Effect of Benzophenone-3 to Acetylcholinesterase and Antioxidant System in Zebrafish (Danio rerio) Embryos (original) (raw)

Abstract

Benzophenone-3 (BP-3) is one of the most used UV lters. This study aimed to evaluate the toxic effects of BP-3 during embryo stages of zebra sh, four hours post-fertilization (4hpf). Embryos were exposed to 0, 1, and 10 µg L-1 of BP-3, for 72 hours. We investigated biochemical and molecular biomarkers of neurotoxicity (AChE) and the antioxidant system (gene expression of catalase, CAT, superoxide dismutase, SOD, glutathione peroxidase, GPX, the concentration of total glutathione, GSH, and lipid hydroperoxides, LPO). Results indicated that the acute exposure to BP-3 in zebra sh embryos did not show signi cant differences in survival, hatching rate, or in the biomarkers of antioxidant system. In contrast, there were signi cant differences associated with AChE gene expression and activity.

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