Structure-Function Analysis of Human alpha1[IMAGE]3Fucosyltransferases (original) (raw)

Human al-3fucosyltransferases (FucTs) are membrane-bound enzymes critical for fucosylated glycoconjugate synthesis, which play roles in blood group determination, development, and tumorigenesis. This study investigates the inactivation mechanisms of FucTs by N-ethylmaleimide (NEM) and elucidates the importance of specific cysteine residues in enzyme activity and substrate binding. Key findings indicate that certain FucTs are sensitive to inactivation by NEM, with implications for understanding fucosylation regulation in biological processes.