COMPARATIVE EFFICACY OF KOROLLA (MOMORDICA CHARANTIA) EXTRACT AND IVERMEC ® POUR ON WITH THEIR EFFECTS ON CERTAIN BLOOD PARAMETERS AND BODY WEIGHT GAIN IN INDIGENOUS CHICKEN INFECTED WITH ASCARIDIA GALLI (original) (raw)
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Aqueous and ethanol extracts of four indigenous medicinal plants namely neem (Azadirachta indica),pan (Piper betle), papaya (Carica papaya\ and korolla (Momordica charartia) were evaluated in aitro anthelmintic activity on adult Ascaridia galli. The research work was conducted in the Department of Parasitology, Bangladesh Agricultural University, Mymensingh during the period from July, 06 to November, 06. Evaluation of freshly prepared aqueous extract of five plant materials revealed in oitro wormicidal properties and the ethanol extract of selected plant materials showed almost similar results against adult Ascaridia galli. A significantly higher efficacy was recorded in 1% aqueous extract of korolla (whole) and 2h aqueous extract of neem leaves. Ethanol extract of korolla (whole) and neem leaves also showed higher efficacy but not significantly different from others. The results show that active anthelmintic ingredients are more concentrated in korolla (whole) than neem leaves. The aqueous solutions of korolla (whole) and neem leaves preparation have significantly increased anthelminthic activity than that of alcoholic extracts. Both of these plant preparations could easily be prepared by farmers. By following the accurate dose of aqueous extract solution of korolla (whole) and neem leaves, the farmers of our country can cheaply control the adult A. galli infection in indigenous chickens.
The study was conducted to explore the effect of two herbal drugs, Caesalpinia crista and Nigella sativa in comparison with Oxfendazole in broilers experimentally induced with Ascaridia galli infection. A total of 130 day old broiler chicks were divided into five groups i.e. A, B, C, D and E each group having 26 birds. Group E was kept as negative control, while all other groups were induced with experimental Ascardia galli infection on 15 th day. Group A was kept as positive control and groups B and C were treated with Caesalpinia crista and Nigella sativa as methanolic extract orally once @50mg/kg body weight and group D was medicated with Oxfendazole @10mg/kg body weight orally once on 25 th day post infection. On 25th day post infection before treatment 4 birds from each group were slaughtered, and worm count was done. The efficacy was confirmed by counting the worm, slaughtering all the bird on 30 th day post infection. The mean worm count was significantly different (p<0.05) between the treated and non-treated groups. The efficacy of the plant extracts were less as compare to Oxfendazole. The efficacy of Caesalpinia crista and Nigella sativa was 70.83% and 57.5% respectively while the efficacy of Oxfendazole was 90.83%. The efficacy of drugs was calculated on the basis of reduction in total worm count after treatment.
