Synthesis of M-Ag3PO4, (M = Se, Ag, Ta) Nanoparticles and Their Antibacterial and Cytotoxicity Study (original) (raw)
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In the present study, the silver (Ag) nanoparticles (NPs) were fabricated using pakhoi (p), a traditional alcoholic beverage popularly used in the Garhwal region of Uttarakhand that has been known to possess significant antimicrobial activity properties. Different physicochemical techniques were used to characterize p-Ag NPs. The results confirm the synthesis of crystalline p-Ag NPs having a nearly spherical shape with a net positive charge. Further, p-Ag NPs exhibit strong antibacterial activity against Gram -ve bacteria. Moreover, a detailed study will be beneficial to understanding and exploiting the biomedical application and environmental remediation activity of the p-Ag NPs.
Microscopy Research and Technique, 2020
In the present study, green silver nanoparticles (Ag 2 ONPs) were prepared from aqueous and ethanolic leaves extract of Rhamnus virgata in a facile, green, cost-effective, and eco-friendly way. The color changes from light brown to brownish black determined the synthesis of Ag 2 ONPs (Aq) and Ag 2 ONPs (Et). The phytofabrication of Ag 2 ONPs was confirmed using various spectroscopic and microscopic techniques: energy-dispersive X-ray spectroscopy, dynamic light scattering, ultraviolet-visible spectroscopy, Fouriertransform infrared, X-ray powder diffraction, Raman, scanning electron microscopy, and transmission electron microscopy. Detailed in vitro biological activities determined significant biopotentials for Ag 2 ONPs. The Ag 2 ONPs (Aq) and Ag 2 ONPs (Et) were investigated for anticancer potential against HUH-7 (IC 50 : 9.075 μg/ml for Ag 2 O (Aq) and 25.66 μg/ml for Ag 2 O (Et)) and HepG2 (IC 50 : 25.18 μg/ml for Ag 2 O (Aq) and IC 50 : 27.74 μg/ml for Ag 2 O (Aq)) cell lines. Concentration-dependent cytotoxicity was performed against brineshrimps (IC 50 : 36.04 μg/ml for Ag 2 O (Aq) and 28.82 μg/ml for Ag 2 O (Et)) and Leishmanial parasite (amastigotes and promastigotes). Disc-diffusion method revealed significant antimicrobial activities. In addition, significant enzyme inhibitory activity and antiradical potentials were studied. The hemocompatible nature of Ag 2 ONPs (Aq) and Ag 2 ONPs (Et) was revealed using biocompatibility tests. In conclusion, the green Ag 2 ONPs (Aq) and Ag 2 ONPs (Et) are nontoxic and biocompatible and has shown significant biological activities. We further encourage in vivo studies to ensure biosafety and biocompatibility, so that they can be effectively utilized in nano-pharmaceutical industries.
Hydroxyapatite supported antibacterial Ag3PO4 nanoparticles
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High surface area hydroxyapatites have been explored as biocompatible supports for antibacterial applications. Porosimetry, XRD, XPS and XAS reveal that Ag-doped mesoporous hydroxyapatite promotes the genesis of potent Ag 3 PO 4 nanoparticles, effective against Staphylococcus aureus and Pseudomonas aeruginosa.
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Colloids and Surfaces B: Biointerfaces, 2013
Spherical silver-doped calcium phosphate nanoparticles were synthesized in a co-precipitation route from calcium nitrate/silver nitrate and ammonium phosphate in a continuous process and colloidally stabilized by carboxymethyl cellulose. Nanoparticles with 0.39 wt% silver content and a diameter of about 50-60 nm were obtained. The toxic effects toward mammalian and prokaryotic cells were determined by viability tests and determination of the minimal inhibitory and minimal bactericidal concentrations (MIC and MBC). Three mammalian cells lines, i.e. human mesenchymal stem cells (hMSC) and blood peripheral mononuclear cells (PBMC, monocytes and T-lymphocytes), and two prokaryotic strains, i.e. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used. Silver-doped calcium phosphate nanoparticles and silver acetate showed similar effect toward mammalian and prokaryotic cells with toxic silver concentrations in the range of 1-3 g mL −1 .
Optik, 2018
Present study reports the synthesis of Ag3PO4 nano/mircoparticles by facile precipitation and colloidal synthesis approach in aqueous solution. Importantly, the presented approach does not require complicated instruments, difficult processes, extra activities, and harsh conditions such as high temperature, and pressure. The obtained samples were characterized using SEM with EDS, XRD, TEM with HR-TEM and SAED and FTIR. Their optical properties were studied using Uv-vis diffusive reflectance spectra. Size dependent antibacterial activity towards Gram-positive (S. aureus) and Gram-negative (E. coli, K. pneumoniae and P. aeruginosa) bacteria. Experimental results showed that the antibacterial performances of nanoparticles obtained by mixing 0.01M of each reactant (colloidal synthesis approach) were found to be much higher than that of other formulation under identical conditions.
Evaluation of Biological Activities of Chemically Synthesized Silver Nanoparticles
Journal of Nanomaterials, 2015
Silver nanoparticles were synthesized by the earlier reported methods. The synthesized nanoparticles were characterized using ultraviolet-visible spectrophotometry (UV/Vis), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX), and X-ray powder diffraction (XRD). The synthesized materials were also evaluated for their antibacterial activity against Gram positive and Gram negative bacterial strains. TEM micrograph showed the spherical morphology of AgNPs with size range of 40–60 nm.The synthesized nanoparticles showed a strong antimicrobial activity and their effect depends upon bacterial strain as AgNPs exhibited greater inhibition zone forPseudomonas aeruginosa(19.1 mm) followed byStaphylococcus aureus(14.8 mm) andS. pyogenes(13.6 mm) while the least activity was observed forSalmonella typhi(12.5 mm) at concentration of 5 µg/disc. The minimum inhibitory concentration (MIC) of AgNPs againstS. aureuswas 2.5 µg/disc and less than 2.5 µg/disc forP. aerugin...
Colloids and Interfaces
This study focuses on the synthesis of silver nanoparticles (AgNPs) at different high concentrations and investigates their physicochemical properties, antimicrobial activity, and cytotoxicity. AgNPs were synthesized using the alcohol reduction process, involving the reduction of AgNO3 and its subsequent stabilization via PVP at 80 °C for 4 h. The AgNO3/PVP molar ratio and the average molecular weight were modified in this study. Characterization analyses revealed that the synthesized AgNPs exhibited characteristic surface plasmon resonance absorption peaks at approximately 415 nm, as observed in the UV–Vis spectrum. The results presented in X-ray diffractograms confirmed the face-centered cubic structure of metallic Ag in the nanoparticles. The nanoparticles demonstrated uniform size and shape, with controllable dimensions ranging from 3 to 800 nm. Regarding antimicrobial activity, the MIC solutions exhibited higher potency against the planktonic cells of Candida albicans. The dete...