Determination of Xanthine and Uric Acid in Xanthinuric Urine and Extracellular Fluid of Porcine Endothelial Cells of the Pulmonary Artery by High Performance … (original) (raw)

Analytical methods are critical for the measurement and separation of purines like xanthine and uric acid in biological fluids. Various chromatographic techniques, including high-performance liquid chromatography (HPLC) and gas-liquid chromatography, are employed to enhance the detection and quantitative analysis of these metabolites in samples such as urine and extracellular fluids from porcine pulmonary artery endothelial cells. The study delves into the specifics of method selection, emphasizing the importance of retention time, mobile phase composition, and method optimization to enable reliable and accurate results in purine metabolite analysis.