells with multiple chromosome aberrations in control individuals. Mutat Res 144:247-250.
  6. Awa AA, Neel IV (1986}: Cytogenetic "rogue" cells: what is their frequency, origin, and evolutionary significance? Proc Natl Acad Sci USA 83:1021-1025.
  7. Neel JV, Awa AA, Kodama Y, Nakano M, Mabuchi K (1992}: "Rogue" lymphocytes among Ukrainians not exposed to radio- active fall-out from the Chernobyl accident: the possible role of this phenomenon in oncogenesis, teratogenesis, and muta- genesis. Proc Natl Acad Sci USA 89:6973-6977.
  8. Madhavi R, Guntur M, Ghosh R, Ghosh PK (1990): Double min- ute chromosomes in the leukocytes of a young girl with breast carcinoma. Cancer Genet Cytogenet 44:203-207.
  9. Scappaticci S, Maraschio P, del Ciotto N, Fossati GS, Zonta A, Fraccaro Iv[ (1991): Chromosome abnormalities in lymphocytes and fibroblasts of subjects with multiple endocrine neoplasia type 1. Cancer Genet Cytogenet 52:85-92.
  10. Schimke RT, Sherwood W, Hill AB, Johnston RN (1986): Over- replication and recombination of DNA in higher eukaryotes: potential consequences and biological implications. Proc Natl Acad Sci USA 83:2157-2161.
  11. Stark GR, Debatisse M, Giulotto E, Wahl GM (1989): Recent prog- ress in understanding mechanism of mammalian DNA amplifi- cation. Cell 57:901-908.