Veterinary World, 2019
Aim: Intestinal parasites cause severe diseases at poultry farms, especially in developing countries, including Indonesia. Infections cause significant decreases in productivity at poultry farms. Ascaridia galli is a common nematode found in chickens with a prevalence of up to 92%. This study evaluates the in vitro and in vivo effects of Areca catechu crude aqueous extract (AAE) as an anthelmintic against A. galli. Materials and Methods: In the in vitro study, already-infected slaughtered chickens were collected from local slaughterhouses in Yogyakarta. The chicken intestines were carefully examined and transported to the Parasitological Laboratory of Gadjah Mada University, Yogyakarta. A. galli was collected from the intestines and put into a Petri dish containing 0.62% saline solution. Sixty A. galli individuals were soaked in different concentrations of the AAE for 3-6 h. The number of deceased A. galli was recorded by ascertaining whether they had ceased movement using a stereo microscope. The morphological characteristics of the parasites were studied. The in vivo study used 40 female chickens that were 1.5 months old and infected with the eggs of A. galli. Approximately 3000 infective eggs were found in each chicken. There were four treatment groups; Group A served as the control and was treated with Aquadestilata, Group B was treated with 26 mg/ml of AAE, Group C was treated with 79 mg/ml of AAE, and Group D was treated with 50 mg/ml of pyrantel pamoate. The AAE was applied using a pipette for 14 days. The observed parameters include the effect of the treatments on hematology, body weight (BW), and the number of A. galli eggs within each gram of fecal matter. Results: The AAE damaged the morphology, including the anterior end, posterior end, and vulva, of A. galli in vitro. The tegument of the anterior and posterior ends was slightly rougher, wrinkled, and damaged. The vulva and spicule ducts were irregular and indistinct. The average eggs per gram (EPG) in the untreated control increased in vivo from 1450±355.90 to 1975±325.96. The average EPG decreased from 1485±386.62 to 0±0.00 during 14 days of treatment of 79 mg/ml of AAE. The mean BW gain in the treated chickens was significantly (p<0.5) higher than the control. The treatment with 79 mg/ml of AAE increased the total erythrocyte count, total leukocyte count, hemoglobin, and packed cell volume values in the chickens. Conclusion: AAE can cause morphological changes and subsequent death in adult A. galli in vitro and effectively decrease ascariasis severity in chickens, therefore increasing overall BW in vivo.
INTERNATIONAL JOURNAL OF BIOSCIENCES, 2021
Limited substantiations are available supporting the pharmacological properties of herbal plants utilized in ethnoveterinary medication which remained sustainable in local communities in spite of advancements in animal health today. This study evaluated through in vitro and in vivo anthelmintic assays the folkloric use of Chrysophyllum cainito and Psidium guajava, which are among the selection of documented florae in the Philippines being used in ethno-veterinary medicine. In vitro anthelmintic evaluation showed time-dependent and concentration-dependent efficacies. The ovicidal action of Chrysophyllum cainito bark ethanolic extract at 60mg/ml has recorded 94.65% inhibition capacity, whereas Psidium guajava bark ethanolic extract generated 92.64% and 96.28% efficacies at 30mg/ml and 60mg/ml dilutions, respectively. The wormicidal activity of the former elicited 88.88% at 60mg/kg, while the latter yielded 88.88% and 94.44% mortalities to worms at 30mg/ml and 60mg/ml dilutions, correspondingly. Probit analysis on the lethal concentration (LC50) against eggs and worms was logged at different magnitudes for both plants. In vivo assessment by means of fecal egg count reduction (FECR) rate has signified biologically, that the tested plants undoubtedly possess anthelmintic property.
Acta Veterinaria Hungarica
The antiascarid activity of Caesalpinia crista Linn. seeds, popularly known as Karanjwa, was evaluated in chickens of the Fumi breed, suffering from artificially induced Ascaridia galli infection. Eggs per gram (EPG) counts were determined in the droppings of chickens prior and after treatment with powdered C. crista at doses of 30, 40 and 50 mg/kg of body weight along with its extracts in water and methanol in amounts representing 50 mg/kg of crude powder. The crude drug at the dose rates of 40 and 50 mg/kg and its methanol extract induced a significant (P < 0.001) effect on post-treatment days 10 and 15 while the 30 mg/kg dose was efficacious (P < 0.05) on day 15 only. However, the aqueous extract did not show significant results. These results suggest that a 50 mg/kg dose of C. crista seed powder, its equivalent methanolic extract and piperazine (200 mg/kg) are equieffective in treating the ascarid infection of poultry. The crude C. crista powder appears to be potent and sa...
Beni-Suef University Journal of Basic and Applied Sciences, 2018
Pumpkin seeds (Cucurbita pepo) and Pomegranate peel (Punica granatum) have anthelmintic properties. The aim of this study was to compare the anthelmintic efficacy of pumpkin seeds ethanolic extract and pomegranate peel aqueous extract against Ascaridia galli in vitro and in vivo in Baladi chicks. On adult worms, the extracts of the two herbs were compared in vitro at concentrations of 25, 50, and 75 mg/ml with fenbendazole at a concentration of 5 mg/ml. Chicks were infected with Ascaridia galli eggs containing second stage larva and treated with 2000 mg/ kg of each of the extracts compared with 100 mg/kg fenbendazole. In vitro, all concentrations of pumpkin seed extract and the concentration of 75 mg/ml pomegranate peel extract exhibited a nearly similar effect to fenbendazole. In vivo, the mortality rate of the worms extracted from the 2000 mg/kg pumpkin seeds extract-treated chicken was non-significantly different from that of fenbendazole for 48 h. While pomegranate peels extract exhibited a lower lethal effect than fenbendazole. The anthelmintic efficacy was dependent on time and concentration. The study presented the anthelmintic efficacy of the pumpkin seeds and pomegranate peel extracts on Ascaridia galli. Pumpkin seed extract was more effective than pomegranate peel extract. Future studies to determine the optimal dose to maximize their effectiveness especially for pumpkin seeds as anthelmintic therapeutic are required.
Efficacy of neem leaf extract against ascariasis in indigenous chicken
2014
The efficacy of a medicinal plant neem (Azadirachta indica) leaves and a reference anthelmintic (piperazine) was evaluated against adult Ascaridia galli in chicken in vitro and in vivo. The aqueous extracts of neem leaves and a rage of piperazine concentrations were screened for their anthelmintic effects against Ascaridia galli in indigenous chicken. The data revealed that the aqueous extract of neem leaves showed significant anthelmintic efficacy at a dose rate of ranging from 1 mg/ml, 2 mg/ml, 4 mg/ml and 20 mg/ml against the chicken ascariasis. Shortest time of parasite paralysis was observed at dose rate 20mg/ml. Piperazine was incubated at the dose rate of 0.1, 0.2, 0.4, 0.8 and 4mg /ml to adult worms. Shortest time of parasite paralysis was observed at dose rate 4mg/ml. Using the extract of neem leaves at a dose of 1gm/kg body weight (bd. wt.) and piperazine at a dose of 200mg/kg body weight single dose for seven consecutive days showed gradual increase of efficacy up to day ...
Journal of the Indonesian Tropical Animal Agriculture, 2019
The study aimed at investigating the population, the protein profile and the ultrastructure of adult worms in the intestine of domestic chicken treated using Areca catechu crude aqueous extract. Fifty domestic female chickens of 6 weeks of age were assigned to 5 groups. Group A (negative control) was not given any treatment and any drug. Groups B, C and D were given the treatment at the doses of 26 mg/mL, 53 mg/mL and 79 mg/mL, respectively. Group E (positive control) was given Pyrantel®. Necropsy was conducted to all of the chickens 14 days after the treatment. Adult worms were collected and counted. The worms used in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Scanning Electron Microscopy (SEM) were those collected from the jejunum of the chickens in the groups A, B and C. The biggest number of the worms was found in the jejunum. The results of electrophoresis showed that the dose 53 mg/mL gave fewer protein bands than the negative control (21:12 ratio...
Journal of Parasite Science
The aim of this research was to know whether pineapple peel juice can be used as an ovicidal of in vitro A galli. This study used eggs of A. galli worms obtained by collecting adult A. galli worms from the small intestines of fresh local chickens purchased from traditional markets in Surabaya. The research design used was a completely randomized design (CRD) with four treatments , the concentration of pineapple peel juice was 0% (Control), 5%, 10%, and 20%. Each treatment consisted of six replications, with observations on days 5, 10, 15 and 20. Developed eggs were identified with the presence of larvae within egg and the movement of larvae. The data obtained will be analyzed with the ANOVA Statistical Test with the SPSS device and then followed by the Duncan test. The results of statistical analysis showed that pineapple peel juice had a significant effect on the development of A. galli worm eggs In Vitro. The number of damaged A.galli eggs increased as they were given higher pinea...